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    Rap1gap Rap1 GTPase-activating protein [ Rattus norvegicus (Norway rat) ]

    Gene ID: 313644, updated on 13-Apr-2024

    Summary

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    Official Symbol
    Rap1gapprovided by RGD
    Official Full Name
    Rap1 GTPase-activating proteinprovided by RGD
    Primary source
    RGD:1309908
    See related
    Ensembl:ENSRNOG00000013825 AllianceGenome:RGD:1309908
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    Rap1ga1
    Summary
    Predicted to enable GTPase activator activity; protein homodimerization activity; and small GTPase binding activity. Involved in several processes, including cellular response to glial cell derived neurotrophic factor; negative regulation of GTP binding activity; and negative regulation of thyroid gland epithelial cell proliferation. Located in several cellular components, including dendrite; early endosome; and neuronal cell body. Orthologous to human RAP1GAP (RAP1 GTPase activating protein). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Biased expression in Brain (RPKM 252.8), Kidney (RPKM 125.7) and 4 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    Location:
    5q36
    Exon count:
    31
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 5 NC_086023.1 (155157511..155222622)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 5 NC_051340.1 (149873987..149939254)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 5 NC_005104.4 (156008868..156074350)

    Chromosome 5 - NC_086023.1Genomic Context describing neighboring genes Neighboring gene interferon induced transmembrane protein 2, pseudogene 3 Neighboring gene ubiquitin specific peptidase 48 Neighboring gene alkaline phosphatase, biomineralization associated Neighboring gene uncharacterized LOC134487019 Neighboring gene U4 spliceosomal RNA

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. Endothelial cAMP deactivates ischemia-reperfusion-induced microvascular hyperpermeability via Rap1-mediated mechanisms. Korayem AH, et al. Am J Physiol Heart Circ Physiol, 2017 Jul 1. PMID 28476918, Free PMC Article
    2. Regulation of cortical dendrite development by Rap1 signaling. Chen Y, et al. Mol Cell Neurosci, 2005 Feb. PMID 15691704
    3. Rutin treats myocardial damage caused by pirarubicin via regulating miR-22-5p-regulated RAP1/ERK signaling pathway. Qin M, et al. J Biochem Mol Toxicol, 2021 Jan. PMID 32864822
    4. Enhanced Rap1 activation and insulin secretagogue properties of an acetoxymethyl ester of an Epac-selective cyclic AMP analog in rat INS-1 cells: studies with 8-pCPT-2'-O-Me-cAMP-AM. Chepurny OG, et al. J Biol Chem, 2009 Apr 17. PMID 19244230, Free PMC Article
    5. Down-regulation of Rap1GAP via promoter hypermethylation promotes melanoma cell proliferation, survival, and migration. Zheng H, et al. Cancer Res, 2009 Jan 15. PMID 19147557

    See all (33) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Downregulating miR-184 relieves calcium oxalate crystal-mediated renal cell damage via activating the Rap1 signaling pathway.
      Title: Downregulating miR-184 relieves calcium oxalate crystal-mediated renal cell damage via activating the Rap1 signaling pathway.
    2. Rutin treats myocardial damage caused by pirarubicin via regulating miR-22-5p-regulated RAP1/ERK signaling pathway.
      Title: Rutin treats myocardial damage caused by pirarubicin via regulating miR-22-5p-regulated RAP1/ERK signaling pathway.
    3. the present study shows that down-regulated miR-182-5p promotes the proliferation and differentiation of osteoblasts in Osteoporosis (OP) rats through Rap1/MAPK signaling pathway activation by up-regulating ADCY6, which may represent a novel target for OP treatment
      Title: Decreased microRNA-182-5p helps alendronate promote osteoblast proliferation and differentiation in osteoporosis via the Rap1/MAPK pathway.
    4. Here, we demonstrate that 1) stimulation of cAMP production deactivates ischemia-reperfusion-induced hyperpermeability in muscle and endothelial cells; 2) VEGF mRNA expression is not enhanced by brief ischemia, suggesting that VEGF mechanisms do not activate immediate postischemic hyperpermeability; and 3) deactivation mechanisms operate via cAMP-exchange protein activated by cAMP 1-Rap1 to restore integrity of the endot
      Title: Endothelial cAMP deactivates ischemia-reperfusion-induced microvascular hyperpermeability via Rap1-mediated mechanisms.
    5. results have shown a novel role of PLCepsilon in the maintenance of endothelial barrier function, via its CDC25 GEF domain and lipase activity, and subsequent up-regulation of Rap1 activity
      Title: Phospholipase Cε Modulates Rap1 Activity and the Endothelial Barrier.
    6. Rap1GAP negatively regulated GNDF-induced ERK activation and neurite outgrowth.
      Title: Rap1GAP interacts with RET and suppresses GDNF-induced neurite outgrowth.
    7. Data suggest that cAMP-dependent PACAP-induced GFAP expression during astrocytogenesis involves the coordinated activation of both Ras and Rap1, but activation of either one of them in isolation is not sufficient to trigger this response.
      Title: Pituitary adenylate cyclase-activating polypeptide stimulates glial fibrillary acidic protein gene expression in cortical precursor cells by activating Ras and Rap1.
    8. Disruption of the DEP-dependent subcellular targeting of Epac abolished cAMP-Epac-mediated Rap1 activation and thyroid-stimulating hormone-mediated cell proliferation
      Title: Epac, in synergy with cAMP-dependent protein kinase (PKA), is required for cAMP-mediated mitogenesis.
    9. a model in which activation of G(z) results in recruitment of Rap1GAP to the membrane where it can effectively down-regulate Rap1 signaling (Rap1)(Rap1GAP)
      Title: Activation of Gz attenuates Rap1-mediated differentiation of PC12 cells.
    10. Rap1 and MEKK2 are critical upstream signaling molecules mediating BDNF stimulation of ERK5 in central nervous system neurons
      Title: Brain-derived neurotrophic factor activates ERK5 in cortical neurons via a Rap1-MEKK2 signaling cascade.
    Submit: New GeneRIF Correction See all GeneRIFs (20)

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables GTPase activator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables GTPase activator activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables small GTPase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of cell-cell adhesion ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in cellular response to glial cell derived neurotrophic factor IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of establishment of localization in cell ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of microvillus assembly ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of neuron differentiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of thyroid gland epithelial cell proliferation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of phagocytosis ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within positive regulation of cell-cell adhesion ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within positive regulation of phagocytosis ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of small GTPase mediated signal transduction IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in axon IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in dendrite IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in early endosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in neuronal cell body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    rap1 GTPase-activating protein 1

    NCBI Reference Sequences (RefSeq)

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    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001100713.2 → NP_001094183.1  rap1 GTPase-activating protein 1 isoform 1

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000005
      UniProtKB/TrEMBL
      A6ITE6, F1LV89
      Related
      ENSRNOP00000048749.5, ENSRNOT00000046802.6
      Conserved Domains (2) summary
      smart00390
      Location:61 → 81
      GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
      pfam02145
      Location:275 → 454
      Rap_GAP; Rap/ran-GAP

    2. NM_001415932.1 → NP_001402861.1  rap1 GTPase-activating protein 1 isoform 2

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000005
      UniProtKB/TrEMBL
      A6ITE4

    3. NM_001415933.1 → NP_001402862.1  rap1 GTPase-activating protein 1 isoform 3

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000005

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086023.1 Reference GRCr8

      Range
      155157511..155222622
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_063287788.1 → XP_063143858.1  rap1 GTPase-activating protein 1 isoform X16

    2. XM_017593413.2 → XP_017448902.1  rap1 GTPase-activating protein 1 isoform X14

      UniProtKB/TrEMBL
      A6ITE6

    3. XM_063287787.1 → XP_063143857.1  rap1 GTPase-activating protein 1 isoform X15

    4. XM_063287779.1 → XP_063143849.1  rap1 GTPase-activating protein 1 isoform X3

    5. XM_017593409.3 → XP_017448898.1  rap1 GTPase-activating protein 1 isoform X5

      UniProtKB/TrEMBL
      A6ITE6
      Related
      ENSRNOP00000079431.1

    6. XM_063287784.1 → XP_063143854.1  rap1 GTPase-activating protein 1 isoform X11

    7. XM_063287778.1 → XP_063143848.1  rap1 GTPase-activating protein 1 isoform X2

    8. XM_063287780.1 → XP_063143850.1  rap1 GTPase-activating protein 1 isoform X4

    9. XM_063287782.1 → XP_063143852.1  rap1 GTPase-activating protein 1 isoform X8

    10. XM_063287783.1 → XP_063143853.1  rap1 GTPase-activating protein 1 isoform X10

    11. XM_063287785.1 → XP_063143855.1  rap1 GTPase-activating protein 1 isoform X12

    12. XM_063287786.1 → XP_063143856.1  rap1 GTPase-activating protein 1 isoform X14

    13. XM_063287777.1 → XP_063143847.1  rap1 GTPase-activating protein 1 isoform X1

    14. XM_017593410.3 → XP_017448899.1  rap1 GTPase-activating protein 1 isoform X7

      UniProtKB/TrEMBL
      A6ITE6

    15. XM_063287781.1 → XP_063143851.1  rap1 GTPase-activating protein 1 isoform X6

    16. XM_017593412.3 → XP_017448901.1  rap1 GTPase-activating protein 1 isoform X9

      UniProtKB/TrEMBL
      A6ITE6

    17. XM_039110059.2 → XP_038965987.1  rap1 GTPase-activating protein 1 isoform X13

      UniProtKB/TrEMBL
      A0A8I6AU92, A6ITE6
      Related
      ENSRNOP00000097348.1
      Conserved Domains (2) summary
      smart00390
      Location:28 → 48
      GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
      pfam02145
      Location:242 → 421
      Rap_GAP; Rap/ran-GAP

    RNA

    1. XR_354337.3 RNA Sequence

    2. XR_354338.3 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version

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