rpoB RNA polymerase subunit beta [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: rpoB
Gene description: RNA polymerase subunit beta
Primary source: ASAP:ABE-0013041
Locus tag: b3987
See related: ECOCYC:EG10894
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3978; ftsR; groN; nitB; rif; ron; sdgB; stl; stv; tabD
Summary: HT_Cmplx11_Mem: [More information is available at EcoGene: EG10894]. Along with its ' partner, the subunit of is integrally involved in the enzymatic function of RNA polymerase. [More information is available at EcoCyc: EG10894].
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Genomic context

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Sequence:: NC_000913.3 (4181245..4185273)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The C-terminal segment of coliphage HK022 Nun interacts with the RNA polymerase beta and beta' subunits inside the RNA polymerase active site cleft as well as with nearly every element of the nucleic acid scaffold, essentially crosslinking the RNA polymerase and the nucleic acids to prevent translocation.
Title: Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex.
Overexpression of recombinant inhibitor of host bacterial RNA polymerase (Gp2) in exponentially growing E. coli induces changes in RNAP behavior at single molecule level and induces global alterations in the transcriptional programme.
Title: Phenotypic consequences of RNA polymerase dysregulation in Escherichia coli.
The properties of generated RNA polymerase (RNAP) beta subunit variants Iindicate an RNA/protein interaction network that is crucial for transcription, thereby explaining the details of initiation-to-elongation transition on atomic level.
Title: Insights into RNA polymerase catalysis and adaptive evolution gained from mutational analysis of a locus conferring rifampicin resistance.
ppGpp binds to two distinct sites 60 A apart on E. coli RNA polymerase (RNAP), one characterized previously (site 1) and a second identified here at an interface of RNAP and the transcription factor DksA (site 2). Site 2 binding results in the majority of ppGpp's effects on transcription initiation in vitro and in vivo, and strains lacking site 2 are severely impaired for growth following nutritional shifts.
Title: ppGpp Binding to a Site at the RNAP-DksA Interface Accounts for Its Dramatic Effects on Transcription Initiation during the Stringent Response.
The D654Y single amino acid substitution in RpoB rendered E. coli cells resistant to osmotic stress, probably due to improved cell growth and viability via enhanced sugar uptake under stressed conditions, and activated a potential "pre-defense" mechanism under non-stressed conditions. The findings of this work will be useful for bacterial host improvement to enhance its resistance to osmotic stress and facilitate bio-base
Title: A novel point mutation in RpoB improves osmotolerance and succinic acid production in Escherichia coli.
These mutant rpoB RNA polymerases affect rcsA transcription, but per se are not defective either at rcsA or at cps promoters ande hence lead to increased capsular polysaccharide synthesis in Deltalon strains of Escherichia coli.
Title: Suppression of capsule expression in Δlon strains of Escherichia coli by two novel rpoB mutations in concert with HNS: possible role for DNA bending at rcsA promoter.
Genotype-by-environment interactions of rpoB generated by antibiotic resistance mutations are more pronounced after adaptation to other types of environmental variation, making it difficult to predict long-term selection
Title: Genotype-by-environment interactions due to antibiotic resistance and adaptation in Escherichia coli.
analysis of RNA polymerase rpoB mutations that alter transcription slippage during elongation in Escherichia coli
Title: Isolation and characterization of RNA polymerase rpoB mutations that alter transcription slippage during elongation in Escherichia coli.
dG1551 and dC1692 in rpoB are critical for rifampicin drug resistance.
Title: [Mutagenesis of ion beam implantation and identification of two new rifampicin resistance determining sites in rpoB gene in Escherichia coli].
RNA polymerase is able to bind and react with many different RNAs and we suggest that RNAs are involved in transcriptional regulation more frequently than anticipated.
Title: Isolation of small RNA-binding proteins from E. coli: evidence for frequent interaction of RNAs with RNA polymerase.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables 5'-3' RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to DNA-directed 5'-3' RNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-directed 5'-3' RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables nucleotidyltransferase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ribonucleoside binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated transcription initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA-templated transcription initiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in bacterial-type flagellum assembly	NAS Non-traceable Author Statement more info	PubMed
involved_in bacterial-type flagellum-dependent cell motility	NAS Non-traceable Author Statement more info	PubMed
involved_in cell motility	NAS Non-traceable Author Statement more info	PubMed
involved_in cellular response to cell envelope stress	NAS Non-traceable Author Statement more info	PubMed
involved_in intracellular iron ion homeostasis	NAS Non-traceable Author Statement more info	PubMed
involved_in nitrate assimilation	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of DNA-templated transcription elongation	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of DNA-templated transcription initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription initiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to antibiotic	IEA Inferred from Electronic Annotation more info
involved_in response to heat	NAS Non-traceable Author Statement more info	PubMed
involved_in submerged biofilm formation	NAS Non-traceable Author Statement more info	PubMed
involved_in transcription antitermination	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of DNA-directed RNA polymerase complex	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	HDA more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
part_of cytosolic DNA-directed RNA polymerase complex	IPI Inferred from Physical Interaction more info	PubMed
part_of cytosolic DNA-directed RNA polymerase complex	ISS Inferred from Sequence or Structural Similarity more info
located_in membrane	HDA more info	PubMed
part_of transcription elongation factor complex	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: RNA polymerase subunit beta

NP_418414.1

EC 2.7.7.6

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

4181245..4185273

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_418414.1 RNA polymerase subunit beta [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_418414.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A0B5HLZ9, A0A0B5HQP6, A0A6C9QA67, E1CBR5

Conserved Domains (1) summary

PRK00405
Location:1 → 1342

rpoB; DNA-directed RNA polymerase subunit beta; Reviewed