Mylk2 myosin light chain kinase 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Mylk2provided by RGD
Official Full Name: myosin light chain kinase 2provided by RGD
Primary source: RGD:620934
See related: Ensembl:ENSRNOG00000008235 AllianceGenome:RGD:620934
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Klmc; Mlck
Summary: Enables myosin light chain binding activity and myosin light chain kinase activity. Involved in several processes, including positive regulation of fast-twitch skeletal muscle fiber contraction; regulation of neuronal synaptic plasticity; and synaptic vesicle transport. Located in several cellular components, including dendritic spine; postsynaptic density; and synaptic vesicle. Human ortholog(s) of this gene implicated in hypertrophic cardiomyopathy 1. Orthologous to human MYLK2 (myosin light chain kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward (RPKM 1336.8) See more
Orthologs: human mouse all
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Genomic context

Location:: 3q41

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (161836705..161848609)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (141376450..141388357)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (148386185..148397851)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Down-regulation of miR-200c attenuates AngII-induced cardiac hypertrophy via targeting the MLCK-mediated pathway.
Title: Down-regulation of miR-200c attenuates AngII-induced cardiac hypertrophy via targeting the MLCK-mediated pathway.
MLCK regulates Schwann cell cytoskeletal organization, cell differentiation and myelination.
Title: MLCK regulates Schwann cell cytoskeletal organization, differentiation and myelination.
Src and MLCK form a swelling-induced protein complex that regulates volume recovery through membrane turnover and compensatory endocytosis under osmotic stress.
Title: Myosin light chain kinase and Src control membrane dynamics in volume recovery from cell swelling.
PKC and MLCK pathways important in coronary endothelial dysfunction elicited by ischemia/reperfusion injury and cytokine release
Title: PKC and MLCK-dependent, cytokine-induced rat coronary endothelial dysfunction.
The regulation of smMLCK expression by angiotensin II via Ras signaling is important in the regulation of vascular remodeling and blood pressure
Title: Regulation of myosin light chain kinase expression by angiotensin II in hypertension.
AG repeats in the spontaneously hypertensive rats promoter increase H-DNA structures, histone modifications, and promoter activity of the smooth muscle myosin light chain kinase, perhaps contributing to vascular disorders in hypertension
Title: Naturally extended CT . AG repeats increase H-DNA structures and promoter activity in the smooth muscle myosin light chain kinase gene.
ML-7 and apocynin blocked hypoxia-dependent phosphorylation of MLC. Our data demonstrate that hypoxia causes a breakdown of the BBB in vitro and in vivo involving ROS and the contractile machinery.
Title: Inhibition of the myosin light chain kinase prevents hypoxia-induced blood-brain barrier disruption.
Rho-kinase and MLC kinase play a role in migratory defect in FAK null cells.
Title: Roles of Rho-associated kinase and myosin light chain kinase in morphological and migratory defects of focal adhesion kinase-null cells.
These data provide novel insight into the regulation of smooth muscle myosin light chain kinase (smMLCK) expression at the molecular level and demonstrate the importance of serum response factor (SRF) in regulating smMLCK promoter activity.
Title: Increased myosin light chain kinase expression in hypertension: Regulation by serum response factor via an insertion mutation in the promoter.
in rat skeletal muscles an increase of MLC2 phosphorylation is associated with the slow-to-fast transition regardless of whether hypertrophy or atrophy develops.
Title: Increased phosphorylation of myosin light chain associated with slow-to-fast transition in rat soleus.

Submit: New GeneRIF Correction See all GeneRIFs (13)

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables myosin light chain binding	IBA Inferred from Biological aspect of Ancestor more info
enables myosin light chain binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables myosin light chain kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables myosin light chain kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables myosin light chain kinase activity	IEA Inferred from Electronic Annotation more info
enables myosin light chain kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables myosin light chain kinase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cardiac muscle tissue morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in cardiac muscle tissue morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in cardiac muscle tissue morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in muscle contraction	IEP Inferred from Expression Pattern more info	PubMed
involved_in neuromuscular synaptic transmission	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within neuromuscular synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of fast-twitch skeletal muscle fiber contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in regulation of MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of muscle filament sliding	IEA Inferred from Electronic Annotation more info
involved_in regulation of muscle filament sliding	ISO Inferred from Sequence Orthology more info
involved_in regulation of neuronal synaptic plasticity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in skeletal muscle cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in skeletal muscle satellite cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in skeletal muscle satellite cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in striated muscle contraction	IBA Inferred from Biological aspect of Ancestor more info
involved_in striated muscle contraction	IEA Inferred from Electronic Annotation more info
involved_in striated muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in synaptic vesicle transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in water transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in synapse	IEA Inferred from Electronic Annotation more info
located_in synapse	ISO Inferred from Sequence Orthology more info
located_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in terminal bouton	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: myosin light chain kinase 2, skeletal/cardiac muscle

Names: MLCK2; myosin light chain kinase 2, skeletal muscle; myosin, light polypeptide kinase 2

NP_476557.2

EC 2.7.11.18

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_057209.2 → NP_476557.2 myosin light chain kinase 2, skeletal/cardiac muscle

Status: VALIDATED

Source sequence(s)

JAXUCZ010000003

UniProtKB/Swiss-Prot

P20689

UniProtKB/TrEMBL

A6KHS0, G3V731

Related

ENSRNOP00000011255.2, ENSRNOT00000011255.6

Conserved Domains (1) summary

cl21453
Location:295 → 554

PKc_like; Protein Kinases, catalytic domain