Lima1 LIM domain and actin binding 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Lima1provided by MGI
Official Full Name: LIM domain and actin binding 1provided by MGI
Primary source: MGI:MGI:1920992
See related: Ensembl:ENSMUSG00000023022 AllianceGenome:MGI:1920992
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Eplin
Summary: Enables actin filament binding activity. Involved in several processes, including cholesterol homeostasis; intestinal cholesterol absorption; and ruffle organization. Located in actin cytoskeleton; brush border membrane; and ruffle. Is expressed in several structures, including alimentary system; brain; genitourinary system; immune system; and respiratory system. Orthologous to human LIMA1 (LIM domain and actin binding 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in bladder adult (RPKM 44.8), colon adult (RPKM 23.3) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 F1; 15 56.13 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (99676351..99773292, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (99778468..99875477, complement)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism.
Title: Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism.
this study identifies LIMA1 as a key protein regulating intestinal cholesterol absorption.
Title: A LIMA1 variant promotes low plasma LDL cholesterol and decreases intestinal cholesterol absorption.
findings suggest that PINCH-1 regulates integrin-mediated adhesion of keratinocytes through the interactions with ILK as well as EPLIN.
Title: The focal adhesion protein PINCH-1 associates with EPLIN at integrin adhesion sites.
Eplin-alpha transcription is regulated by actin-MAL-SRF signalling.
Title: Epithelial Protein Lost in Neoplasm alpha (Eplin-alpha) is transcriptionally regulated by G-actin and MAL/MRTF coactivators.
EPLIN functions to link the cadherin-catenin complex to F-actin and simultaneously stabilizes this population of actin fibers, resulting in the establishment of the adhesion
Title: EPLIN mediates linkage of the cadherin catenin complex to F-actin and stabilizes the circumferential actin belt.
ERK-mediated phosphorylation of EPLIN contributes to actin filament reorganization and enhanced cell motility.
Title: Extracellular signal-regulated kinase/mitogen-activated protein kinase regulates actin organization and cell motility by phosphorylating the actin cross-linking protein EPLIN.
EPLIN may function in growth control by associating with and regulating the actin cytoskeleton
Title: Inhibition of anchorage-independent growth of transformed NIH3T3 cells by epithelial protein lost in neoplasm (EPLIN) requires localization of EPLIN to actin cytoskeleton.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation
Endonuclease-mediated (2)
Gene trapped (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Eplin-pending (e-PCR), detects polymorphism
- UniSTS:225830

Eplin-pending (e-PCR), detects polymorphism
- UniSTS:225831

RH127071 (e-PCR)
- UniSTS:167155

RH126358 (e-PCR)
- UniSTS:163066

Clone Names

D15Ertd366e, 1110021C24Rik, 3526402A12Rik

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin filament binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables actin filament binding	ISO Inferred from Sequence Orthology more info
enables actin monomer binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin filament bundle assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament bundle assembly	ISO Inferred from Sequence Orthology more info
involved_in cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cholesterol homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cholesterol metabolic process	IEA Inferred from Electronic Annotation more info
involved_in intestinal cholesterol absorption	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of actin filament depolymerization	ISO Inferred from Sequence Orthology more info
involved_in ruffle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ruffle organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within steroid metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in brush border	IDA Inferred from Direct Assay more info	PubMed
located_in brush border membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cleavage furrow	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in focal adhesion	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in ruffle	IDA Inferred from Direct Assay more info	PubMed
located_in stress fiber	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: LIM domain and actin-binding protein 1

Names: epithelial protein lost in neoplasm

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001113545.2 → NP_001107017.1 LIM domain and actin-binding protein 1 isoform a

See identical proteins and their annotated locations for NP_001107017.1

Status: VALIDATED

Description

Transcript Variant: This variant (a) encodes the longer isoform (a).

Source sequence(s)

AC134548, AC138177

Consensus CDS

CCDS49729.1

UniProtKB/Swiss-Prot

Q8K2H0, Q9ERG0, Q9ERG1

UniProtKB/TrEMBL

Q8C7S2

Related

ENSMUSP00000073371.4, ENSMUST00000073691.5

Conserved Domains (1) summary

cd09485
Location:388 → 440

LIM_Eplin_alpha_beta; The Lim domain of Epithelial Protein Lost in Neoplasm (Eplin)
NM_023063.5 → NP_075550.2 LIM domain and actin-binding protein 1 isoform b

See identical proteins and their annotated locations for NP_075550.2

Status: VALIDATED

Description

Transcript Variant: This variant (b) uses an alternate 5'-terminal exon, lacks part of the 5' coding region, and initiates translation from a downstream start codon, compared to variant a. The encoded isoform (b) has a shorter N-terminus, compared to isoform a.

Source sequence(s)

AC134548

Consensus CDS

CCDS37206.1

UniProtKB/TrEMBL

Q8BT15, Q8CD09

Related

ENSMUSP00000104652.3, ENSMUST00000109024.9

Conserved Domains (1) summary

cd09485
Location:228 → 280

LIM_Eplin_alpha_beta; The Lim domain of Epithelial Protein Lost in Neoplasm (Eplin)

RNA

NR_177985.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC134548, AC138177

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

99676351..99773292 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006521245.2 → XP_006521308.1 LIM domain and actin-binding protein 1 isoform X1

Conserved Domains (1) summary

cd09485
Location:101 → 153

LIM_Eplin_alpha_beta; The Lim domain of Epithelial Protein Lost in Neoplasm (Eplin)