Mylk3 myosin light chain kinase 3 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Mylk3provided by RGD
Official Full Name: myosin light chain kinase 3provided by RGD
Primary source: RGD:1305801
See related: Ensembl:ENSRNOG00000017546 AllianceGenome:RGD:1305801
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: MLCK; RGD1305801
Summary: Enables calmodulin-dependent protein kinase activity and myosin light chain kinase activity. Involved in myofibril assembly; positive regulation of sarcomere organization; and protein phosphorylation. Located in cytosol. Orthologous to human MYLK3 (myosin light chain kinase 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward (RPKM 1854.5) See more
Orthologs: human mouse all
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Genomic context

Location:: 19q11

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	19	NC_086037.1 (37858298..37916805)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	19	NC_051354.1 (21685085..21743587)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	19	NC_005118.4 (27388163..27442451)

Chromosome 19 - NC_086037.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We found that a rounded shape with high cortical tension depends on MLCK and ROCK, whereas in cells with an elongated shape, MLCK is the primary protein responsible for cell spreading. Further, in cells with a slow and directionally persistent motility, MLCK predominates, while rapid and erratic movement is ROCK-dependent
Title: The shift in GH3 cell shape and cell motility is dependent on MLCK and ROCK.
In conclusion, in addition to MLCK, CaMKII phosphorylates RLC in cardiomyocytes.
Title: CaMKII in addition to MLCK contributes to phosphorylation of regulatory light chain in cardiomyocytes.
Ischemia/reperfusion-activated MMP-2 leads to the degradation of cardiac MLCK.
Title: Degradation of cardiac myosin light chain kinase by matrix metalloproteinase-2 contributes to myocardial contractile dysfunction during ischemia/reperfusion.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calmodulin-dependent protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
NOT enables calmodulin-dependent protein kinase activity	ISO Inferred from Sequence Orthology more info
enables calmodulin-dependent protein kinase activity	ISO Inferred from Sequence Orthology more info
enables myosin light chain kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables myosin light chain kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables myosin light chain kinase activity	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cardiac myofibril assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in cardiac myofibril assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to interleukin-1	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of sarcomere organization	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of vascular permeability involved in acute inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in sarcomere organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in sarcomere organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sarcomere organization	ISO Inferred from Sequence Orthology more info
involved_in sarcomerogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
colocalizes_with actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: myosin light chain kinase 3

Names: cardiac-MLCK; cardiac-MyBP-C-associated Ca/CaM kinase; putative myosin light chain kinase 3

NP_001104280.1

EC 2.7.11.18

XP_006255417.1

EC 2.7.11.18

XP_038953507.1

EC 2.7.11.18

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001110810.1 → NP_001104280.1 myosin light chain kinase 3

See identical proteins and their annotated locations for NP_001104280.1

Status: PROVISIONAL

Source sequence(s)

EU236721

UniProtKB/Swiss-Prot

A8WE96, E9PT87

Related

ENSRNOP00000023657.5, ENSRNOT00000023657.7

Conserved Domains (2) summary

smart00220
Location:485 → 737

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14192
Location:477 → 737

STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086037.1 Reference GRCr8

Range

37858298..37916805

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039097579.2 → XP_038953507.1 myosin light chain kinase 3 isoform X2

Conserved Domains (1) summary

cd14192
Location:198 → 458

STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3
XM_006255355.4 → XP_006255417.1 myosin light chain kinase 3 isoform X1

UniProtKB/TrEMBL

A0A8I5ZYT9

Related

ENSRNOP00000083206.1

Conserved Domains (2) summary

smart00220
Location:352 → 604

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14192
Location:344 → 604

STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3