Atp1a4 ATPase, Na+/K+ transporting, alpha 4 polypeptide [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Atp1a4provided by MGI
Official Full Name: ATPase, Na+/K+ transporting, alpha 4 polypeptideprovided by MGI
Primary source: MGI:MGI:1351335
See related: Ensembl:ENSMUSG00000007107 AllianceGenome:MGI:1351335
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables P-type sodium:potassium-exchanging transporter activity. Acts upstream of or within several processes, including flagellated sperm motility; sodium ion transport; and spermatogenesis. Predicted to be located in rod photoreceptor outer segment and sperm midpiece. Predicted to be part of sodium:potassium-exchanging ATPase complex. Is expressed in choroid plexus; epididymis; and testis. Orthologous to human ATP1A4 (ATPase Na+/K+ transporting subunit alpha 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward testis adult (RPKM 138.2) See more
Orthologs: human all
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Genomic context

Location:: 1 H3; 1 79.54 cM

Exon count:: 24

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (172051075..172085991, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (172223508..172258424, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Na,K-ATPase Atp1a4 isoform is important for maintaining sperm flagellar shape.
Title: Na,K-ATPase Atp1a4 isoform is important for maintaining sperm flagellar shape.
Ouabain interactions with the alpha4 isoform of the sodium pump trigger non-classical steroid hormone signaling and integrin expression in spermatocytes.
Title: Ouabain interactions with the α4 isoform of the sodium pump trigger non-classical steroid hormone signaling and integrin expression in spermatogenic cells.
data suggests that both the promoter and the intragenic CGI could combine to provide multiple modes of regulation for optimizing the Atp1a4 expression level in a cell type-specific manner.
Title: Methylation-dependent and independent regulatory regions in the Na,K-ATPase alpha4 (Atp1a4) gene may impact its testis-specific expression.
These findings highlight the exquisite spatial and temporal control of expression exerted by the Atp1a4 promoter on Na,K-ATPase alpha4, which is particularly well suited to fulfill the special functions of spermatozoa.
Title: Green fluorescence protein driven by the Na,K-ATPase α4 isoform promoter is expressed only in male germ cells of mouse testis.
Na,K-ATPase alpha4 isoform is essential for sperm fertility
Title: Na,K-ATPase alpha4 isoform is essential for sperm fertility.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Atp1a4 (e-PCR), detects polymorphism
- UniSTS:480321

Atp1a4 (e-PCR), detects polymorphism
- UniSTS:546667

D1Sta3 (e-PCR), detects polymorphism
- UniSTS:142273

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATPase-coupled monoatomic cation transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables P-type potassium transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables P-type sodium:potassium-exchanging transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables P-type sodium:potassium-exchanging transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables P-type sodium:potassium-exchanging transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables P-type sodium:potassium-exchanging transporter activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within establishment of localization in cell	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within flagellated sperm motility	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in flagellated sperm motility	ISO Inferred from Sequence Orthology more info
involved_in intracellular potassium ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular sodium ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within monoatomic ion transport	IEA Inferred from Electronic Annotation more info
involved_in potassium ion import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in potassium ion transmembrane transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within potassium ion transport	IEA Inferred from Electronic Annotation more info
involved_in potassium ion transport	IEA Inferred from Electronic Annotation more info
involved_in proton transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cellular pH	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sodium ion export across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in sodium ion transmembrane transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within sodium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cell projection	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in photoreceptor cell cilium	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in rod photoreceptor outer segment	ISO Inferred from Sequence Orthology more info
part_of sodium:potassium-exchanging ATPase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of sodium:potassium-exchanging ATPase complex	ISO Inferred from Sequence Orthology more info
located_in sperm midpiece	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: sodium/potassium-transporting ATPase subunit alpha-4

Names: Na(+)/K(+) ATPase alpha-4 subunit; Na+/K+ ATPase alpha 4 subunit; sodium pump subunit alpha-4; sodium/potassium-transporting ATPase alpha-4 chain

NP_038762.1

EC 7.2.2.13

XP_006496937.1

EC 7.2.2.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_013734.1 → NP_038762.1 sodium/potassium-transporting ATPase subunit alpha-4

See identical proteins and their annotated locations for NP_038762.1

Status: VALIDATED

Source sequence(s)

AC074310, AC074311, AC087061

Consensus CDS

CCDS48446.1

UniProtKB/Swiss-Prot

E9QKL5, Q9R173, Q9WV27, Q9WV28

Related

ENSMUSP00000106874.2, ENSMUST00000111243.2

Conserved Domains (6) summary

smart00831
Location:50 → 123

Cation_ATPase_N; Cation transporter/ATPase, N-terminus

COG4087
Location:696 → 761

COG4087; Soluble P-type ATPase [General function prediction only]

TIGR01106
Location:36 → 1032

ATPase-IIC_X-K; sodium or proton efflux -- potassium uptake antiporter, P-type ATPase, alpha subunit

pfam00122
Location:144 → 375

E1-E2_ATPase; E1-E2 ATPase

pfam00689
Location:808 → 1017

Cation_ATPase_C; Cation transporting ATPase, C-terminus

pfam13246
Location:438 → 530

Cation_ATPase; Cation transport ATPase (P-type)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

172051075..172085991 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006496874.3 → XP_006496937.1 sodium/potassium-transporting ATPase subunit alpha-4 isoform X1

Conserved Domains (1) summary

TIGR01106
Location:39 → 728

ATPase-IIC_X-K; sodium or proton efflux -- potassium uptake antiporter, P-type ATPase, alpha subunit