Nek2 NIMA (never in mitosis gene a)-related expressed kinase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nek2provided by MGI
Official Full Name: NIMA (never in mitosis gene a)-related expressed kinase 2provided by MGI
Primary source: MGI:MGI:109359
See related: Ensembl:ENSMUSG00000026622 AllianceGenome:MGI:109359
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables protein kinase activity. Acts upstream of or within several processes, including blastocyst development; mitotic nuclear division; and negative regulation of DNA binding activity. Located in condensed nuclear chromosome; midbody; and spindle pole. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; immune system; and respiratory system. Human ortholog(s) of this gene implicated in retinitis pigmentosa 67. Orthologous to human NEK2 (NIMA related kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in testis adult (RPKM 25.4), CNS E11.5 (RPKM 10.9) and 15 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 H6; 1 96.94 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (191553622..191565161)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (191821449..191833049)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Involvement of NEK2 and NEK9 in LPS - induced endothelial barrier dysfunction.
Title: Involvement of NEK2 and NEK9 in LPS - induced endothelial barrier dysfunction.
NEK2 overexpression aggravates IL-22-induced keratinocyte proliferation and cytokine level increases and IMQ-induced psoriasis-like dermatitis.
Title: NEK2 overexpression aggravates IL-22-induced keratinocyte proliferation and cytokine level increases and IMQ-induced psoriasis-like dermatitis.
[NEK2 gene expression in mouse cryptorchidism model and its mechanism involved in apoptosis].
Title: [NEK2 gene expression in mouse cryptorchidism model and its mechanism involved in apoptosis].
the present study evaluates the effects of miR-138 on chondrocyte proliferation, differentiation, and apoptosis through the WNT/beta-catenin signaling pathway in mice with OA by binding to NIMA-related kinase 2 (NEK2)
Title: microRNA-138 induces cell survival and reduces WNT/β-catenin signaling of osteoarthritis chondrocytes through NEK2.
Cep85 is a bona fide Nek2A-binding partner that surrounds the proximal ends of centrioles where it cooperates with PP1gamma (also known as PPP1CC) to antagonize Nek2A
Title: Characterization of Cep85 - a new antagonist of Nek2A that is involved in the regulation of centrosome disjunction.
The results suggest that overexpression of NEK2 has the potential to serve as a biomarker for PCa prognosis.
Title: Overexpression of NIMA-related kinase 2 is associated with progression and poor prognosis of prostate cancer.
Nek2 transgenic mice develop spontaneous germinal centers and exhibit an enhanced T cell dependent immune response our data demonstrates a novel role for Nek2 in regulating B cell development and the immune response
Title: Nek2 is a novel regulator of B cell development and immunological response.
these data indicate that Nek2 has a pivotal role in breast cancer growth at primary and secondary sites, and thus may be an attractive and novel therapeutic target for this disease.
Title: Role of Nek2 on centrosome duplication and aneuploidy in breast cancer cells.
CIP2A strongly interacts with NEK2 during G2/M phase, thereby enhancing NEK2 kinase activity to facilitate centrosome separation in a PP1- and PP2A-independent manner.
Title: Cancerous inhibitor of protein phosphatase 2A (CIP2A) protein is involved in centrosome separation through the regulation of NIMA (never in mitosis gene A)-related kinase 2 (NEK2) protein activity.
Nek2 and its substrate, centrobin/Nip2, are required for proper meiotic spindle formation of the mouse oocytes
Title: Nek2 and its substrate, centrobin/Nip2, are required for proper meiotic spindle formation of the mouse oocytes.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2)
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Nek2 (e-PCR), detects polymorphism
- UniSTS:472839

RH124524 (e-PCR)
- UniSTS:161346

AF007247 (e-PCR)
- UniSTS:178554

BE688013 (e-PCR)
- UniSTS:207274

RH126090 (e-PCR)
- UniSTS:162802

UniSTS:465399 (e-PCR)
- UniSTS:465399

Nek2 (e-PCR), detects polymorphism
- UniSTS:465399

Nek2 (e-PCR), detects polymorphism
- UniSTS:465400

Nek2 (e-PCR), detects polymorphism
- UniSTS:465401

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein phosphatase binding	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within blastocyst development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
involved_in centrosome separation	ISO Inferred from Sequence Orthology more info
involved_in chromosome segregation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromosome segregation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within meiotic cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within mitotic sister chromatid segregation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mitotic spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of DNA binding	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of centriole-centriole cohesion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of telomerase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of telomere capping	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of telomere maintenance via telomerase	ISO Inferred from Sequence Orthology more info
involved_in protein autophosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of attachment of spindle microtubules to kinetochore	ISO Inferred from Sequence Orthology more info
involved_in regulation of mitotic centrosome separation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in condensed nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
colocalizes_with kinetochore	ISO Inferred from Sequence Orthology more info
located_in kinetochore	ISO Inferred from Sequence Orthology more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in midbody	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
located_in spindle pole	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: serine/threonine-protein kinase Nek2

Names: NIMA - related expressed kinase 2; NIMA-related expressed kinase 2; NIMA-related kinase 2; Nek2 kinase; never in mitosis A-related kinase 2; nimA-related protein kinase 2

NP_001407321.1

EC 2.7.11.1

NP_001407322.1

EC 2.7.11.1

NP_001407323.1

EC 2.7.11.1

NP_035022.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001420392.1 → NP_001407321.1 serine/threonine-protein kinase Nek2 isoform 2

Status: VALIDATED

Source sequence(s)

AC114603
NM_001420393.1 → NP_001407322.1 serine/threonine-protein kinase Nek2 isoform 3

Status: VALIDATED

Source sequence(s)

AC114603
NM_001420394.1 → NP_001407323.1 serine/threonine-protein kinase Nek2 isoform 4

Status: VALIDATED

Source sequence(s)

AC114603

UniProtKB/TrEMBL

Q8BP64
NM_010892.4 → NP_035022.2 serine/threonine-protein kinase Nek2 isoform 1

See identical proteins and their annotated locations for NP_035022.2

Status: VALIDATED

Source sequence(s)

AC114603

Consensus CDS

CCDS15623.1

UniProtKB/Swiss-Prot

O35942, O35959, Q3TPD7

UniProtKB/TrEMBL

Q6PFG1

Related

ENSMUSP00000027931.8, ENSMUST00000027931.8

Conserved Domains (2) summary

smart00220
Location:8 → 271

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd08217
Location:7 → 271

STKc_Nek2; Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 2