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    diaA DnaA initiator-associating protein DiaA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947661, updated on 4-Feb-2024

    Summary

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    Gene symbol
    diaA
    Gene description
    DnaA initiator-associating protein DiaA
    Primary source
    ASAP:ABE-0010348
    Locus tag
    b3149
    See related
    ECOCYC:EG12780
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3137; yraO
    Summary
    Proper amounts of DiaA are required for the timely initiation of chromosomal replication. [More information is available at EcoCyc: EG12780].
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    Genomic context

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    Sequence:
    NC_000913.3 (3295809..3296399)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 16S rRNA 2'-O-ribose C1402 methyltransferase Neighboring gene outer membrane lipoprotein - activator of MrcA activity Neighboring gene PF02021 family protein YraN Neighboring gene division and outer membrane stress-associated lipid-binding lipoprotein Neighboring gene permease family protein YraQ

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP DnaA-specific initiation complexes. Keyamura K, et al. Genes Dev, 2007 Aug 15. PMID 17699754, Free PMC Article
    2. DiaA, a novel DnaA-binding protein, ensures the timely initiation of Escherichia coli chromosome replication. Ishida T, et al. J Biol Chem, 2004 Oct 29. PMID 15326179
    3. DiaA dynamics are coupled with changes in initial origin complexes leading to helicase loading. Keyamura K, et al. J Biol Chem, 2009 Sep 11. PMID 19632993, Free PMC Article
    4. Regulation of the initiation of chromosomal replication in bacteria. Zakrzewska-Czerwińska J, et al. FEMS Microbiol Rev, 2007 Jul. PMID 17459114
    5. A role for nonessential domain II of initiator protein, DnaA, in replication control. Molt KL, et al. Genetics, 2009 Sep. PMID 19546317, Free PMC Article

    See all (12) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The structural homology between DiaA and HobA strongly supports their involvement in the replication process and these proteins could define a new structural family of replication regulators in bacteria.
      Title: Structural similarity between the DnaA-binding proteins HobA (HP1230) from Helicobacter pylori and DiaA from Escherichia coli.
    2. DiaA regulates initiation by a novel mechanism, in which DiaA tetramers most likely bind to multiple DnaA molecules and stimulate the assembly of specific ATP-DnaA-oriC complexes.
      Title: The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP DnaA-specific initiation complexes.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables carbohydrate derivative binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in carbohydrate derivative metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within positive regulation of DNA-templated DNA replication initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated DNA replication initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of DNA-templated DNA replication initiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DnaA-DiaA complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DnaA initiator-associating protein DiaA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3295809..3296399
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417618.1 DnaA initiator-associating protein DiaA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417618.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AS89
      Conserved Domains (1) summary
      PRK10886
      Location:1 → 196
      PRK10886; DnaA initiator-associating protein DiaA; Provisional
    Nucleotide Protein
    Heading Accession and Version

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