Ube2n ubiquitin-conjugating enzyme E2N [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ube2nprovided by MGI
Official Full Name: ubiquitin-conjugating enzyme E2Nprovided by MGI
Primary source: MGI:MGI:1934835
See related: Ensembl:ENSMUSG00000074781 AllianceGenome:MGI:1934835
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: UBC13; 1500026J17Rik
Summary: Enables ubiquitin conjugating enzyme activity. Involved in protein K63-linked ubiquitination. Acts upstream of or within ubiquitin-dependent protein catabolic process. Located in nucleus. Is expressed in brain. Orthologous to human UBE2N (ubiquitin conjugating enzyme E2 N). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in testis adult (RPKM 37.1), CNS E18 (RPKM 34.6) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 C2; 10 49.41 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (95351024..95381520)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (95515162..95545658)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Deubiquitinase OTUD6A Regulates Innate Immune Response via Targeting UBC13.
Title: Deubiquitinase OTUD6A Regulates Innate Immune Response via Targeting UBC13.
S-Nitrosylation of OTUB1 Alters Its Stability and Ubc13 Binding.
Title: S-Nitrosylation of OTUB1 Alters Its Stability and Ubc13 Binding.
The deubiquitinase OTUB1 augments NF-kappaB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13.
Title: The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13.
Echinococcus multilocularis infection induces UBE2N suppression via exosomal emu-miR-4989.
Title: Echinococcus multilocularis infection induces UBE2N suppression via exosomal emu-miR-4989.
Ubc13 Promotes K63-Linked Polyubiquitination of NLRP3 to Activate Inflammasome.
Title: Ubc13 Promotes K63-Linked Polyubiquitination of NLRP3 to Activate Inflammasome.
Conditional knockout of UBC13 produces disturbances in gait and spontaneous locomotion and exploration in mice.
Title: Conditional knockout of UBC13 produces disturbances in gait and spontaneous locomotion and exploration in mice.
Tetrahydrobiopterin modulates ubiquitin conjugation to UBC13/UBE2N and proteasome activity by S-nitrosation
Title: Tetrahydrobiopterin modulates ubiquitin conjugation to UBC13/UBE2N and proteasome activity by S-nitrosation.
UBE2N is a novel regulator of the MEK/FRA1/SOX10 signaling cascade and is indispensable for malignant melanoma growth
Title: UBE2N Promotes Melanoma Growth via MEK/FRA1/SOX10 Signaling.
Together with Riplet, Ube2D3 promotes covalent conjugation of polyubiquitin chains to RIG-I, while Ube2N preferentially facilitates production of unanchored chains. In the presence of these chains, RIG-I induces MAVS aggregation directly on the mitochondria. Data thus reveal two essential mechanisms underlying the activation of RIG-I and MAVS for triggering innate immune signaling in response to viral infection in cells.
Title: Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity.
Study have shown that Ubc13 haploinsufficiency protects against age-related insulin resistance and high-fat diet-induced obesity.
Title: Ubc13 haploinsufficiency protects against age-related insulin resistance and high-fat diet-induced obesity.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (1) 1 citation
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH125323 (e-PCR)
- UniSTS:162145

BB166398 (e-PCR)
- UniSTS:208598

BB101821 (e-PCR)
- UniSTS:185848

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin conjugating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to ubiquitin conjugating enzyme activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin conjugating enzyme activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin conjugating enzyme activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activator activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IEA Inferred from Electronic Annotation more info
involved_in DNA double-strand break processing	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within DNA repair	IEA Inferred from Electronic Annotation more info
involved_in T cell receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in T cell receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in antiviral innate immune response	ISO Inferred from Sequence Orthology more info
involved_in double-strand break repair via homologous recombination	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of TORC1 signaling	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA repair	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of double-strand break repair	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein K63-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in postreplication repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in postreplication repair	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein K63-linked ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein K63-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K63-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of UBC13-MMS2 complex	ISO Inferred from Sequence Orthology more info
part_of UBC13-UEV1A complex	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info	PubMed
part_of ubiquitin conjugating enzyme complex	ISO Inferred from Sequence Orthology more info
part_of ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: ubiquitin-conjugating enzyme E2 N

Names: E2 ubiquitin-conjugating enzyme N; bendless-like ubiquitin-conjugating enzyme; ubiquitin carrier protein N; ubiquitin-protein ligase N

NP_542127.1

EC 2.3.2.23

XP_006514407.1

EC 2.3.2.23

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_080560.3 → NP_542127.1 ubiquitin-conjugating enzyme E2 N

See identical proteins and their annotated locations for NP_542127.1

Status: VALIDATED

Source sequence(s)

AC159135

Consensus CDS

CCDS48677.1

UniProtKB/Swiss-Prot

P61089, Q3TSL6, Q6ZWZ0, Q9DAJ6

UniProtKB/TrEMBL

A2RTT4

Related

ENSMUSP00000096932.4, ENSMUST00000099329.5

Conserved Domains (1) summary

cl00154
Location:4 → 151

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

95351024..95381520

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006514344.5 → XP_006514407.1 ubiquitin-conjugating enzyme E2 N isoform X1

Conserved Domains (1) summary

cl00154
Location:29 → 170

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...