Spire1 spire type actin nucleation factor 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Spire1provided by MGI
Official Full Name: spire type actin nucleation factor 1provided by MGI
Primary source: MGI:MGI:1915416
See related: Ensembl:ENSMUSG00000024533 AllianceGenome:MGI:1915416
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Spir-1; 6030430B19Rik
Summary: Predicted to enable actin binding activity. Involved in several processes, including cytoskeleton organization; cytoskeleton-dependent cytokinesis; and positive regulation of mitochondrial fission. Acts upstream of or within Golgi vesicle transport; behavioral fear response; and dendritic spine development. Located in Golgi apparatus; cell cortex; and cytoplasmic vesicle membrane. Is integral component of mitochondrial outer membrane. Colocalizes with cleavage furrow. Is expressed in central nervous system; dorsal root ganglion; genitourinary system; liver; and spleen. Orthologous to human SPIRE1 (spire type actin nucleation factor 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in cerebellum adult (RPKM 16.5), frontal lobe adult (RPKM 16.3) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: 18 E1; 18 39.99 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	18	NC_000084.7 (67621279..67745880, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	18	NC_000084.6 (67488209..67612810, complement)

Chromosome 18 - NC_000084.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lnc-SMaRT Translational Regulation of Spire1, A New Player in Muscle Differentiation.
Title: Lnc-SMaRT Translational Regulation of Spire1, A New Player in Muscle Differentiation.
Two mechanisms drive pronuclear migration in mouse zygotes.
Title: Two mechanisms drive pronuclear migration in mouse zygotes.
After injection of double-strand RNA against Spire 1 and 2 (Spire1+2 double-strand RNA), actin mesh levels were significantly decreased compared with that of control (negative).
Title: Spire localization via zinc finger-containing domain is crucial for the asymmetric division of mouse oocyte.
Spire-1 is specifically recruited at invadosomes and is part of a multi-molecular complex containing Src kinase, the formin mDia1 and actin.
Title: Spire-1 contributes to the invadosome and its associated invasive properties.
analysis of the molecular basis of the Spir1/formin-2 interaction
Title: Molecular basis of actin nucleation factor cooperativity: crystal structure of the Spir-1 kinase non-catalytic C-lobe domain (KIND)•formin-2 formin SPIR interaction motif (FSI) complex.
Spire1 and Spire2 drive two critical steps of asymmetric oocyte division.
Title: Spire-type actin nucleators cooperate with Formin-2 to drive asymmetric oocyte division.
By analyzing the expression of the spir-1 gene it was shown that spir-1 and formin-2 have a nearly identical expression pattern during mouse embryogenesis and in the adult brain.
Title: Overlapping expression pattern of the actin organizers Spir-1 and formin-2 in the developing mouse nervous system and the adult brain.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)

General gene information

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Markers

NoName (e-PCR)
- UniSTS:237829

Spire1 (e-PCR), detects polymorphism
- UniSTS:281114

D19373 (e-PCR)
- UniSTS:124810

AW550622 (e-PCR)
- UniSTS:181139

Spire1 (e-PCR), detects polymorphism
- UniSTS:531430

AU022898 (e-PCR)
- UniSTS:209393

AI415299 (e-PCR)
- UniSTS:160757

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in Golgi vesicle transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within Golgi vesicle transport	IDA Inferred from Direct Assay more info	PubMed
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in actin filament network formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament polymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin nucleation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within behavioral fear response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cleavage furrow formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cleavage furrow formation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within dendritic spine development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of meiotic spindle localization	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of meiotic spindle localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of meiotic spindle localization	ISO Inferred from Sequence Orthology more info
involved_in formin-nucleated actin cable assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in formin-nucleated actin cable assembly	ISO Inferred from Sequence Orthology more info
involved_in intracellular transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in polar body extrusion after meiotic divisions	IBA Inferred from Biological aspect of Ancestor more info
involved_in polar body extrusion after meiotic divisions	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of double-strand break repair	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mitochondrial fission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitochondrial fission	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein transport	IEA Inferred from Electronic Annotation more info
involved_in vesicle-mediated transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
is_active_in cell cortex	IBA Inferred from Biological aspect of Ancestor more info
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with cleavage furrow	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasmic vesicle membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic vesicle membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein spire homolog 1

Names: spire homolog 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001403659.1 → NP_001390588.1 protein spire homolog 1 isoform 3

Status: VALIDATED

Source sequence(s)

AC120410
NM_001403660.1 → NP_001390589.1 protein spire homolog 1 isoform 4

Status: VALIDATED

Source sequence(s)

AC120410
NM_001403661.1 → NP_001390590.1 protein spire homolog 1 isoform 5

Status: VALIDATED

Source sequence(s)

AC120410

Related

ENSMUSP00000080871.7, ENSMUST00000082243.7
NM_176832.2 → NP_789802.1 protein spire homolog 1 isoform 2

See identical proteins and their annotated locations for NP_789802.1

Status: VALIDATED

Source sequence(s)

AC120410

Consensus CDS

CCDS37849.1

UniProtKB/Swiss-Prot

Q52KF3, Q6PDJ5, Q80V45

UniProtKB/TrEMBL

A0A286YDN9

Related

ENSMUSP00000153492.2, ENSMUST00000224799.2

Conserved Domains (1) summary

cd15767
Location:347 → 467

FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins
NM_194355.3 → NP_919336.2 protein spire homolog 1 isoform 1

Status: VALIDATED

Source sequence(s)

AC120410

Conserved Domains (2) summary

cd15767
Location:620 → 740

FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins

pfam16474
Location:24 → 223

KIND; Kinase non-catalytic C-lobe domain

RNA

NR_175397.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC120410

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000084.7 Reference GRCm39 C57BL/6J

Range

67621279..67745880 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006526206.2 → XP_006526269.1 protein spire homolog 1 isoform X4

UniProtKB/Swiss-Prot

Q52KF3, Q6PDJ5, Q80V45

UniProtKB/TrEMBL

A0A5H1ZRL1

Related

ENSMUSP00000110702.4, ENSMUST00000115050.10

Conserved Domains (2) summary

cd15767
Location:575 → 695

FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins

pfam16474
Location:24 → 223

KIND; Kinase non-catalytic C-lobe domain
XM_006526203.5 → XP_006526266.1 protein spire homolog 1 isoform X2

Conserved Domains (2) summary

cd15767
Location:633 → 753

FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins

pfam16474
Location:24 → 223

KIND; Kinase non-catalytic C-lobe domain
XM_006526209.5 → XP_006526272.1 protein spire homolog 1 isoform X1

Conserved Domains (2) summary

cd15767
Location:634 → 754

FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins

pfam16474
Location:24 → 223

KIND; Kinase non-catalytic C-lobe domain
XM_030250590.2 → XP_030106450.1 protein spire homolog 1 isoform X7

UniProtKB/Swiss-Prot

Q52KF3, Q6PDJ5, Q80V45

UniProtKB/TrEMBL

D3YTL8

Related

ENSMUSP00000049336.7, ENSMUST00000045105.13

Conserved Domains (2) summary

cd15767
Location:475 → 595

FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins

cl21453
Location:1 → 78

PKc_like; Protein Kinases, catalytic domain
XM_030250587.2 → XP_030106447.1 protein spire homolog 1 isoform X6

UniProtKB/Swiss-Prot

Q52KF3, Q6PDJ5, Q80V45

Conserved Domains (2) summary

cd15767
Location:489 → 609

FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins

cl21453
Location:1 → 78

PKc_like; Protein Kinases, catalytic domain
XM_030250586.2 → XP_030106446.1 protein spire homolog 1 isoform X6

UniProtKB/Swiss-Prot

Q52KF3, Q6PDJ5, Q80V45

Conserved Domains (2) summary

cd15767
Location:489 → 609

FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins

cl21453
Location:1 → 78

PKc_like; Protein Kinases, catalytic domain
XM_030250589.2 → XP_030106449.1 protein spire homolog 1 isoform X7

UniProtKB/Swiss-Prot

Q52KF3, Q6PDJ5, Q80V45

UniProtKB/TrEMBL

D3YTL8

Conserved Domains (2) summary

cd15767
Location:475 → 595

FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins

cl21453
Location:1 → 78

PKc_like; Protein Kinases, catalytic domain