Prkce protein kinase C, epsilon [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Prkceprovided by RGD
Official Full Name: protein kinase C, epsilonprovided by RGD
Primary source: RGD:61925
See related: Ensembl:ENSRNOG00000015603 AllianceGenome:RGD:61925
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Pkce
Summary: Enables several functions, including SH3 domain binding activity; protein kinase C activity; and protein kinase binding activity. Involved in several processes, including cellular response to platelet-derived growth factor stimulus; negative regulation of mitochondrial calcium ion concentration; and negative regulation of mitochondrial membrane potential. Located in Golgi membrane; T-tubule; and cytosol. Is active in glutamatergic synapse; neuromuscular junction; and presynaptic cytosol. Used to study hypertension. Biomarker of type 2 diabetes mellitus. Orthologous to human PRKCE (protein kinase C epsilon). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 183.2), Heart (RPKM 82.1) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 6q12

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	6	NC_086024.1 (13718050..14204931, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	6	NC_051341.1 (7965048..8451966, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	6	NC_005105.4 (9483400..9973396)

Chromosome 6 - NC_086024.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PKCepsilon controls the fusion of secretory vesicles in mast cells in a phosphatidic acid-dependent mode.
Title: PKCε controls the fusion of secretory vesicles in mast cells in a phosphatidic acid-dependent mode.
Ischemic Neuroprotectant PKCepsilon Restores Mitochondrial Glutamate Oxaloacetate Transaminase in the Neuronal NADH Shuttle after Ischemic Injury.
Title: Ischemic Neuroprotectant PKCε Restores Mitochondrial Glutamate Oxaloacetate Transaminase in the Neuronal NADH Shuttle after Ischemic Injury.
Activated Protein Kinase C (PKC) Is Persistently Trafficked with Epidermal Growth Factor (EGF) Receptor.
Title: Activated Protein Kinase C (PKC) Is Persistently Trafficked with Epidermal Growth Factor (EGF) Receptor.
PKCepsilon associates with the Kv3.4 channel to promote its expression in a kinase activity-dependent manner.
Title: PKCε associates with the Kv3.4 channel to promote its expression in a kinase activity-dependent manner.
Conjugated linoleic acid isomers impair insulin-induced Akt phosphorylation through activation of Pkce.
Title: Isomers of conjugated linoleic acid induce insulin resistance through a mechanism involving activation of protein kinase Cε in liver cells.
nPKCepsilon isoform is essential to positively regulate SNAP-25 phosphorylation on Ser-187.
Title: nPKCε Mediates SNAP-25 Phosphorylation of Ser-187 in Basal Conditions and After Synaptic Activity at the Neuromuscular Junction.
Serine 302 in cardiac myosin binding protein-C was identified as a PKCepsilon substrate that when phosphorylated contributed to the suppression of contraction and Ca(2+) transients by PKCepsilon following ET-1 stimulation.
Title: Contractile responses to endothelin-1 are regulated by PKC phosphorylation of cardiac myosin binding protein-C in rat ventricular myocytes.
profilin binds to activated PKCepsilon and facilitates PKCepsilon activation by accelerating ATP supply to PKCepsilon.
Title: Profilin facilitates PKCε activation by accelerating ATP supply.
The present results suggested that the different cardiac hemodynamic overload could differentially activate a common signaling, PKCepsilon intermediate and thereby generate biological diversity.
Title: Responses of PKCε to cardiac overloads on myocardial sympathetic innervation and NET expression.
Results suggest that PKCvarepsilon is required to generate Ca(2+) oscillations following mGluR5 activation, which support the regulation of astrocytic glutamate uptake. Reduced expression of astrocytic PKCvarepsilon could impair this neuroprotective process and participate in the progression of amyotrophic lateral sclerosis (ALS).
Title: Constitutive downregulation protein kinase C epsilon in hSOD1(G93A) astrocytes influences mGluR5 signaling and the regulation of glutamate uptake.

Submit: New GeneRIF Correction See all GeneRIFs (170)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AL033630 (e-PCR)
- UniSTS:93462

RH132957 (e-PCR)
- UniSTS:216240

RH140883 (e-PCR)
- UniSTS:222899

D6Rat211 (e-PCR), detects polymorphism
- UniSTS:228048

RH133520 (e-PCR)
- UniSTS:216803

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 14-3-3 protein binding	IEA Inferred from Electronic Annotation more info
enables 14-3-3 protein binding	ISO Inferred from Sequence Orthology more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables SH3 domain binding	IPI Inferred from Physical Interaction more info	PubMed
enables actin monomer binding	IEA Inferred from Electronic Annotation more info
enables actin monomer binding	ISO Inferred from Sequence Orthology more info
enables actin monomer binding	ISS Inferred from Sequence or Structural Similarity more info
enables diacylglycerol-dependent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables diacylglycerol-dependent serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables diacylglycerol-dependent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables enzyme activator activity	IEA Inferred from Electronic Annotation more info
enables enzyme activator activity	ISO Inferred from Sequence Orthology more info
enables enzyme binding	IEA Inferred from Electronic Annotation more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables ethanol binding	IEA Inferred from Electronic Annotation more info
enables ethanol binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor activator activity	IEA Inferred from Electronic Annotation more info
enables signaling receptor activator activity	ISO Inferred from Sequence Orthology more info
enables signaling receptor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in MAPK cascade	IEA Inferred from Electronic Annotation more info
acts_upstream_of MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in TRAM-dependent toll-like receptor 4 signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in TRAM-dependent toll-like receptor 4 signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in TRAM-dependent toll-like receptor 4 signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in cell-substrate adhesion	IEA Inferred from Electronic Annotation more info
acts_upstream_of cell-substrate adhesion	ISO Inferred from Sequence Orthology more info
involved_in cellular response to ethanol	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to ethanol	ISO Inferred from Sequence Orthology more info
involved_in cellular response to hypoxia	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to hypoxia	ISO Inferred from Sequence Orthology more info
involved_in cellular response to platelet-derived growth factor stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to prostaglandin E stimulus	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to prostaglandin E stimulus	ISO Inferred from Sequence Orthology more info
involved_in establishment of localization in cell	IEA Inferred from Electronic Annotation more info
acts_upstream_of establishment of localization in cell	ISO Inferred from Sequence Orthology more info
involved_in insulin secretion	IEA Inferred from Electronic Annotation more info
acts_upstream_of insulin secretion	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in lipopolysaccharide-mediated signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipopolysaccharide-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in lipopolysaccharide-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in lipopolysaccharide-mediated signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in locomotory exploration behavior	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within locomotory exploration behavior	ISO Inferred from Sequence Orthology more info
involved_in macrophage activation involved in immune response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within macrophage activation involved in immune response	ISO Inferred from Sequence Orthology more info
involved_in mucus secretion	IEA Inferred from Electronic Annotation more info
acts_upstream_of mucus secretion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of mitochondrial calcium ion concentration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of mitochondrial membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of release of sequestered calcium ion into cytosol	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-serine phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of MAPK cascade	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of actin filament polymerization	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of actin filament polymerization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of actin filament polymerization	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of canonical NF-kappaB signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell-substrate adhesion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of cell-substrate adhesion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cellular glucuronidation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cellular glucuronidation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cytokinesis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cytokinesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cytokinesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of epithelial cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of epithelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of epithelial cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of fibroblast migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of fibroblast migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of fibroblast migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of insulin secretion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of lipid catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of lipid catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mucus secretion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of mucus secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of synaptic transmission, GABAergic	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of synaptic transmission, GABAergic	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of wound healing	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of wound healing	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of wound healing	ISS Inferred from Sequence or Structural Similarity more info
involved_in presynaptic modulation of chemical synaptic transmission	EXP Inferred from Experiment more info	PubMed
involved_in presynaptic modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of insulin secretion involved in cellular response to glucose stimulus	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of insulin secretion involved in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of lipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of peptidyl-tyrosine phosphorylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of peptidyl-tyrosine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of release of sequestered calcium ion into cytosol	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of release of sequestered calcium ion into cytosol	ISO Inferred from Sequence Orthology more info
involved_in response to morphine	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to morphine	ISO Inferred from Sequence Orthology more info
involved_in synaptic transmission, GABAergic	IEA Inferred from Electronic Annotation more info
acts_upstream_of synaptic transmission, GABAergic	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Golgi apparatus	ISS Inferred from Sequence or Structural Similarity more info
located_in Golgi membrane	IDA Inferred from Direct Assay more info	PubMed
located_in T-tubule	IDA Inferred from Direct Assay more info	PubMed
located_in cell periphery	ISO Inferred from Sequence Orthology more info
located_in cell periphery	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in intermediate filament cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in intermediate filament cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
is_active_in neuromuscular junction	EXP Inferred from Experiment more info	PubMed
is_active_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein kinase C epsilon type

Names: nPKC-epsilon

NP_058867.2

EC 2.7.11.13

XP_038967755.1

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_017171.2 → NP_058867.2 protein kinase C epsilon type

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000006

UniProtKB/Swiss-Prot

P09216

UniProtKB/TrEMBL

F1LMV8, Q6DUV1

Related

ENSRNOP00000020959.8, ENSRNOT00000020959.8

Conserved Domains (4) summary

cd04014
Location:3 → 135

C2_PKC_epsilon; C2 domain in Protein Kinase C (PKC) epsilon

cd05591
Location:412 → 732

STKc_nPKC_epsilon; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C epsilon

cd20835
Location:161 → 224

C1_nPKC_epsilon-like_rpt1; first protein kinase C conserved region 1 (C1 domain) found in novel protein kinase C (nPKC) epsilon, eta, and similar proteins

cd20838
Location:241 → 295

C1_nPKC_epsilon-like_rpt2; second protein kinase C conserved region 1 (C1 domain) found in novel protein kinase C (nPKC) epsilon, eta, and similar proteins

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086024.1 Reference GRCr8

Range

13718050..14204931 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039111827.2 → XP_038967755.1 protein kinase C epsilon type isoform X1

UniProtKB/Swiss-Prot

P09216

Conserved Domains (4) summary

cd04014
Location:3 → 135

C2_PKC_epsilon; C2 domain in Protein Kinase C (PKC) epsilon

cd20835
Location:161 → 224

C1_nPKC_epsilon-like_rpt1; first protein kinase C conserved region 1 (C1 domain) found in novel protein kinase C (nPKC) epsilon, eta, and similar proteins

cd20838
Location:241 → 295

C1_nPKC_epsilon-like_rpt2; second protein kinase C conserved region 1 (C1 domain) found in novel protein kinase C (nPKC) epsilon, eta, and similar proteins

cl21453
Location:412 → 689

PKc_like; Protein Kinases, catalytic domain