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    Hecw2 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 [ Mus musculus (house mouse) ]

    Gene ID: 329152, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Hecw2provided by MGI
    Official Full Name
    HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2provided by MGI
    Primary source
    MGI:MGI:2685817
    See related
    Ensembl:ENSMUSG00000042807 AllianceGenome:MGI:2685817
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Gm971; Nedl2; mKIAA1301; A730039N16Rik; D030049F17Rik
    Summary
    Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in several processes, including cellular protein metabolic process; negative regulation of sodium ion transmembrane transporter activity; and regulation of dendrite morphogenesis. Predicted to be located in mitotic spindle. Predicted to be active in cytoplasm. Orthologous to human HECW2 (HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in cortex adult (RPKM 2.4), lung adult (RPKM 2.2) and 19 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    1 C1.1; 1 26.99 cM
    Exon count:
    32
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (53846031..54234193, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (53806872..54195034, complement)

    Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 53553 Neighboring gene dynein, axonemal, heavy chain 7A Neighboring gene STARR-seq mESC enhancer starr_00916 Neighboring gene STARR-seq mESC enhancer starr_00917 Neighboring gene STARR-seq mESC enhancer starr_00918 Neighboring gene STARR-seq mESC enhancer starr_00919 Neighboring gene STARR-seq mESC enhancer starr_00920 Neighboring gene STARR-positive B cell enhancer ABC_E7724 Neighboring gene STARR-seq mESC enhancer starr_00921 Neighboring gene STARR-seq mESC enhancer starr_00924 Neighboring gene predicted gene, 32311 Neighboring gene STARR-positive B cell enhancer ABC_E627 Neighboring gene STARR-positive B cell enhancer ABC_E1544 Neighboring gene STARR-positive B cell enhancer ABC_E3276 Neighboring gene STARR-seq mESC enhancer starr_00925 Neighboring gene serine/threonine kinase 17b (apoptosis-inducing) Neighboring gene STARR-positive B cell enhancer ABC_E7726 Neighboring gene STARR-positive B cell enhancer ABC_E5754 Neighboring gene STARR-seq mESC enhancer starr_00926 Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:53951535-53951718 Neighboring gene STARR-positive B cell enhancer ABC_E9464 Neighboring gene microRNA 7681 Neighboring gene predicted gene, 24251 Neighboring gene STARR-seq mESC enhancer starr_00927 Neighboring gene STARR-seq mESC enhancer starr_00928 Neighboring gene STARR-seq mESC enhancer starr_00929 Neighboring gene STARR-seq mESC enhancer starr_00930 Neighboring gene STARR-seq mESC enhancer starr_00931 Neighboring gene STARR-seq mESC enhancer starr_00932 Neighboring gene predicted gene, 51622 Neighboring gene predicted gene, 25829 Neighboring gene coiled-coil domain containing 150 Neighboring gene STARR-seq mESC enhancer starr_00933

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Engagement of circular RNA HECW2 in the nonautophagic role of ATG5 implicated in the endothelial-mesenchymal transition. Yang L, et al. Autophagy, 2018. PMID 29260931, Free PMC Article
    2. The kinase Grk2 regulates Nedd4/Nedd4-2-dependent control of epithelial Na+ channels. Dinudom A, et al. Proc Natl Acad Sci U S A, 2004 Aug 10. PMID 15284439, Free PMC Article
    3. NEDL1, a novel ubiquitin-protein isopeptide ligase for dishevelled-1, targets mutant superoxide dismutase-1. Miyazaki K, et al. J Biol Chem, 2004 Mar 19. PMID 14684739
    4. A novel HECT-type E3 ubiquitin ligase, NEDL2, stabilizes p73 and enhances its transcriptional activity. Miyazaki K, et al. Biochem Biophys Res Commun, 2003 Aug 15. PMID 12890487
    5. NEDL2 is an essential regulator of enteric neural development and GDNF/Ret signaling. Wei R, et al. Cell Signal, 2015 Mar. PMID 25555806

    See all (23) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. that circHECW2 regulated the EndoMT by directly binding to MIR30D, a significantly downregulated miRNA from miRNA profiling, which subsequently caused an increased expression of ATG5
      Title: Engagement of circular RNA HECW2 in the nonautophagic role of ATG5 implicated in the endothelial-mesenchymal transition.
    2. Data show that NEDL2 regulates GDNF/Ret/Akt pathway depends on its Nedd8 ligase activity rather than ubiquitin ligase activity.
      Title: NEDL2 regulates enteric nervous system and kidney development in its Nedd8 ligase activity-dependent manner.
    3. NEDL2 positively regulates enteric neural precursor proliferation through the GDNF/Akt signaling pathway.
      Title: NEDL2 is an essential regulator of enteric neural development and GDNF/Ret signaling.
    4. Grk2 phosphorylates the C terminus of the Na-channel beta subunit and renders the channels insensitive to inhibition by Nedd4-2
      Title: The kinase Grk2 regulates Nedd4/Nedd4-2-dependent control of epithelial Na+ channels.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Gene trapped (1) 
    • Targeted (3)  1 citation
    • Endonuclease-mediated (2) 

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin-protein transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in negative regulation of sodium ion transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein ubiquitination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of dendrite morphogenesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of mitotic metaphase/anaphase transition ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in ubiquitin-dependent protein catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoskeleton IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitotic spindle ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    E3 ubiquitin-protein ligase HECW2
    Names
    HECT, C2 and WW domain-containing protein 2
    HECT-type E3 ubiquitin transferase HECW2
    NEDD4-like E3 ubiquitin-protein ligase 2
    NEDD4-related E3 ubiquitin ligase NEDL2
    NP_001001883.1
    NP_766243.2
    XP_006496129.1
    XP_006496130.1
    XP_006496131.1
    XP_006496132.1
    XP_011236839.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001001883.3 → NP_001001883.1  E3 ubiquitin-protein ligase HECW2 isoform 1

      See identical proteins and their annotated locations for NP_001001883.1

      Status: VALIDATED

      Source sequence(s)
      AC124175, AC136517, AC150896
      Consensus CDS
      CCDS14956.1
      UniProtKB/Swiss-Prot
      Q3KNL8, Q6I6G8, Q6ZPU4, Q8C956
      Related
      ENSMUSP00000084942.5, ENSMUST00000087659.11
      Conserved Domains (7) summary
      pfam06390
      Location:463 → 639
      NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
      cd08691
      Location:185 → 320
      C2_NEDL1-like; C2 domain present in NEDL1 (NEDD4-like ubiquitin protein ligase-1)
      cd00078
      Location:1222 → 1576
      HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
      pfam00397
      Location:815 → 844
      WW; WW domain
      pfam07263
      Location:344 → 700
      DMP1; Dentin matrix protein 1 (DMP1)
      pfam16562
      Location:45 → 164
      HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
      pfam18436
      Location:921 → 987
      HECW1_helix; Helical box domain of E3 ubiquitin-protein ligase HECW1

    2. NM_172655.3 → NP_766243.2  E3 ubiquitin-protein ligase HECW2 isoform 2

      See identical proteins and their annotated locations for NP_766243.2

      Status: VALIDATED

      Source sequence(s)
      AC150896, AK042922
      Consensus CDS
      CCDS14957.1
      UniProtKB/TrEMBL
      A3KPB7
      Related
      ENSMUSP00000095348.3, ENSMUST00000097741.3
      Conserved Domains (2) summary
      pfam16562
      Location:45 → 164
      HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
      cl14603
      Location:185 → 294
      C2; C2 domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000067.7 Reference GRCm39 C57BL/6J

      Range
      53846031..54234193 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006496067.3 → XP_006496130.1  E3 ubiquitin-protein ligase HECW2 isoform X1

      See identical proteins and their annotated locations for XP_006496130.1

      UniProtKB/Swiss-Prot
      Q3KNL8, Q6I6G8, Q6ZPU4, Q8C956
      Conserved Domains (7) summary
      pfam06390
      Location:463 → 639
      NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
      cd08691
      Location:185 → 320
      C2_NEDL1-like; C2 domain present in NEDL1 (NEDD4-like ubiquitin protein ligase-1)
      cd00078
      Location:1222 → 1576
      HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
      pfam00397
      Location:815 → 844
      WW; WW domain
      pfam07263
      Location:344 → 700
      DMP1; Dentin matrix protein 1 (DMP1)
      pfam16562
      Location:45 → 164
      HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
      pfam18436
      Location:921 → 987
      HECW1_helix; Helical box domain of E3 ubiquitin-protein ligase HECW1

    2. XM_006496068.5 → XP_006496131.1  E3 ubiquitin-protein ligase HECW2 isoform X1

      See identical proteins and their annotated locations for XP_006496131.1

      UniProtKB/Swiss-Prot
      Q3KNL8, Q6I6G8, Q6ZPU4, Q8C956
      Conserved Domains (7) summary
      pfam06390
      Location:463 → 639
      NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
      cd08691
      Location:185 → 320
      C2_NEDL1-like; C2 domain present in NEDL1 (NEDD4-like ubiquitin protein ligase-1)
      cd00078
      Location:1222 → 1576
      HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
      pfam00397
      Location:815 → 844
      WW; WW domain
      pfam07263
      Location:344 → 700
      DMP1; Dentin matrix protein 1 (DMP1)
      pfam16562
      Location:45 → 164
      HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
      pfam18436
      Location:921 → 987
      HECW1_helix; Helical box domain of E3 ubiquitin-protein ligase HECW1

    3. XM_006496066.5 → XP_006496129.1  E3 ubiquitin-protein ligase HECW2 isoform X1

      See identical proteins and their annotated locations for XP_006496129.1

      UniProtKB/Swiss-Prot
      Q3KNL8, Q6I6G8, Q6ZPU4, Q8C956
      Related
      ENSMUSP00000113283.2, ENSMUST00000120904.8
      Conserved Domains (7) summary
      pfam06390
      Location:463 → 639
      NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
      cd08691
      Location:185 → 320
      C2_NEDL1-like; C2 domain present in NEDL1 (NEDD4-like ubiquitin protein ligase-1)
      cd00078
      Location:1222 → 1576
      HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
      pfam00397
      Location:815 → 844
      WW; WW domain
      pfam07263
      Location:344 → 700
      DMP1; Dentin matrix protein 1 (DMP1)
      pfam16562
      Location:45 → 164
      HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
      pfam18436
      Location:921 → 987
      HECW1_helix; Helical box domain of E3 ubiquitin-protein ligase HECW1

    4. XM_006496069.5 → XP_006496132.1  E3 ubiquitin-protein ligase HECW2 isoform X2

      See identical proteins and their annotated locations for XP_006496132.1

      Conserved Domains (7) summary
      pfam06390
      Location:332 → 508
      NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
      cd08691
      Location:54 → 189
      C2_NEDL1-like; C2 domain present in NEDL1 (NEDD4-like ubiquitin protein ligase-1)
      cd00078
      Location:1091 → 1445
      HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
      pfam00397
      Location:684 → 713
      WW; WW domain
      pfam07263
      Location:213 → 569
      DMP1; Dentin matrix protein 1 (DMP1)
      pfam16562
      Location:1 → 33
      HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
      pfam18436
      Location:790 → 856
      HECW1_helix; Helical box domain of E3 ubiquitin-protein ligase HECW1

    5. XM_011238537.4 → XP_011236839.1  E3 ubiquitin-protein ligase HECW2 isoform X2

      See identical proteins and their annotated locations for XP_011236839.1

      Conserved Domains (7) summary
      pfam06390
      Location:332 → 508
      NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
      cd08691
      Location:54 → 189
      C2_NEDL1-like; C2 domain present in NEDL1 (NEDD4-like ubiquitin protein ligase-1)
      cd00078
      Location:1091 → 1445
      HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
      pfam00397
      Location:684 → 713
      WW; WW domain
      pfam07263
      Location:213 → 569
      DMP1; Dentin matrix protein 1 (DMP1)
      pfam16562
      Location:1 → 33
      HECW_N; N-terminal domain of E3 ubiquitin-protein ligase HECW1 and 2
      pfam18436
      Location:790 → 856
      HECW1_helix; Helical box domain of E3 ubiquitin-protein ligase HECW1
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q6I6G8.1 GenPept UniProtKB/Swiss-Prot:Q6I6G8

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