Rgs14 regulator of G-protein signaling 14 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Rgs14provided by RGD
Official Full Name: regulator of G-protein signaling 14provided by RGD
Primary source: RGD:620003
See related: Ensembl:ENSRNOG00000015616 AllianceGenome:RGD:620003
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables several functions, including G-protein alpha-subunit binding activity; GTPase regulator activity; and signaling receptor complex adaptor activity. Involved in several processes, including platelet-derived growth factor receptor signaling pathway; regulation of signal transduction; and visual learning. Located in several cellular components, including dendrite; microtubule cytoskeleton; and nucleus. Human ortholog(s) of this gene implicated in nephrolithiasis. Orthologous to human RGS14 (regulator of G protein signaling 14). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Thymus (RPKM 184.2), Spleen (RPKM 69.0) and 3 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 17p14

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	17	NC_086035.1 (9254112..9268233, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	17	NC_051352.1 (9248982..9263104, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	17	NC_005116.4 (9777925..9792007, complement)

Chromosome 17 - NC_086035.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

findings describe a novel mechanism of negative regulation of RGS14 functions, specifically interactions with active Galphai and nuclear import, while leaving the function of other RGS14 domains intact
Title: 14-3-3γ binds regulator of G protein signaling 14 (RGS14) at distinct sites to inhibit the RGS14:Gα(i)-AlF(4)(-) signaling complex and RGS14 nuclear localization.
Results show that endogenous RGS14 localizes to subcellular compartments not previously recognized in studies of recombinant RGS14 in unnatural host cells. The two most surprising observations were that RGS14 is not enriched at the plasma membrane, and that it is found within chromatin-rich regions of the nucleus in close proximity with active RNA Pol-II.
Title: Endogenous RGS14 is a cytoplasmic-nuclear shuttling protein that localizes to juxtanuclear membranes and chromatin-rich regions of the nucleus.
Inactive Galpha(i1)-GDP enhances the affinity of RGS14 for H-Ras-GTP in live cells, resulting in a ternary signaling complex that is further regulated by G protein-coupled receptors.
Title: Assembly and function of the regulator of G protein signaling 14 (RGS14)·H-Ras signaling complex in live cells are regulated by Gαi1 and Gαi-linked G protein-coupled receptors.
RGS14 can form complexes with GPCRs in cells that are dependent on Galpha(i/o) and these RGS14.Galpha(i1).GPCR complexes may be substrates for other signaling partners such as Ric-8A
Title: G protein-coupled receptors and resistance to inhibitors of cholinesterase-8A (Ric-8A) both regulate the regulator of g protein signaling 14 RGS14·Gαi1 complex in live cells.
RGS proteins with with GTPase-activating protein (GAP) activity affect both stress resistance and longevity in several species.
Title: Regulation of longevity by regulator of G-protein signaling protein, Loco.
RGS14 facilitates the formation of a selective Ras.GTP-Raf-MEK-ERK multiprotein complex to promote sustained ERK activation and regulate H-Ras-dependent neuritogenesis.
Title: Regulator of G-protein signaling 14 (RGS14) is a selective H-Ras effector.
Structural determinants for GoLoco-induced inhibition of nucleotide release by Galpha subunits
Title: Structural determinants for GoLoco-induced inhibition of nucleotide release by Galpha subunits.
Substituting protein kinase A phosphorylation site Thr494 with either aspartate or glutamate to mimic phosphorylation enhances RGS14 inhibition of nucleotide exchange activity on G alpha(i) subunits almost 3-fold.
Title: Phosphorylation of RGS14 by protein kinase A potentiates its activity toward G alpha i.

Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables G-protein alpha-subunit binding	IEA Inferred from Electronic Annotation more info
enables G-protein alpha-subunit binding	IPI Inferred from Physical Interaction more info	PubMed
enables GDP-dissociation inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables GDP-dissociation inhibitor activity	ISO Inferred from Sequence Orthology more info
enables GTPase activating protein binding	ISO Inferred from Sequence Orthology more info
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info
enables GTPase activator activity	ISO Inferred from Sequence Orthology more info
enables GTPase activator activity	TAS Traceable Author Statement more info	PubMed
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule binding	ISO Inferred from Sequence Orthology more info
enables microtubule binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor complex adaptor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell division	ISO Inferred from Sequence Orthology more info
involved_in cell division	ISS Inferred from Sequence or Structural Similarity more info
involved_in chromosome segregation	ISO Inferred from Sequence Orthology more info
involved_in chromosome segregation	ISS Inferred from Sequence or Structural Similarity more info
involved_in learning	ISO Inferred from Sequence Orthology more info
involved_in learning	ISS Inferred from Sequence or Structural Similarity more info
involved_in long-term memory	ISO Inferred from Sequence Orthology more info
involved_in long-term memory	ISS Inferred from Sequence or Structural Similarity more info
involved_in long-term synaptic potentiation	ISO Inferred from Sequence Orthology more info
involved_in long-term synaptic potentiation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in modulation of chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of ERK1 and ERK2 cascade	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of G protein-coupled receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of signal transduction	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of synaptic plasticity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of synaptic plasticity	ISS Inferred from Sequence or Structural Similarity more info
involved_in nucleocytoplasmic transport	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleocytoplasmic transport	ISO Inferred from Sequence Orthology more info
involved_in platelet-derived growth factor receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of neurogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of G protein-coupled receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in spindle organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in spindle organization	ISO Inferred from Sequence Orthology more info
involved_in spindle organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in visual learning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in zygote asymmetric cell division	ISO Inferred from Sequence Orthology more info
involved_in zygote asymmetric cell division	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in PML body	IEA Inferred from Electronic Annotation more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in dendrite	ISS Inferred from Sequence or Structural Similarity more info
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
located_in dendritic spine	ISS Inferred from Sequence or Structural Similarity more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in nuclear body	ISS Inferred from Sequence or Structural Similarity more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic density	ISO Inferred from Sequence Orthology more info
located_in postsynaptic density	ISS Inferred from Sequence or Structural Similarity more info
located_in spindle	ISO Inferred from Sequence Orthology more info
located_in spindle	ISS Inferred from Sequence or Structural Similarity more info
located_in spindle pole	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: regulator of G-protein signaling 14

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053764.2 → NP_446216.1 regulator of G-protein signaling 14

See identical proteins and their annotated locations for NP_446216.1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000017

UniProtKB/Swiss-Prot

O08773

UniProtKB/TrEMBL

A6KAT5

Related

ENSRNOP00000021596.1, ENSRNOT00000021596.3

Conserved Domains (4) summary

cd01817
Location:301 → 374

RGS12_RBD; Ubiquitin domain of RGS12 and RGS14

cd08743
Location:58 → 186

RGS_RGS14; Regulator of G protein signaling (RGS) domain found in the RGS14 protein

smart00390
Location:497 → 519

GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

cl00155
Location:382 → 443

UBQ; Ubiquitin-like proteins

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086035.1 Reference GRCr8

Range

9254112..9268233 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063276019.1 → XP_063132089.1 regulator of G-protein signaling 14 isoform X1

UniProtKB/Swiss-Prot

O08773

UniProtKB/TrEMBL

A6KAT5
XM_063276020.1 → XP_063132090.1 regulator of G-protein signaling 14 isoform X2
XM_039095287.1 → XP_038951215.1 regulator of G-protein signaling 14 isoform X3

Conserved Domains (4) summary

smart00390
Location:344 → 366

GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

cd17137
Location:147 → 217

RBD1_RGS14; Ras-binding domain (RBD) 1 of regulator of G protein signaling 14 (RGS14)

cd17139
Location:218 → 289

RBD2_RGS14; Ras-binding domain (RBD) 2 of regulator of G protein signaling 14 (RGS14)

cl02565
Location:1 → 33

RGS; Regulator of G protein signaling (RGS) domain superfamily