Gfi1 growth factor independent 1 transcription repressor [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gfi1provided by MGI
Official Full Name: growth factor independent 1 transcription repressorprovided by MGI
Primary source: MGI:MGI:103170
See related: Ensembl:ENSMUSG00000029275 AllianceGenome:MGI:103170
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pal1; Gfi-1; Pal-1
Summary: Predicted to enable DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in negative regulation of NF-kappaB transcription factor activity and negative regulation of transcription by RNA polymerase II. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; inner ear development; and regulation of cell fate specification. Predicted to be located in nuclear body and nuclear matrix. Predicted to be part of transcription repressor complex. Is expressed in several structures, including gut epithelium; hemolymphoid system; liver; nervous system; and sensory organ. Used to study severe congenital neutropenia. Human ortholog(s) of this gene implicated in acute myeloid leukemia and severe congenital neutropenia 2. Orthologous to human GFI1 (growth factor independent 1 transcriptional repressor). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in thymus adult (RPKM 40.7), colon adult (RPKM 6.0) and 3 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 F; 5 52.23 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (107864521..107873671, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (107716655..107725805, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Deciphering the genetic interactions between Pou4f3, Gfi1, and Rbm24 in maintaining mouse cochlear hair cell survival.
Title: Deciphering the genetic interactions between Pou4f3, Gfi1, and Rbm24 in maintaining mouse cochlear hair cell survival.
Cellular reprogramming with ATOH1, GFI1, and POU4F3 implicate epigenetic changes and cell-cell signaling as obstacles to hair cell regeneration in mature mammals.
Title: Cellular reprogramming with ATOH1, GFI1, and POU4F3 implicate epigenetic changes and cell-cell signaling as obstacles to hair cell regeneration in mature mammals.
GFI1 regulates hair cell differentiation by acting as an off-DNA transcriptional co-activator of ATOH1, and a DNA-binding repressor.
Title: GFI1 regulates hair cell differentiation by acting as an off-DNA transcriptional co-activator of ATOH1, and a DNA-binding repressor.
Generation of mature and functional hair cells by co-expression of Gfi1, Pou4f3, and Atoh1 in the postnatal mouse cochlea.
Title: Generation of mature and functional hair cells by co-expression of Gfi1, Pou4f3, and Atoh1 in the postnatal mouse cochlea.
Gfi1 Loss Protects against Two Models of Induced Diabetes.
Title: Gfi1 Loss Protects against Two Models of Induced Diabetes.
IFNgamma suppresses the expression of GFI1 and thereby inhibits Th2 cell proliferation.
Title: IFNγ suppresses the expression of GFI1 and thereby inhibits Th2 cell proliferation.
GFI1 tethers the NuRD complex to open and transcriptionally active chromatin in myeloid progenitors.
Title: GFI1 tethers the NuRD complex to open and transcriptionally active chromatin in myeloid progenitors.
Severe Inflammatory Reactions in Mice Expressing a GFI1(P2A) Mutant Defective in Binding to the Histone Demethylase KDM1A (LSD1).
Title: Severe Inflammatory Reactions in Mice Expressing a GFI1(P2A) Mutant Defective in Binding to the Histone Demethylase KDM1A (LSD1).
PAI-1 is involved in delayed bone repair induced by glucocorticoids in mice.
Title: PAI-1 is involved in delayed bone repair induced by glucocorticoids in mice.
Combinatorial Atoh1 and Gfi1 induction enhances hair cell regeneration in the adult cochlea.
Title: Combinatorial Atoh1 and Gfi1 induction enhances hair cell regeneration in the adult cochlea.

Submit: New GeneRIF Correction See all GeneRIFs (77)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (14) 1 citation
Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AW495828 (e-PCR)
- UniSTS:179223

U78312 (e-PCR)
- UniSTS:163367

Gfi1 (e-PCR), detects polymorphism
- UniSTS:506933

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription repressor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables enzyme-substrate adaptor activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables transcription cis-regulatory region binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of cell fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to lipopolysaccharide	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within inner ear auditory receptor cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within inner ear morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mechanosensory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of NF-kappaB transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of calcidiol 1-monooxygenase activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of cell fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of cell fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of interleukin-6-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of toll-like receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in nuclear matrix	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of transcription repressor complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: zinc finger protein Gfi-1

Names: growth factor independent protein 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001267621.1 → NP_001254550.1 zinc finger protein Gfi-1 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) includes an alternate 5' exon and thus differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (2) has a distinct and longer N-terminus, compared to isoform 1.

Source sequence(s)

AC138666, AV261275

Consensus CDS

CCDS59680.1

UniProtKB/TrEMBL

H3BJM0

Related

ENSMUSP00000135039.3, ENSMUST00000065478.12

Conserved Domains (3) summary

COG5048
Location:321 → 483

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:381 → 401

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam13465
Location:421 → 446

zf-H2C2_2; Zinc-finger double domain
NM_010278.2 → NP_034408.1 zinc finger protein Gfi-1 isoform 1

See identical proteins and their annotated locations for NP_034408.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1).

Source sequence(s)

AC138666, AV261275

Consensus CDS

CCDS57371.1

UniProtKB/TrEMBL

A0A0R4J292

Related

ENSMUSP00000135884.3, ENSMUST00000031205.16

Conserved Domains (3) summary

COG5048
Location:255 → 417

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:315 → 335

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam13465
Location:355 → 380

zf-H2C2_2; Zinc-finger double domain