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    PolA1 DNA polymerase alpha subunit 1 [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 42553, updated on 10-May-2024

    Summary

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    Official Symbol
    PolA1provided by FlyBase
    Official Full Name
    DNA polymerase alpha subunit 1provided by FlyBase
    Primary source
    FLYBASE:FBgn0259113
    Locus tag
    Dmel_CG6349
    See related
    AllianceGenome:FB:FBgn0259113
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    183 kDa pol alpha; AAB24152; CG6349; Ctf4-polalpha; Dmel\CG6349; Dmpol alpha; DmPolalpha; DNA pol alpha; DNA Pol-alpha180; DNA polalpha; DNA Polalpha-180; DNA polymerase alpha 180 kD; DNA-Polalpha; DNApol; DNApol-alpha; DNApol-alpha180; DNApola; DNApolalpha; DNApolalpha180; dpolalpha; dpolalphap180; E(mus304); Pol; pol alpha; Pol alpha; Pol-alpha180; POLA; polalpha; Polalpha; Polalpha 180KDa; POlalpha 180kDa; Polalpha180; polalphap180
    Summary
    Enables 3'-5'-exodeoxyribonuclease activity and DNA-directed DNA polymerase activity. Involved in DNA replication proofreading. Located in nucleus. Part of alpha DNA polymerase:primase complex. Is expressed in follicle cell; nurse cell; organism; and ovary. Human ortholog(s) of this gene implicated in Van Esch-O'Driscoll syndrome and X-linked reticulate pigmentary disorder. Orthologous to human POLA1 (DNA polymerase alpha 1, catalytic subunit). [provided by Alliance of Genome Resources, Apr 2022]
    Orthologs
    all
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    Genomic context

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    See PolA1 in Genome Data Viewer
    Location:
    93F2-93F2; 3-73 cM
    Exon count:
    6
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3R NT_033777.3 (21666562..21671750, complement)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3R NT_033777.2 (17492284..17497472, complement)

    Chromosome 3R - NT_033777.3Genomic Context describing neighboring genes Neighboring gene E2F transcription factor 1 Neighboring gene long non-coding RNA:CR46057 Neighboring gene archease Neighboring gene Juvenile hormone binding protein 16 Neighboring gene uncharacterized protein Neighboring gene long non-coding RNA:CR44096

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Identification of the specific labile sites in the 180 kDa catalytic polypeptide of the Drosophila melanogaster DNA polymerase alpha-primase complex. Kuroda K, et al. J Biochem, 1993 Feb. PMID 8468316
    2. A DNA polymerase alpha-associated 56 kDa protein kinase. Peck VM, et al. Biochem Biophys Res Commun, 1993 Jan 29. PMID 8427578
    3. Mapping an initiation region of DNA replication at a single-copy chromosomal locus in Drosophila melanogaster cells by two-dimensional gel methods and PCR-mediated nascent-strand analysis: multiple replication origins in a broad zone. Shinomiya T, et al. Mol Cell Biol, 1994 Nov. PMID 7935453, Free PMC Article
    4. Immunological comparison of purified DNA polymerase alpha from embryos of Drosophila melanogaster with forms of the enzyme present in vivo. Sauer B, et al. J Biol Chem, 1982 Oct 25. PMID 6811594
    5. Purification and properties of an accessory protein for DNA polymerase alpha/primase. Goulian M, et al. J Biol Chem, 1990 Aug 5. PMID 2165497

    See all (120) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables 3'-5'-DNA exonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA exonuclease activity, producing 5'-phosphomonoesters IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA replication origin binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-directed DNA polymerase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-directed DNA polymerase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-directed DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables chromatin binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables single-stranded DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication proofreading IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA-templated DNA replication IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in lagging strand elongation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in leading strand elongation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mitotic DNA replication initiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mitotic DNA replication initiation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of alpha DNA polymerase:primase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of alpha DNA polymerase:primase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA polymerase alpha subunit 1
    Names
    180 kDa subunit
    180 kDa subunit of DNA polymerase alpha
    180 kDa subunit of polymerase-primase
    182-kDa polymerase subunit
    CG6349-PA
    DNA pol 180
    DNA polymerase 180K
    DNA polymerase alpha
    DNA polymerase alpha 148,000 alpha subunit
    DNA polymerase alpha 180 kD
    DNA polymerase alpha 180-kDa subunit
    DNA polymerase alpha 180kD
    DNA polymerase alpha 180kD subunit
    DNA polymerase alpha 182,000 alpha subunit
    DNA polymerase-alpha p180 subunit
    DNA polymerase-primase 180 kda subunit
    DNA-polymerase alpha
    DNApol-alpha180
    PolA1-PA
    enhancer of mus304
    NP_536736.2

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033777.3 Reference assembly

      Range
      21666562..21671750 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_080488.3 → NP_536736.2  DNA polymerase alpha subunit 1 [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_536736.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O77034, P26019, Q8T992, Q9VD90
      Related
      FBpp0083514
      Conserved Domains (5) summary
      cd05776
      Location:567 → 789
      DNA_polB_alpha_exo; inactive DEDDy 3'-5' exonuclease domain of eukaryotic DNA polymerase alpha, a family-B DNA polymerase
      cd05532
      Location:852 → 1254
      POLBc_alpha; DNA polymerase type-B alpha subfamily catalytic domain. Three DNA-dependent DNA polymerases type B (alpha, delta, and epsilon) have been identified as essential for nuclear DNA replication in eukaryotes. DNA polymerase (Pol) alpha is almost exclusively ...
      TIGR00592
      Location:52 → 1237
      pol2; DNA polymerase (pol2)
      pfam08996
      Location:1286 → 1459
      zf-DNA_Pol; DNA Polymerase alpha zinc finger
      pfam12254
      Location:24 → 83
      DNA_pol_alpha_N; DNA polymerase alpha subunit p180 N terminal
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P26019.2 P26019

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