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    Ntan1 N-terminal Asn amidase [ Mus musculus (house mouse) ]

    Gene ID: 18203, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Ntan1provided by MGI
    Official Full Name
    N-terminal Asn amidaseprovided by MGI
    Primary source
    MGI:MGI:108471
    See related
    Ensembl:ENSMUSG00000022681 AllianceGenome:MGI:108471
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    PNAA; PNAD
    Summary
    The protein encoded by this gene functions in a step-wise protein degradation process through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asparagine residues on proteins to produce N-terminal L-Aspartate. L-Aspartate substrates are subsequently conjugated to L-Arginine, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Mice with a knock-out of this gene are viable, fertile, and outwardly normal, but show impairments in spontaneous activity and spatial memory, relative to their wild-type counterparts. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
    Expression
    Ubiquitous expression in CNS E14 (RPKM 21.0), CNS E18 (RPKM 20.8) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    16 A1; 16 9.64 cM
    Exon count:
    10
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 16 NC_000082.7 (13636629..13653314)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 16 NC_000082.6 (13818834..13835450)

    Chromosome 16 - NC_000082.7Genomic Context describing neighboring genes Neighboring gene elongin-B pseudogene Neighboring gene RRN3 RNA polymerase I transcription factor homolog (yeast) Neighboring gene pyridoxal-dependent decarboxylase domain containing 1 Neighboring gene nudix (nucleoside diphosphate linked moiety X)-type motif 4 pseudogene Neighboring gene STARR-seq mESC enhancer starr_40163 Neighboring gene STARR-positive B cell enhancer ABC_E8619 Neighboring gene Mpv17 transgene, kidney disease mutant-like Neighboring gene laminin, gamma 1 pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. The magnetism responsive gene Ntan1 in mouse brain. Goto Y, et al. Neurochem Int, 2006 Sep. PMID 16600435
    2. Facilitated stimulus-response associative learning and long-term memory in mice lacking the NTAN1 amidase of the N-end rule pathway. Balogh SA, et al. Brain Res, 2001 Feb 23. PMID 11172781
    3. Varying intertrial interval reveals temporally defined memory deficits and enhancements in NTAN1-deficient mice. Balogh SA, et al. Learn Mem, 2000 Sep-Oct. PMID 11040259, Free PMC Article
    4. Behavioral characterization of mice lacking the ubiquitin ligase UBR1 of the N-end rule pathway. Balogh SA, et al. Genes Brain Behav, 2002 Nov. PMID 12882367
    5. A mouse amidase specific for N-terminal asparagine. The gene, the enzyme, and their function in the N-end rule pathway. Grigoryev S, et al. J Biol Chem, 1996 Nov 8. PMID 8910481

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (1)  1 citation
    • Endonuclease-mediated (1) 

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein-N-terminal asparagine amidohydrolase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein-N-terminal asparagine amidohydrolase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein-N-terminal asparagine amidohydrolase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within adult locomotory behavior IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within memory IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ubiquitin-dependent protein catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ubiquitin-dependent protein catabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    protein N-terminal asparagine amidohydrolase
    Names
    asparagine-specific N-terminal amidase
    protein N-terminal Asn amidase
    protein NH2-terminal asparagine amidohydrolase
    protein NH2-terminal asparagine deamidase
    protein NTN-amidase
    NP_001333035.1
    NP_001333036.1
    NP_001333037.1
    NP_001333038.1
    NP_001333039.1
    NP_001333040.1
    NP_001412925.1
    NP_001412926.1
    NP_001412927.1
    NP_001412928.1
    NP_001412929.1
    NP_001412930.1
    NP_035076.1
    XP_030104855.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001346106.1 → NP_001333035.1  protein N-terminal asparagine amidohydrolase isoform 2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
      Source sequence(s)
      AC107769, AK041112, AW492524, BY168976, BY254590
      Consensus CDS
      CCDS88878.1
      UniProtKB/TrEMBL
      D3YZ99, Q8BRX4
      Related
      ENSMUSP00000111472.3, ENSMUST00000115805.3

    2. NM_001346107.1 → NP_001333036.1  protein N-terminal asparagine amidohydrolase isoform 3

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) uses alternate splice sites in the 3' coding region which result in a frameshift, compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1.
      Source sequence(s)
      AC107769, AK041112, AW492524, BY168976, BY254590
      UniProtKB/TrEMBL
      Q8BRX4

    3. NM_001346108.1 → NP_001333037.1  protein N-terminal asparagine amidohydrolase isoform 4

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) differs in the 5' UTR, uses an alternate start codon, and lacks an alternate exon in the 5' coding region, compared to variant 1. The encoded isoform (4) is shorter and has a distinct N-terminus, compared to isoform 1.
      Source sequence(s)
      AC107769, AW492524, BG070912, BY254590, CK617520
      Related
      ENSMUST00000136618.8

    4. NM_001346109.1 → NP_001333038.1  protein N-terminal asparagine amidohydrolase isoform 5

      Status: REVIEWED

      Description
      Transcript Variant: This variant (5) differs in the 5' UTR, uses an alternate start codon, and lacks two alternate exons in the 5' coding region, compared to variant 1. The encoded isoform (5) is shorter and has a distinct N-terminus, compared to isoform 1.
      Source sequence(s)
      AC107769, AK041112, AW492524, BY254590, CJ057344, CK617520
      UniProtKB/TrEMBL
      Q8BRX4

    5. NM_001346110.1 → NP_001333039.1  protein N-terminal asparagine amidohydrolase isoform 6

      Status: REVIEWED

      Description
      Transcript Variant: This variant (6) differs in the 5' UTR, uses an alternate start codon, and lacks two alternate exons in the coding region, compared to variant 1. The encoded isoform (6) is shorter and has a distinct N-terminus, compared to isoform 1.
      Source sequence(s)
      AC107769, AW492524, BY254590, CK617520

    6. NM_001346111.1 → NP_001333040.1  protein N-terminal asparagine amidohydrolase isoform 7

      Status: REVIEWED

      Description
      Transcript Variant: This variant (7) differs in the 5' UTR, uses an alternate start codon, and lacks three alternate exons in the coding region, compared to variant 1. The encoded isoform (7) is shorter and has a distinct N-terminus, compared to isoform 1.
      Source sequence(s)
      AC107769, AW492524, BY254590, CJ057344, CK617520

    7. NM_001425996.1 → NP_001412925.1  protein N-terminal asparagine amidohydrolase isoform 8

      Status: REVIEWED

      Source sequence(s)
      AC107769

    8. NM_001425997.1 → NP_001412926.1  protein N-terminal asparagine amidohydrolase isoform 9

      Status: REVIEWED

      Source sequence(s)
      AC107769

    9. NM_001425998.1 → NP_001412927.1  protein N-terminal asparagine amidohydrolase isoform 10

      Status: REVIEWED

      Source sequence(s)
      AC107769

    10. NM_001425999.1 → NP_001412928.1  protein N-terminal asparagine amidohydrolase isoform 11

      Status: REVIEWED

      Source sequence(s)
      AC107769

    11. NM_001426000.1 → NP_001412929.1  protein N-terminal asparagine amidohydrolase isoform 12

      Status: REVIEWED

      Source sequence(s)
      AC107769

    12. NM_001426001.1 → NP_001412930.1  protein N-terminal asparagine amidohydrolase isoform 13

      Status: REVIEWED

      Source sequence(s)
      AC107769

    13. NM_010946.4 → NP_035076.1  protein N-terminal asparagine amidohydrolase isoform 1

      See identical proteins and their annotated locations for NP_035076.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (1).
      Source sequence(s)
      AC107769, AK041112, AW492524, BY168976, BY254590
      Consensus CDS
      CCDS27969.1
      UniProtKB/Swiss-Prot
      Q64311
      UniProtKB/TrEMBL
      Q8BRX4
      Related
      ENSMUSP00000023362.9, ENSMUST00000023362.15
      Conserved Domains (1) summary
      pfam14736
      Location:39 → 304
      N_Asn_amidohyd; Protein N-terminal asparagine amidohydrolase

    RNA

    1. NR_144374.1 RNA Sequence

      Status: REVIEWED

      Description
      Transcript Variant: This variant (8) lacks two alternate internal exons and uses an alternate splice site, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      AC107769, AW492524, BY254590, CK617520

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000082.7 Reference GRCm39 C57BL/6J

      Range
      13636629..13653314
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_030248995.2 → XP_030104855.1  protein N-terminal asparagine amidohydrolase isoform X2

      Conserved Domains (1) summary
      pfam14736
      Location:54 → 278
      N_Asn_amidohyd; Protein N-terminal asparagine amidohydrolase
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q64311.3 GenPept UniProtKB/Swiss-Prot:Q64311

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