Dbt dihydrolipoamide branched chain transacylase E2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Dbtprovided by RGD
Official Full Name: dihydrolipoamide branched chain transacylase E2provided by RGD
Primary source: RGD:68403
See related: Ensembl:ENSRNOG00000015029 AllianceGenome:RGD:68403
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Predicted to enable acetyltransferase activity; lipoic acid binding activity; and ubiquitin protein ligase binding activity. Predicted to be located in cytosol; microtubule cytoskeleton; and mitochondrial nucleoid. Predicted to be active in mitochondrion. Human ortholog(s) of this gene implicated in maple syrup urine disease. Orthologous to human DBT (dihydrolipoamide branched chain transacylase E2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Kidney (RPKM 287.4), Heart (RPKM 253.9) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 2q42

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	2	NC_086020.1 (207166660..207195528)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	2	NC_051337.1 (204481744..204510612)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	2	NC_005101.4 (219563783..219592651)

Chromosome 2 - NC_086020.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

GDB:4585461 (e-PCR)
- UniSTS:74520

AI412898 (e-PCR)
- UniSTS:249951

RH131391 (e-PCR)
- UniSTS:214674

D2Wox8 (e-PCR)
- UniSTS:117955

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables acetyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables branched-chain alpha-keto acid dehydrogenase activity	IEA Inferred from Electronic Annotation more info
contributes_to branched-chain alpha-keto acid dehydrogenase activity	ISO Inferred from Sequence Orthology more info
enables lipoic acid binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in biological_process	ND No biological Data available more info
involved_in branched-chain amino acid catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in microtubule cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in mitochondrial nucleoid	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
part_of oxidoreductase complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial

NP_445764.1

EC 2.3.1.168

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053312.1 → NP_445764.1 lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial

See identical proteins and their annotated locations for NP_445764.1

Status: VALIDATED

Source sequence(s)

AA955113, AB047915, BC101904, BF547307, BM391990, CK356543

UniProtKB/TrEMBL

A0A8I6A4P4, B2GV15, F7EMY8

Related

ENSRNOP00000020267.4, ENSRNOT00000020267.7

Conserved Domains (4) summary

cd06849
Location:65 → 138

lipoyl_domain; Lipoyl domain of the dihydrolipoyl acyltransferase component (E2) of 2-oxo acid dehydrogenases. 2-oxo acid dehydrogenase multienzyme complexes, like pyruvate dehydrogenase (PDH), 2-oxoglutarate dehydrogenase (OGDH) and branched-chain 2-oxo acid ...

PLN02528
Location:67 → 482

PLN02528; 2-oxoisovalerate dehydrogenase E2 component

pfam00198
Location:267 → 479

2-oxoacid_dh; 2-oxoacid dehydrogenases acyltransferase (catalytic domain)

pfam02817
Location:172 → 205

E3_binding; e3 binding domain