DCLK1 doublecortin like kinase 1 [Homo sapiens (human)] - Gene

Summary

Official Symbol: DCLK1provided by HGNC
Official Full Name: doublecortin like kinase 1provided by HGNC
Primary source: HGNC:HGNC:2700
See related: Ensembl:ENSG00000133083 MIM:604742; AllianceGenome:HGNC:2700
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: CL1; DCLK; CLICK1; DCDC3A; DCAMKL1
Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010]
Expression: Biased expression in brain (RPKM 28.5), fat (RPKM 4.0) and 7 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 13q13.3

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	13	NC_000013.11 (35768652..36131939, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	13	NC_060937.1 (34986929..35351445, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	13	NC_000013.10 (36342789..36706076, complement)

Chromosome 13 - NC_000013.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DCLK1 and its oncogenic functions: A promising therapeutic target for cancers.
Title: DCLK1 and its oncogenic functions: A promising therapeutic target for cancers.
Role of DCLK1/Hippo pathway in type II alveolar epithelial cells differentiation in acute respiratory distress syndrome.
Title: Role of DCLK1/Hippo pathway in type II alveolar epithelial cells differentiation in acute respiratory distress syndrome.
Exosomes from metastatic colon cancer cells drive the proliferation and migration of primary colon cancer through increased expression of cancer stem cell markers CD133 and DCLK1.
Title: Exosomes from metastatic colon cancer cells drive the proliferation and migration of primary colon cancer through increased expression of cancer stem cell markers CD133 and DCLK1.
[Doublecortin-like kinase 1 activates Hippo pathway to promote migration, invasion and proliferation of pancreatic cancer cells].
Title: [Doublecortin-like kinase 1 activates Hippo pathway to promote migration, invasion and proliferation of pancreatic cancer cells].
Targeting DCLK1 attenuates tumor stemness and evokes antitumor immunity in triple-negative breast cancer by inhibiting IL-6/STAT3 signaling.
Title: Targeting DCLK1 attenuates tumor stemness and evokes antitumor immunity in triple-negative breast cancer by inhibiting IL-6/STAT3 signaling.
Doublecortin-like kinase 1 is a therapeutic target in squamous cell carcinoma.
Title: Doublecortin-like kinase 1 is a therapeutic target in squamous cell carcinoma.
KDM3A promotes oral squamous cell carcinoma cell proliferation and invasion via H3K9me2 demethylation-activated DCLK1.
Title: KDM3A promotes oral squamous cell carcinoma cell proliferation and invasion via H3K9me2 demethylation-activated DCLK1.
Targeting Doublecortin-Like Kinase 1 (DCLK1)-Regulated SARS-CoV-2 Pathogenesis in COVID-19.
Title: Targeting Doublecortin-Like Kinase 1 (DCLK1)-Regulated SARS-CoV-2 Pathogenesis in COVID-19.
DCLK1 promotes colorectal cancer stemness and aggressiveness via the XRCC5/COX2 axis.
Title: DCLK1 promotes colorectal cancer stemness and aggressiveness via the XRCC5/COX2 axis.
Targeting DCLK1 overcomes 5-fluorouracil resistance in colorectal cancer through inhibiting CCAR1/beta-catenin pathway-mediated cancer stemness.
Title: Targeting DCLK1 overcomes 5-fluorouracil resistance in colorectal cancer through inhibiting CCAR1/β-catenin pathway-mediated cancer stemness.

Submit: New GeneRIF Correction See all GeneRIFs (96)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
A genome-wide association study of optic disc parameters. EBI GWAS Catalog EBI GWAS CatalogPubMed
Genetic variants associated with breast size also influence breast cancer risk. EBI GWAS Catalog EBI GWAS CatalogPubMed
Genome-wide association study of electrocardiographic and heart rate variability traits: the Framingham Heart Study. EBI GWAS Catalog EBI GWAS CatalogPubMed
Genome-wide association study of the age of onset of childhood asthma. EBI GWAS Catalog EBI GWAS CatalogPubMed
Genome-wide scan revealed that polymorphisms in the PNPLA3, SAMM50, and PARVB genes are associated with development and progression of nonalcoholic fatty liver disease in Japan. EBI GWAS Catalog EBI GWAS CatalogPubMed
Identification, replication, and fine-mapping of Loci associated with adult height in individuals of african ancestry. EBI GWAS Catalog EBI GWAS CatalogPubMed
Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

DCAMKL1_4674 (e-PCR)
- UniSTS:462855

WI-21179 (e-PCR)
- UniSTS:24844

RH78794 (e-PCR)
- UniSTS:59670

D13S849E (e-PCR)
- UniSTS:21030

G17692 (e-PCR)
- UniSTS:69170

WI-14261 (e-PCR)
- UniSTS:1347

D13S1174 (e-PCR)
- UniSTS:68696

D13S1164 (e-PCR)
- UniSTS:19804

RH46169 (e-PCR)
- UniSTS:55141

AFMB316WB5 (e-PCR)
- UniSTS:47462

A008Z44 (e-PCR)
- UniSTS:32617

RH44822 (e-PCR)
- UniSTS:44656

SHGC-80915 (e-PCR)
- UniSTS:102554

G65669 (e-PCR)
- UniSTS:225460

SHGC-105091 (e-PCR)
- UniSTS:169142

RH119982 (e-PCR)
- UniSTS:133306

RH11210 (e-PCR)
- UniSTS:81851

D13S1159 (e-PCR)
- UniSTS:60104

RH121485 (e-PCR)
- UniSTS:134613

SHGC-103675 (e-PCR)
- UniSTS:168922

SHGC-56401 (e-PCR)
- UniSTS:28385

D13S240 (e-PCR)
- UniSTS:27880

RH123792 (e-PCR)
- UniSTS:136169

RH44747 (e-PCR)
- UniSTS:39658

STS-Z40654 (e-PCR)
- UniSTS:56826

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	TAS Traceable Author Statement more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in axon extension	IEA Inferred from Electronic Annotation more info
involved_in central nervous system development	TAS Traceable Author Statement more info	PubMed
involved_in central nervous system projection neuron axonogenesis	IEA Inferred from Electronic Annotation more info
involved_in dendrite morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in endosomal transport	NAS Non-traceable Author Statement more info	PubMed
involved_in forebrain development	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of protein localization to nucleus	IEA Inferred from Electronic Annotation more info
involved_in nervous system development	TAS Traceable Author Statement more info	PubMed
involved_in neuron migration	IEA Inferred from Electronic Annotation more info
involved_in neuron projection morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein localization to nucleus	IEA Inferred from Electronic Annotation more info
involved_in protein phosphorylation	TAS Traceable Author Statement more info	PubMed
involved_in response to virus	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in plasma membrane	TAS Traceable Author Statement more info	PubMed
located_in postsynaptic density	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: serine/threonine-protein kinase DCLK1

Names: doublecortin domain-containing protein 3A; doublecortin-like and CAM kinase-like 1

NP_001182344.1

EC 2.7.11.1

NP_001182345.1

EC 2.7.11.1

NP_001182359.1

EC 2.7.11.1

NP_001317000.1

EC 2.7.11.1

NP_001317001.1

EC 2.7.11.1

NP_004725.1

EC 2.7.11.1

XP_016876336.1

EC 2.7.11.1

XP_016876337.1

EC 2.7.11.1

XP_047286723.1

EC 2.7.11.1

XP_054231145.1

EC 2.7.11.1

XP_054231146.1

EC 2.7.11.1

XP_054231147.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001195415.2 → NP_001182344.1 serine/threonine-protein kinase DCLK1 isoform 2

See identical proteins and their annotated locations for NP_001182344.1

Status: REVIEWED

Description

Transcript Variant: This variant (2, also known as DCLK-short-B, DCK-beta1 or KIAA0369-BS) is produced from the 3' promoter. It lacks several 5' exons but has an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a shorter and different N-terminus, and lacks doublecortin domains, as compared to isoform 1.

Source sequence(s)

AK295777, AL139083, AL157760

Consensus CDS

CCDS73561.1

UniProtKB/TrEMBL

B7Z5K4

Related

ENSP00000484452.1, ENST00000615680.5

Conserved Domains (1) summary

cd14183
Location:76 → 343

STKc_DCKL1; Catalytic domain of the Serine/Threonine Kinase, Doublecortin-like kinase 1 (also called Doublecortin-like and CAM kinase-like 1)
NM_001195416.2 → NP_001182345.1 serine/threonine-protein kinase DCLK1 isoform 3

See identical proteins and their annotated locations for NP_001182345.1

Status: REVIEWED

Description

Transcript Variant: This variant [3, also known as DCLK-short-A, DCK-beta2, KIAA0369-BL or cpg16 (candidate plasticity gene 16)] is produced from the 3' promoter. It lacks several 5' exons but has an alternate 5' exon, and lacks a coding exon in the 3' region that results in a frame-shift, as compared to variant 1. The resulting isoform (3) has a shorter and different N-terminus and a longer and distinct C-terminus, and lacks doublecortin domains, as compared to isoform 1.

Source sequence(s)

AK299059, AK311195, AL139083, AL157760, BC152456

Consensus CDS

CCDS55895.1

UniProtKB/TrEMBL

B7Z5K4

Related

ENSP00000369223.1, ENST00000379893.5

Conserved Domains (1) summary

cd14183
Location:76 → 343

STKc_DCKL1; Catalytic domain of the Serine/Threonine Kinase, Doublecortin-like kinase 1 (also called Doublecortin-like and CAM kinase-like 1)
NM_001195430.2 → NP_001182359.1 serine/threonine-protein kinase DCLK1 isoform 4

See identical proteins and their annotated locations for NP_001182359.1

Status: REVIEWED

Description

Transcript Variant: This variant (4) is produced from the 3' promoter. It shares only one exon with variant 1, and encodes a 56 aa long isoform [4, also known as ania-4, or CARP (CaMK-related peptide)], which mainly consists of the serine/proline-rich domain.

Source sequence(s)

AL157760, BM682165

UniProtKB/TrEMBL

A0A9L9PXT2

Related

ENSP00000516676.1, ENST00000460982.1
NM_001330071.2 → NP_001317000.1 serine/threonine-protein kinase DCLK1 isoform 5

Status: REVIEWED

Description

Transcript Variant: This variant (5) lacks an alternate exon in the 3' coding region, resulting in a frameshift compared to variant 1. Both variants 5 and 6 encode the same isoform (5), which is longer and has a distinct C-terminus compared to isoform 1.

Source sequence(s)

AK295777, AL139083, AL157760, AL160392, BC152456, BM679448, DB453639, DC350316, HY138649

Consensus CDS

CCDS81762.1

UniProtKB/Swiss-Prot

B7Z3E9, O15075, Q5VZY8, Q5VZZ0, Q5VZZ1

UniProtKB/TrEMBL

B7Z2T7

Related

ENSP00000353846.3, ENST00000360631.8

Conserved Domains (3) summary

cd14183
Location:383 → 650

STKc_DCKL1; Catalytic domain of the Serine/Threonine Kinase, Doublecortin-like kinase 1 (also called Doublecortin-like and CAM kinase-like 1)

cd17069
Location:182 → 265

DCX2; Dublecortin-like domain 2

cd17140
Location:55 → 143

DCX1_DCLK1; Dublecortin-like domain 1 found in doublecortin-like kinase 1 (DCLK1)
NM_001330072.2 → NP_001317001.1 serine/threonine-protein kinase DCLK1 isoform 5

Status: REVIEWED

Description

Transcript Variant: This variant (6) differs in the 5' UTR and lacks an alternate exon in the 3' coding region, resulting in a frameshift compared to variant 1. Both variants 5 and 6 encode the same isoform (5), which is longer and has a distinct C-terminus compared to isoform 1.

Source sequence(s)

AK295777, AL139083, AL157760, BC152456, BM679448, DA154341, DC350316

Consensus CDS

CCDS81762.1

UniProtKB/Swiss-Prot

B7Z3E9, O15075, Q5VZY8, Q5VZZ0, Q5VZZ1

UniProtKB/TrEMBL

B7Z2T7

Conserved Domains (3) summary

cd14183
Location:383 → 650

STKc_DCKL1; Catalytic domain of the Serine/Threonine Kinase, Doublecortin-like kinase 1 (also called Doublecortin-like and CAM kinase-like 1)

cd17069
Location:182 → 265

DCX2; Dublecortin-like domain 2

cd17140
Location:55 → 143

DCX1_DCLK1; Dublecortin-like domain 1 found in doublecortin-like kinase 1 (DCLK1)
NM_004734.5 → NP_004725.1 serine/threonine-protein kinase DCLK1 isoform 1

See identical proteins and their annotated locations for NP_004725.1

Status: REVIEWED

Description

Transcript Variant: This variant (1, also known as DCLK-long-B, DCK-alpha1 or KIAA0369AS) is produced from the 5' promoter. It encodes the isoform 1, which includes two doublecortin domains, a serine/proline-rich domain and a protein kinase domain.

Source sequence(s)

AK295777, AL139083, AL157760, AL160392, BC152456

Consensus CDS

CCDS9354.1

UniProtKB/TrEMBL

B7Z2T7

Related

ENSP00000255448.4, ENST00000255448.8

Conserved Domains (3) summary

cd14183
Location:383 → 650

STKc_DCKL1; Catalytic domain of the Serine/Threonine Kinase, Doublecortin-like kinase 1 (also called Doublecortin-like and CAM kinase-like 1)

cd17069
Location:182 → 265

DCX2; Dublecortin-like domain 2

cd17140
Location:55 → 143

DCX1_DCLK1; Dublecortin-like domain 1 found in doublecortin-like kinase 1 (DCLK1)

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000013.11 Reference GRCh38.p14 Primary Assembly

Range

35768652..36131939 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017020847.2 → XP_016876336.1 serine/threonine-protein kinase DCLK1 isoform X1

UniProtKB/TrEMBL

B7Z2T7

Conserved Domains (3) summary

cd14183
Location:383 → 650

STKc_DCKL1; Catalytic domain of the Serine/Threonine Kinase, Doublecortin-like kinase 1 (also called Doublecortin-like and CAM kinase-like 1)

cd17069
Location:182 → 265

DCX2; Dublecortin-like domain 2

cd17140
Location:55 → 143

DCX1_DCLK1; Dublecortin-like domain 1 found in doublecortin-like kinase 1 (DCLK1)
XM_047430767.1 → XP_047286723.1 serine/threonine-protein kinase DCLK1 isoform X2

UniProtKB/TrEMBL

Q5VZY9
XM_017020848.2 → XP_016876337.1 serine/threonine-protein kinase DCLK1 isoform X2

UniProtKB/TrEMBL

Q5VZY9

Related

ENSP00000369222.4, ENST00000379892.4

Conserved Domains (2) summary

cd17069
Location:182 → 265

DCX2; Dublecortin-like domain 2

cd17140
Location:55 → 143

DCX1_DCLK1; Dublecortin-like domain 1 found in doublecortin-like kinase 1 (DCLK1)

Alternate T2T-CHM13v2.0

Genomic

NC_060937.1 Alternate T2T-CHM13v2.0

Range

34986929..35351445 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054375170.1 → XP_054231145.1 serine/threonine-protein kinase DCLK1 isoform X1
XM_054375172.1 → XP_054231147.1 serine/threonine-protein kinase DCLK1 isoform X2

UniProtKB/TrEMBL

Q5VZY9
XM_054375171.1 → XP_054231146.1 serine/threonine-protein kinase DCLK1 isoform X2

UniProtKB/TrEMBL

Q5VZY9