Dgat1 diacylglycerol O-acyltransferase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Dgat1provided by RGD
Official Full Name: diacylglycerol O-acyltransferase 1provided by RGD
Primary source: RGD:628673
See related: Ensembl:ENSRNOG00000028711 AllianceGenome:RGD:628673
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: ARAT; Dgat
Summary: Enables diacylglycerol O-acyltransferase activity; diacylglycerol binding activity; and fatty acid binding activity. Involved in several processes, including insulin secretion; positive regulation of hepatic stellate cell activation; and regulation of lipid metabolic process. Located in intracellular membrane-bounded organelle and plasma membrane. Biomarker of chronic kidney disease and nephrotic syndrome. Human ortholog(s) of this gene implicated in cardiomyopathy and congenital diarrhea 7 with exudative enteropathy. Orthologous to human DGAT1 (diacylglycerol O-acyltransferase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Adrenal (RPKM 224.1), Thymus (RPKM 172.3) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q34

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (110104514..110119091, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (108223860..108235413, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (117566363..117576735, complement)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TAG synthesis and levels of PUFA-TAGs were lowered by the diacylglycerol acyltransferase (DGAT)1 inhibitor, T863. The lipase inhibitor, Atglistatin, increased the levels of TAG in both WT and ATGL-deficient mouse Hepatic stellate cell (HSC). Both Atglistatin and T863 inhibited the induction of activation marker, alpha-smooth muscle actin, in rat HSCs, but not in mouse HSCs.
Title: ATGL and DGAT1 are involved in the turnover of newly synthesized triacylglycerols in hepatic stellate cells.
In both wild-type and DGAT-knockdown hepatocytes, oleate co-treatment significantly reduced PA lipid incorporation and overall phospholipid saturation compared to PA-treated hepatocytes
Title: Knockdown of triglyceride synthesis does not enhance palmitate lipotoxicity or prevent oleate-mediated rescue in rat hepatocytes.
pharmacological DGAT-1 inhibition leads to an increase in intestinal FAO and ketogenesis when dietary fat is available.
Title: Diacylglycerol acyltransferase-1 inhibition enhances intestinal fatty acid oxidation and reduces energy intake in rats.
DGAT1 belongs to the family of oligomeric membrane proteins that adopt a dual membrane topology.
Title: Evidence that diacylglycerol acyltransferase 1 (DGAT1) has dual membrane topology in the endoplasmic reticulum of HepG2 cells.
unilateral DGAT1 overexpression can rescue insulin sensitivity, possibly by increasing DAG and TAG turnover in skeletal muscle
Title: Paradoxical increase in TAG and DAG content parallel the insulin sensitizing effect of unilateral DGAT1 overexpression in rat skeletal muscle.
Propose isoflavonoids with isoprenyl groups as a novel class of DGAT inhibitors.
Title: Diacylglycerol acyltransferase-inhibitory compounds from Erythrina senegalensis.
the acylation of acylglycerols by DGAT1 is important for dietary fat absorption in the intestine
Title: Acylation of acylglycerols by acyl coenzyme A:diacylglycerol acyltransferase 1 (DGAT1). Functional importance of DGAT1 in the intestinal fat absorption.
DGAT2, but not DGAT1, has a role in diet-induced hepatic steatosis and insulin resistance
Title: Suppression of diacylglycerol acyltransferase-2 (DGAT2), but not DGAT1, with antisense oligonucleotides reverses diet-induced hepatic steatosis and insulin resistance.
Nephrotic syndrome results in up-regulation of hepatic DGAT-1 expression and activity, which can potentially contribute to the associated hypertriglyceridemia by enhancing triglyceride synthesis.
Title: Up-regulation of hepatic Acyl CoA: Diacylglycerol acyltransferase-1 (DGAT-1) expression in nephrotic syndrome.
increasing DGAT1, ACAT1, or ACAT2 expression stimulates the assembly and secretion of VLDL from liver cells
Title: Overexpression of human diacylglycerol acyltransferase 1, acyl-coa:cholesterol acyltransferase 1, or acyl-CoA:cholesterol acyltransferase 2 stimulates secretion of apolipoprotein B-containing lipoproteins in McA-RH7777 cells.

Submit: New GeneRIF Correction See all GeneRIFs (13)

General gene information

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Markers

Dgat1 (e-PCR), detects polymorphism
- UniSTS:527311

AA859529 (e-PCR)
- UniSTS:243929

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 2-acylglycerol O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables 2-acylglycerol O-acyltransferase activity	ISO Inferred from Sequence Orthology more info
enables acyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables diacylglycerol O-acyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables diacylglycerol O-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables diacylglycerol O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables diacylglycerol O-acyltransferase activity	ISO Inferred from Sequence Orthology more info
enables diacylglycerol binding	IDA Inferred from Direct Assay more info	PubMed
enables fatty acid binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables retinol O-fatty-acyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in diacylglycerol metabolic process	IEA Inferred from Electronic Annotation more info
involved_in diacylglycerol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in diacylglycerol metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in fatty acid homeostasis	IEA Inferred from Electronic Annotation more info
involved_in fatty acid homeostasis	ISO Inferred from Sequence Orthology more info
involved_in glycerolipid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in insulin secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ketone body metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid storage	IEA Inferred from Electronic Annotation more info
involved_in lipid storage	ISO Inferred from Sequence Orthology more info
involved_in long-chain fatty-acyl-CoA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in long-chain fatty-acyl-CoA metabolic process	ISO Inferred from Sequence Orthology more info
involved_in monoacylglycerol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in monoacylglycerol biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in monoacylglycerol biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of fatty acid oxidation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of hepatic stellate cell activation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of triglyceride biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of eating behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of intestinal lipid absorption	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of vitamin A metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to human chorionic gonadotropin	IEP Inferred from Expression Pattern more info	PubMed
involved_in retinol metabolic process	IEA Inferred from Electronic Annotation more info
involved_in triglyceride biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in triglyceride biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in triglyceride biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within triglyceride biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in triglyceride biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in very-low-density lipoprotein particle assembly	IEA Inferred from Electronic Annotation more info
involved_in very-low-density lipoprotein particle assembly	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: diacylglycerol O-acyltransferase 1

Names: acyl-CoA retinol O-fatty-acyltransferase; acyl-coenzyme A:diacylglycerol acyltransferase 1; diacylglycerol acyltransferase; diglyceride acyltransferase; retinol O-fatty-acyltransferase

NP_445889.2

EC 2.3.1.20

EC 2.3.1.76

XP_063120405.1

EC 2.3.1.20

EC 2.3.1.76

XP_063120406.1

EC 2.3.1.20

EC 2.3.1.76

XP_063120407.1

EC 2.3.1.20

EC 2.3.1.76

XP_063120408.1

EC 2.3.1.20

EC 2.3.1.76

XP_063120409.1

EC 2.3.1.20

EC 2.3.1.76

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053437.2 → NP_445889.2 diacylglycerol O-acyltransferase 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000007

UniProtKB/Swiss-Prot

Q9ERM3

UniProtKB/TrEMBL

A6HSA3, Q793I4, Q8BHI5

Related

ENSRNOP00000035110.4, ENSRNOT00000039795.4

Conserved Domains (1) summary

cl00738
Location:81 → 485

MBOAT; membrane-bound O-acyltransferase family

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

110104514..110119091 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063264335.1 → XP_063120405.1 diacylglycerol O-acyltransferase 1 isoform X1

UniProtKB/Swiss-Prot

Q9ERM3
XM_063264336.1 → XP_063120406.1 diacylglycerol O-acyltransferase 1 isoform X2

UniProtKB/Swiss-Prot

Q9ERM3
XM_063264337.1 → XP_063120407.1 diacylglycerol O-acyltransferase 1 isoform X3

UniProtKB/Swiss-Prot

Q9ERM3
XM_063264339.1 → XP_063120409.1 diacylglycerol O-acyltransferase 1 isoform X5
XM_063264338.1 → XP_063120408.1 diacylglycerol O-acyltransferase 1 isoform X4