Grm8 glutamate metabotropic receptor 8 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Grm8provided by RGD
Official Full Name: glutamate metabotropic receptor 8provided by RGD
Primary source: RGD:619858
See related: Ensembl:ENSRNOG00000021468 AllianceGenome:RGD:619858
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Glur8; mGlur; Gprc1h; Mglur8; mGluR8b
Summary: Enables group III metabotropic glutamate receptor activity. Involved in detection of mechanical stimulus involved in sensory perception of pain; glutamatergic synaptic transmission; and regulation of synaptic vesicle exocytosis. Located in neuron projection; neuronal cell body; and presynaptic membrane. Is integral component of plasma membrane. Is active in GABA-ergic synapse and glutamatergic synapse. Is integral component of presynaptic active zone membrane. Used to study Parkinsonism; epilepsy; and visual epilepsy. Human ortholog(s) of this gene implicated in attention deficit hyperactivity disorder; autistic disorder; and schizophrenia. Orthologous to human GRM8 (glutamate metabotropic receptor 8). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 78.5) and Muscle (RPKM 6.3) See more
Orthologs: human mouse all
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Genomic context

Location:: 4q22

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	4	NC_086022.1 (56771247..57696951, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	4	NC_051339.1 (55805762..56731690, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	4	NC_005103.4 (54474344..55409526, complement)

Chromosome 4 - NC_086022.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Metabotropic Glutamate Receptor 8 Suppresses M1 Polarization in Microglia by Alleviating Endoplasmic Reticulum Stress and Mitochondrial Dysfunction.
Title: Metabotropic Glutamate Receptor 8 Suppresses M1 Polarization in Microglia by Alleviating Endoplasmic Reticulum Stress and Mitochondrial Dysfunction.
Synaptic organisation and behaviour-dependent activity of mGluR8a-innervated GABAergic trilaminar cells projecting from the hippocampus to the subiculum.
Title: Synaptic organisation and behaviour-dependent activity of mGluR8a-innervated GABAergic trilaminar cells projecting from the hippocampus to the subiculum.
This study shows via knockdown of endogenous MEF2A in primary neuronal culture, that MEF2A is specifically required for Group I metabotropic glutamate receptor (mGluR)-mediated GluA2 internalisation, but does not regulate AMPAR expression or trafficking under basal conditions.
Title: MEF2A regulates mGluR-dependent AMPA receptor trafficking independently of Arc/Arg3.1.
Results show that group III mGluRs negatively modulate TRPA1 activity on cutaneous nociceptors. Furthermore, it is likely that this modulation occurs intracellularly at the level of the cAMP/PKA pathway. This study demonstrates that group III agonists may be effective in the treatment of mechanical hypersensitivity which can develop as a result of inflammation, nerve injury, chemotherapy and other disease states.
Title: Group III mGluR8 negatively modulates TRPA1.
Findings from the present study showed that trigeminal neurons express mGluR1alpha, mGluR2/3 and mGluR8, while SGCs only express mGluR1alpha and mGluR8.
Title: Investigating the expression of metabotropic glutamate receptors in trigeminal ganglion neurons and satellite glial cells: implications for craniofacial pain.
These results suggest that stimulation of mGluR8 inhibits thermoceptive responses and mechanical allodynia.
Title: Dorsal striatum metabotropic glutamate receptor 8 affects nocifensive responses and rostral ventromedial medulla cell activity in neuropathic pain conditions.
These data suggest that mGluR7 in the nucleus tractus solitarius display an inhibitory effect, while mGluR8 displays a facilitatory effect in modulating cardiac nociception
Title: Metabotropic glutamate subtype 7 and 8 receptors oppositely modulate cardiac nociception in the rat nucleus tractus solitarius.
In conclusion, our data identify a mode of presynaptic inhibition which allows mGluR8 to profoundly inhibit vesicle fusion.
Title: Depression of release by mGluR8 alters Ca2+ dependence of release machinery.
Selective activation of mGluR8 with L(+)-2-amino-4-phosphnobytyric acid decreased the probability of glutamate release from the mossy fiber terminals synapsing onto stratum lacunosum-moleculare interneurons.
Title: High affinity group III mGluRs regulate mossy fiber input to CA3 interneurons.
SUMO E3 ligases are expressed in the retina and regulate SUMOylation of the metabotropic glutamate receptor 8b.
Title: SUMO E3 ligases are expressed in the retina and regulate SUMOylation of the metabotropic glutamate receptor 8b.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AU026010 (e-PCR)
- UniSTS:114701

d4mgh15 (e-PCR)
- UniSTS:121919

d4mit9 (e-PCR)
- UniSTS:121933

D4Got33 (e-PCR)
- UniSTS:112253

Gprc1h (e-PCR), detects polymorphism
- UniSTS:143265

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables G protein-coupled receptor activity	IEA Inferred from Electronic Annotation more info
enables G protein-coupled receptor activity	ISO Inferred from Sequence Orthology more info
enables G protein-coupled receptor activity	ISS Inferred from Sequence or Structural Similarity more info
enables glutamate receptor activity	IEA Inferred from Electronic Annotation more info
enables glutamate receptor activity	ISO Inferred from Sequence Orthology more info
enables glutamate receptor activity	ISS Inferred from Sequence or Structural Similarity more info
enables group III metabotropic glutamate receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables group III metabotropic glutamate receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables group III metabotropic glutamate receptor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in G protein-coupled glutamate receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in adenylate cyclase-inhibiting G protein-coupled glutamate receptor signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within adenylate cyclase-inhibiting G protein-coupled glutamate receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in adenylate cyclase-inhibiting G protein-coupled glutamate receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in adenylate cyclase-inhibiting G protein-coupled glutamate receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in adenylate cyclase-inhibiting G protein-coupled glutamate receptor signaling pathway	TAS Traceable Author Statement more info	PubMed
involved_in adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in chemical synaptic transmission	NAS Non-traceable Author Statement more info	PubMed
involved_in detection of mechanical stimulus involved in sensory perception of pain	IDA Inferred from Direct Assay more info	PubMed
involved_in presynaptic modulation of chemical synaptic transmission	EXP Inferred from Experiment more info	PubMed
involved_in presynaptic modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of neurotransmitter secretion	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of synaptic transmission, glutamatergic	IBA Inferred from Biological aspect of Ancestor more info
involved_in sensory perception of pain	TAS Traceable Author Statement more info	PubMed
involved_in synaptic transmission, glutamatergic	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in GABA-ergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in GABA-ergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in postsynaptic membrane	TAS Traceable Author Statement more info	PubMed
is_active_in presynaptic active zone membrane	IDA Inferred from Direct Assay more info	PubMed
located_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: metabotropic glutamate receptor 8

Names: G protein-coupled receptor, family C, group 1, member H; glutamate receptor, metabotropic 8; metabotropic glutamate receptor subtype 8b

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_022202.1 → NP_071538.1 metabotropic glutamate receptor 8 precursor

See identical proteins and their annotated locations for NP_071538.1

Status: PROVISIONAL

Source sequence(s)

U63288

UniProtKB/Swiss-Prot

P70579

Conserved Domains (4) summary

cd06376
Location:44 → 504

PBP1_mGluR_groupIII; Ligand-binding domain of the group III metabotropic glutamate receptor

pfam00003
Location:595 → 840

7tm_3; 7 transmembrane sweet-taste receptor of 3 GCPR

pfam01094
Location:74 → 478

ANF_receptor; Receptor family ligand binding region

pfam07562
Location:514 → 560

NCD3G; Nine Cysteines Domain of family 3 GPCR

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086022.1 Reference GRCr8

Range

56771247..57696951 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017592855.3 → XP_017448344.1 metabotropic glutamate receptor 8 isoform X1

UniProtKB/TrEMBL

A6IEA3, F1LRA6

Conserved Domains (3) summary

cd15454
Location:582 → 892

7tmC_mGluR8; metabotropic glutamate receptor 8 in group 3, member of the class C family of seven-transmembrane G protein-coupled receptors

cd06376
Location:40 → 504

PBP1_mGluR_groupIII; ligand-binding domain of the group III metabotropic glutamate receptor

pfam07562
Location:512 → 562

NCD3G; Nine Cysteines Domain of family 3 GPCR
XM_017592857.3 → XP_017448346.1 metabotropic glutamate receptor 8 isoform X2

UniProtKB/TrEMBL

F1LRA6
XM_017592856.3 → XP_017448345.1 metabotropic glutamate receptor 8 isoform X1

UniProtKB/TrEMBL

A6IEA3, F1LRA6

Related

ENSRNOP00000035953.5

Conserved Domains (3) summary

cd15454
Location:582 → 892

7tmC_mGluR8; metabotropic glutamate receptor 8 in group 3, member of the class C family of seven-transmembrane G protein-coupled receptors

cd06376
Location:40 → 504

PBP1_mGluR_groupIII; ligand-binding domain of the group III metabotropic glutamate receptor

pfam07562
Location:512 → 562

NCD3G; Nine Cysteines Domain of family 3 GPCR
XM_017592859.3 → XP_017448348.1 metabotropic glutamate receptor 8 isoform X1

UniProtKB/TrEMBL

A6IEA3, F1LRA6

Conserved Domains (3) summary

cd15454
Location:582 → 892

7tmC_mGluR8; metabotropic glutamate receptor 8 in group 3, member of the class C family of seven-transmembrane G protein-coupled receptors

cd06376
Location:40 → 504

PBP1_mGluR_groupIII; ligand-binding domain of the group III metabotropic glutamate receptor

pfam07562
Location:512 → 562

NCD3G; Nine Cysteines Domain of family 3 GPCR