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    LEU2 3-isopropylmalate dehydrogenase [ Saccharomyces cerevisiae S288C ]

    Gene ID: 850342, updated on 9-Jun-2024

    Summary

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    Gene symbol
    LEU2
    Gene description
    3-isopropylmalate dehydrogenase
    Primary source
    FungiDB:YCL018W
    Locus tag
    YCL018W
    See related
    SGD:S000000523; AllianceGenome:SGD:S000000523; VEuPathDB:YCL018W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables 3-isopropylmalate dehydrogenase activity. Involved in glyoxylate cycle and leucine biosynthetic process. Located in cytosol. [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See LEU2 in Genome Data Viewer
    Location:
    chromosome: III
    Exon count:
    1
    Sequence:
    Chromosome: III; NC_001135.5 (91324..92418)

    Chromosome III - NC_001135.5Genomic Context describing neighboring genes Neighboring gene gag protein Neighboring gene gag-pol fusion protein Neighboring gene tRNA-Leu Neighboring gene cysteine desulfurase Neighboring gene Dcc1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001135.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Construction of a genomic library of the food spoilage yeast Zygosaccharomyces bailii and isolation of the beta-isopropylmalate dehydrogenase gene (ZbLEU2). Rodrigues F, et al. FEMS Yeast Res, 2001 Apr. PMID 12702464
    2. Molecular cloning and sequence analysis of the Zygosaccharomyces rouxiiLEU2 gene encoding a beta-isopropylmalate dehydrogenase. Sychrová H. Yeast, 2001 Jul. PMID 11447605
    3. Selection of stabilized 3-isopropylmalate dehydrogenase of Saccharomyces cerevisiae using the host-vector system of an extreme thermophile, Thermus thermophilus. Tamakoshi M, et al. Extremophiles, 2001 Feb. PMID 11302498
    4. Isolation by genetic complementation of two differentially expressed genes for beta-isopropylmalate dehydrogenase from Aspergillus niger. Williams BA, et al. Curr Genet, 1996 Sep. PMID 8781173
    5. Leucine biosynthesis in Saccharomyces cerevisiae. Purification and characterization of beta-isopropylmalate dehydrogenase. Hsu YP, et al. J Biol Chem, 1980 Aug 10. PMID 6993484

    See all (68) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables 3-isopropylmalate dehydrogenase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 3-isopropylmalate dehydrogenase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 3-isopropylmalate dehydrogenase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 3-isopropylmalate dehydrogenase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables NAD binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in L-leucine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in L-leucine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in L-leucine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in glyoxylate cycle IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    3-isopropylmalate dehydrogenase
    NP_009911.2
    • Beta-isopropylmalate dehydrogenase (IMDH); catalyzes the third step in the leucine biosynthesis pathway; can additionally catalyze the conversion of beta-ethylmalate into alpha-ketovalerate

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001135.5 Reference assembly

      Range
      91324..92418
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178665.1 → NP_009911.2  TPA: 3-isopropylmalate dehydrogenase [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_009911.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VQZ6, P04173
      UniProtKB/TrEMBL
      A0A6A5Q1A0, A6ZTG8, B5VET6, C7GQK2, C8Z469, G2WA04
      Conserved Domains (1) summary
      TIGR00169
      Location:6 → 359
      leuB; 3-isopropylmalate dehydrogenase
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P04173.4

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