Ucma upper zone of growth plate and cartilage matrix associated [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ucmaprovided by MGI
Official Full Name: upper zone of growth plate and cartilage matrix associatedprovided by MGI
Primary source: MGI:MGI:1915777
See related: Ensembl:ENSMUSG00000026668 AllianceGenome:MGI:1915777
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Grp; 1110017I16Rik
Summary: This gene encodes chondrocyte-specific, highly charged proteins that are abundantly expressed during the early stages of chondrogenesis. The encoded protein undergoes proteolytic processing to generate a mature protein that is secreted into the extracellular matrix. The glutamic acid residues in the encoded protein undergo gamma carboxylation in a vitamin K-dependent manner. Despite the implied role in calcification and ossification, mice lacking the encoded protein do not display significant defects in the skeletal development. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo a similar proteolytic processing to generate mature proteins. [provided by RefSeq, Aug 2015]
Expression: Biased expression in cerebellum adult (RPKM 2.9), limb E14.5 (RPKM 1.9) and 7 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 A1; 2 3.11 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (4980933..4990559)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (4976122..4985748)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Unique cartilage matrix-associated protein inhibits the migratory and invasive potential of triple-negative breast cancer.
Title: Unique cartilage matrix-associated protein inhibits the migratory and invasive potential of triple-negative breast cancer.
Downstream target genes of Ucma were found in osteoblasts by microarray analysis. 45 genes including Fbn2 were dependent on Ucma overexpression or knockdown. Fbn2 expression was proportional to Ucma expression in osteoblasts.
Title: Unique cartilage matrix-associated protein regulates fibrillin-2 expression and directly interacts with fibrillin-2 protein independent of calcium binding.
Study using Ucma/GRP-/- and ApoE-/- mice showed for the first-time evidence of a direct interaction between Ucma/GRP and Bmp-2. These results demonstrate an important role of Ucma/GRP in regulating osteo/chondrogenic differentiation and phosphate-induced mineralisation of vascular smooth muscle cells.
Title: Ucma/GRP inhibits phosphate-induced vascular smooth muscle cell calcification via SMAD-dependent BMP signalling.
Upper zone of growth plate and cartilage matrix associated protein (Ucma) physically interacted with a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) protein (ADAMTS5) and blocked its aggrecanase activity in chondrocyte cultures.
Title: Upper zone of growth plate and cartilage matrix associated protein protects cartilage during inflammatory arthritis.
Data suggest that a novel splice variant of GRP is expressed in cartilage and other tissues (i.e. GRP-F7); GRP-F1 is predominant splice variant expressed in adult tissues.
Title: Insights into the association of Gla-rich protein and osteoarthritis, novel splice variants and γ-carboxylation status.
Considering that skeletal development in Ucma-deficient mice is not significantly impaired, protein expression in adult cartilage indicates that Ucma might be involved in skeletal homeostasis and in the mechanical properties of the skeleton
Title: Ucma is not necessary for normal development of the mouse skeleton.
Four alternative splicing variants derived from the mouse Ucma/GRP gene in chondrocytes were identified and their expression and subcellular localization during chondrogenesis was determined.
Title: Identification of four alternatively spliced transcripts of the Ucma/GRP gene, encoding a new Gla-containing protein.
The present data strongly suggest an important function of Ucma in the early phase of chondrocyte differentiation.
Title: Ucma--A novel secreted factor represents a highly specific marker for distal chondrocytes.
Ucma may be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells
Title: Ucma, a novel secreted cartilage-specific protein with implications in osteogenesis.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (1) 1 citation

General gene information

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Markers

1110017I16Rik (e-PCR), detects polymorphism
- UniSTS:508528

AW121955 (e-PCR)
- UniSTS:178559

NoName (e-PCR)
- UniSTS:493021

D2Mit354 (e-PCR), detects polymorphism
- UniSTS:129247

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables BMP binding	ISO Inferred from Sequence Orthology more info	PubMed

Process	Evidence Code	Pubs
involved_in embryonic skeletal system development	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of SMAD protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of osteoblast differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of osteoblast differentiation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of osteoblast differentiation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
is_active_in extracellular matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular matrix	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in extracellular region	TAS Traceable Author Statement more info

General protein information

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Preferred Names: unique cartilage matrix-associated protein

Names: Gla-rich protein; upper zone of growth plate and cartilage matrix associated protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001113558.2 → NP_001107030.1 unique cartilage matrix-associated protein isoform b preproprotein

See identical proteins and their annotated locations for NP_001107030.1

Status: REVIEWED

Description

Transcript Variant: This variant (2, also known as Ucma/GRP-F1) contains an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (b) is longer than isoform a.

Source sequence(s)

BY590633, EU368184

Consensus CDS

CCDS50489.1

UniProtKB/Swiss-Prot

C9W8R6, C9W8R7, C9W8R8, C9W8R9, Q14BU0, Q9D1A9

Related

ENSMUSP00000110662.3, ENSMUST00000115010.9

Conserved Domains (1) summary

pfam17085
Location:1 → 134

UCMA; Unique cartilage matrix associated protein
NM_001165932.1 → NP_001159404.1 unique cartilage matrix-associated protein isoform c precursor

See identical proteins and their annotated locations for NP_001159404.1

Status: REVIEWED

Description

Transcript Variant: This variant (3, also known as Ucma/GRP-F3) contains an alternate in-frame exon in the 5' coding region and lacks an in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. This isoform (c) may undergo proteolytic processing similar to isoform b.

Source sequence(s)

BE648935, BY590633, EU368184

Consensus CDS

CCDS50490.1

UniProtKB/Swiss-Prot

Q14BU0

Related

ENSMUSP00000126371.2, ENSMUST00000167607.8

Conserved Domains (1) summary

pfam17085
Location:1 → 101

UCMA; Unique cartilage matrix associated protein
NM_001311208.1 → NP_001298137.1 unique cartilage matrix-associated protein isoform d precursor

Status: REVIEWED

Description

Transcript Variant: This variant (4, also known as Ucma/GRP-F4) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (d) is shorter than isoform a. This isoform (d) may undergo proteolytic processing similar to isoform b.

Source sequence(s)

BY590633, EU368184, FJ217398

Consensus CDS

CCDS84467.1

UniProtKB/Swiss-Prot

Q14BU0

Related

ENSMUSP00000141304.2, ENSMUST00000195688.2

Conserved Domains (1) summary

pfam17085
Location:22 → 79

UCMA; Unique cartilage matrix associated protein
NM_026754.3 → NP_081030.1 unique cartilage matrix-associated protein isoform a

See identical proteins and their annotated locations for NP_081030.1

Status: REVIEWED

Description

Transcript Variant: This variant (1, also known as Ucma/GRP-F2) encodes isoform a. This isoform (a) may undergo proteolytic processing similar to isoform b.

Source sequence(s)

AK003750, BY590633, EU368184

Consensus CDS

CCDS15661.1

UniProtKB/Swiss-Prot

Q14BU0

Related

ENSMUSP00000027978.2, ENSMUST00000027978.7

Conserved Domains (1) summary

pfam17085
Location:22 → 112

UCMA; Unique cartilage matrix associated protein