Alox5 arachidonate 5-lipoxygenase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Alox5provided by RGD
Official Full Name: arachidonate 5-lipoxygenaseprovided by RGD
Primary source: RGD:2096
See related: Ensembl:ENSRNOG00000012972 AllianceGenome:RGD:2096
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: LOX5A
Summary: Predicted to enable arachidonate 5-lipoxygenase activity and iron ion binding activity. Involved in several processes, including positive regulation of cytochrome-c oxidase activity; positive regulation of vasoconstriction; and response to hyperoxia. Located in several cellular components, including dendrite; nuclear envelope; and sarcolemma. Used to study several diseases, including brain ischemia; hypertension (multiple); pleurisy; portal hypertension; and pulmonary edema. Biomarker of pulmonary hypertension. Human ortholog(s) of this gene implicated in artery disease (multiple); asthma (multiple); pancreatic cancer; and pulmonary tuberculosis. Orthologous to human ALOX5 (arachidonate 5-lipoxygenase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Lung (RPKM 53.5), Heart (RPKM 25.7) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 4q42

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	4	NC_086022.1 (151203948..151251126, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	4	NC_051339.1 (149531329..149578696, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	4	NC_005103.4 (148398004..148446308, complement)

Chromosome 4 - NC_086022.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MALAT1 binds to miR-188-3p to regulate ALOX5 activity in the lung inflammatory response of neonatal bronchopulmonary dysplasia.
Title: MALAT1 binds to miR-188-3p to regulate ALOX5 activity in the lung inflammatory response of neonatal bronchopulmonary dysplasia.
Diminished arachidonate 5-lipoxygenase perturbs phase separation and transcriptional response of Runx2 to reverse pathological ventricular remodeling.
Title: Diminished arachidonate 5-lipoxygenase perturbs phase separation and transcriptional response of Runx2 to reverse pathological ventricular remodeling.
The time-dependent reorganization has been reported of both 5-LO and FLAP into higher-order assemblies or clusters in the process of leukotriene C4 biosynthesis on the nuclear envelope in response to cell activation via the IgE receptor.
Title: The organization of leukotriene biosynthesis on the nuclear envelope revealed by single molecule localization microscopy and computational analyses.
HDLHealthy treatment enhanced the proteasome-mediated degradation of the LTB4-producing enzyme 5-lipoxygenase (LO) in activated macrophages; however, HDLCAD did not show these anti-inflammatory effects.
Title: Novel mechanism of regulation of the 5-lipoxygenase/leukotriene B(4) pathway by high-density lipoprotein in macrophages.
Study results suggest that the association of ALOX5/ALOX5AP contributes to leukotriene-induced inflammation and predisposes the trauma and hemorrhagic shock rats to lung injury.
Title: Trauma and hemorrhagic shock activate molecular association of 5-lipoxygenase and 5-lipoxygenase-Activating protein in lung tissue.
5-LOX plays an important role in activating apoptosis and repressing neutrophils activation during acute necrotizing pancreatitis.
Title: Inhibition of 5-lipoxygenase represses neutrophils activation and activates apoptosis in pancreatic tissues during acute necrotizing pancreatitis.
Our results indicate that 5-lipoxygenase activation is mechanistically involved in chronic hepatic injury in a rat model of chronic aluminum overload exposure and that the 5-LO signaling pathway, which associated with inflammation and oxidative stress, is a potential therapeutic target for chronic non-infection liver diseases
Title: 5-lipoxygenase activation is involved in the mechanisms of chronic hepatic injury in a rat model of chronic aluminum overload exposure.
Results support 5-lipoxygenase involvement in the effect of glutoxim and molixan on intracellular Ca(2+) concentration in macrophages and indicate the inadvisability of a combined use of drugs glutoxim and molixan and antiasthmatic agent zileuton
Title: 5-Lipoxygenase inhibitor zileuton inhibits Ca(2+)-responses induced by glutoxim and molixan in macrophages.
12/15-LO-derived oxidized lipids regulate histone modifications associated with profibrotic gene expression in MCs, and 12/15-LO can mediate similar actions of TGF-beta1 and diabetes.
Title: Epigenetic Histone Modifications Involved in Profibrotic Gene Regulation by 12/15-Lipoxygenase and Its Oxidized Lipid Products in Diabetic Nephropathy.
Alox5 affects the osteogenic and adipogenic abilities of iPSCs in vivo and the effect of Cox2 inhibition in this system.
Title: Deletion of Alox5 gene decreases osteogenic differentiation but increases adipogenic differentiation of mouse induced pluripotent stem cells.

Submit: New GeneRIF Correction See all GeneRIFs (34)

General gene information

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Markers

RH133456 (e-PCR)
- UniSTS:216739

BM387192 (e-PCR)
- UniSTS:261331

RH94554 (e-PCR)
- UniSTS:119802

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables arachidonate 12(S)-lipoxygenase activity	IEA Inferred from Electronic Annotation more info
enables arachidonate 5-lipoxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables arachidonate 5-lipoxygenase activity	IEA Inferred from Electronic Annotation more info
enables arachidonate 5-lipoxygenase activity	ISO Inferred from Sequence Orthology more info
enables arachidonate 5-lipoxygenase activity	ISS Inferred from Sequence or Structural Similarity more info
enables arachidonate 8(S)-lipoxygenase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables iron ion binding	ISO Inferred from Sequence Orthology more info
enables iron ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in arachidonic acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in dendritic cell migration	IEA Inferred from Electronic Annotation more info
involved_in dendritic cell migration	ISO Inferred from Sequence Orthology more info
involved_in dendritic cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in glucose homeostasis	IEA Inferred from Electronic Annotation more info
involved_in glucose homeostasis	ISO Inferred from Sequence Orthology more info
involved_in glucose homeostasis	ISS Inferred from Sequence or Structural Similarity more info
involved_in humoral immune response	IEA Inferred from Electronic Annotation more info
involved_in humoral immune response	ISO Inferred from Sequence Orthology more info
involved_in humoral immune response	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in leukocyte chemotaxis involved in inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in leukocyte chemotaxis involved in inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in leukocyte chemotaxis involved in inflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in leukocyte migration involved in inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in leukocyte migration involved in inflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in leukotriene A4 biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in leukotriene A4 biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in leukotriene A4 biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within leukotriene biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in leukotriene biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in leukotriene biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in leukotriene biosynthetic process	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within leukotriene metabolic process	ISO Inferred from Sequence Orthology more info
involved_in leukotriene production involved in inflammatory response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within leukotriene production involved in inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in lipid oxidation	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid oxidation	IEA Inferred from Electronic Annotation more info
involved_in lipoxin biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in lipoxin biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in lipoxin biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in lipoxygenase pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of angiogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of endothelial cell proliferation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of endothelial cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of endothelial cell proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of inflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of response to endoplasmic reticulum stress	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of response to endoplasmic reticulum stress	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of response to endoplasmic reticulum stress	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of sprouting angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of sprouting angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of sprouting angiogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of vascular wound healing	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of vascular wound healing	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of vascular wound healing	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of wound healing	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of wound healing	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of bone mineralization	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of bone mineralization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of bone mineralization	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of leukocyte adhesion to arterial endothelial cell	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of leukocyte adhesion to arterial endothelial cell	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of leukocyte adhesion to arterial endothelial cell	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of vasoconstriction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cellular response to oxidative stress	IEA Inferred from Electronic Annotation more info
involved_in regulation of cellular response to oxidative stress	ISO Inferred from Sequence Orthology more info
involved_in regulation of cellular response to oxidative stress	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cytokine production involved in inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in regulation of cytokine production involved in inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in regulation of cytokine production involved in inflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of fat cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in regulation of fat cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in regulation of fat cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in regulation of inflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of inflammatory response to wounding	IEA Inferred from Electronic Annotation more info
involved_in regulation of inflammatory response to wounding	ISO Inferred from Sequence Orthology more info
involved_in regulation of inflammatory response to wounding	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of insulin secretion	IEA Inferred from Electronic Annotation more info
involved_in regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
involved_in regulation of insulin secretion	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of reactive oxygen species biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in regulation of reactive oxygen species biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of reactive oxygen species biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to hyperoxia	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nutrient	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
is_active_in nuclear envelope	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear envelope	ISO Inferred from Sequence Orthology more info
located_in nuclear envelope	TAS Traceable Author Statement more info	PubMed
located_in nuclear envelope lumen	IEA Inferred from Electronic Annotation more info
located_in nuclear envelope lumen	ISO Inferred from Sequence Orthology more info
located_in nuclear envelope lumen	ISS Inferred from Sequence or Structural Similarity more info
located_in nuclear matrix	IEA Inferred from Electronic Annotation more info
located_in nuclear matrix	ISO Inferred from Sequence Orthology more info
located_in nuclear membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear membrane	ISO Inferred from Sequence Orthology more info
located_in nuclear membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in sarcolemma	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: polyunsaturated fatty acid 5-lipoxygenase

Names: 5 - Lipoxygenase; 5-LO; 5-lipoxygenase

NP_036954.2

EC 1.13.11.34

XP_038963034.1

EC 1.13.11.34

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_012822.2 → NP_036954.2 polyunsaturated fatty acid 5-lipoxygenase

Status: VALIDATED

Source sequence(s)

JAXUCZ010000004

UniProtKB/Swiss-Prot

P12527

UniProtKB/TrEMBL

F1LMM5

Related

ENSRNOP00000017633.3, ENSRNOT00000017633.6

Conserved Domains (2) summary

cd01753
Location:2 → 115

PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...

pfam00305
Location:221 → 665

Lipoxygenase

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086022.1 Reference GRCr8

Range

151203948..151251126 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039107106.2 → XP_038963034.1 polyunsaturated fatty acid 5-lipoxygenase isoform X1

UniProtKB/TrEMBL

A6IL16

Conserved Domains (1) summary

pfam00305
Location:79 → 520

Lipoxygenase