Men Malic enzyme [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Menprovided by FlyBase
Official Full Name: Malic enzymeprovided by FlyBase
Primary source: FLYBASE:FBgn0002719
Locus tag: Dmel_CG10120
See related: AllianceGenome:FB:FBgn0002719
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-WO0118547.278; CG10120; Dmel\CG10120; MDH; Mdh-NADP; Me; ME; ME1; mem; men; MEN
Summary: Enables malate dehydrogenase (decarboxylating) (NADP+) activity. Involved in several processes, including NADPH regeneration; determination of adult lifespan; and malate metabolic process. Located in cytosol. Is expressed in several structures, including central nervous system; circulatory system; embryonic/larval muscle system; epidermis; and extended germ band embryo. Orthologous to several human genes including ME1 (malic enzyme 1). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 87C6-87C7; 3-52 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (12713103..12722442, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (8538825..8548164, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Regulation at Drosophila's Malic Enzyme highlights the complexity of transvection and its sensitivity to genetic background.
Title: Regulation at Drosophila's Malic Enzyme highlights the complexity of transvection and its sensitivity to genetic background.
these results suggest that metabolic changes mediated by Men during development might be related to the control of ROS tolerance and the longevity of Drosophila.
Title: Overexpression of malic enzyme in the larval stage extends Drosophila lifespan.
trans-interaction between alleles of the Malic enzyme (Men) locus in Drosophila melanogaster
Title: Nonclassical regulation of transcription: interchromosomal interactions at the malic enzyme locus of Drosophila melanogaster.
Studies suggest that variation in levels of cytoplasmic malic enzyme may contribute to variation in alcohol consumption.
Title: Alcohol sensitivity in Drosophila: translational potential of systems genetics.
Malic Enzyme is regulated by juvenile hormone by two mechanisms. One is by a direct effect of JH on the enzyme. The other is by stimulation of transcription of the MEN gene.
Title: Regulation of cytosolic malate dehydrogenase by juvenile hormone in Drosophila melanogaster.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm1718 (e-PCR)
- UniSTS:52954

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables NAD binding	IEA Inferred from Electronic Annotation more info
enables malate dehydrogenase (decarboxylating) (NADP+) activity	IBA Inferred from Biological aspect of Ancestor more info
enables malate dehydrogenase (decarboxylating) (NADP+) activity	IDA Inferred from Direct Assay more info	PubMed
enables malate dehydrogenase (decarboxylating) (NADP+) activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables malic enzyme activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in NADH metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in NADPH regeneration	IDA Inferred from Direct Assay more info	PubMed
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in malate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in malate metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of negative regulation of apoptotic signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pyruvate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in sleep	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	HDA more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: malic enzyme

Names: CG10120-PA; CG10120-PB; CG10120-PC; Men-PA; Men-PB; Men-PC; NADP-dependent malate dehydrogenase; NADP-malic enzyme; malic enzyme (cytosolic)

NP_001262509.1

EC 1.1.1.38

EC 1.1.1.39

EC 1.1.1.40

NP_524880.2

EC 1.1.1.38

EC 1.1.1.39

EC 1.1.1.40

NP_731739.1

EC 1.1.1.38

EC 1.1.1.39

EC 1.1.1.40

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

12713103..12722442 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_080141.4 → NP_524880.2 malic enzyme, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524880.2

Status: REVIEWED

UniProtKB/TrEMBL

Q9NIW2, Q9VG31

Related

FBpp0082129

Conserved Domains (3) summary

cd05312
Location:473 → 750

NAD_bind_1_malic_enz; NAD(P) binding domain of malic enzyme (ME), subgroup 1

PLN03129
Location:213 → 757

PLN03129; NADP-dependent malic enzyme; Provisional

pfam00390
Location:282 → 463

malic; Malic enzyme, N-terminal domain
NM_169479.2 → NP_731739.1 malic enzyme, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_731739.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9NIW1, Q9VG32

Related

FBpp0082130

Conserved Domains (3) summary

cd05312
Location:469 → 746

NAD_bind_1_malic_enz; NAD(P) binding domain of malic enzyme (ME), subgroup 1

PLN03129
Location:209 → 753

PLN03129; NADP-dependent malic enzyme; Provisional

pfam00390
Location:278 → 459

malic; Malic enzyme, N-terminal domain
NM_001275580.1 → NP_001262509.1 malic enzyme, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262509.1

Status: REVIEWED

UniProtKB/TrEMBL

H7BWU6, H7BWU8, Q2QBM1, Q9NIW0

Conserved Domains (3) summary

cd05312
Location:288 → 565

NAD_bind_1_malic_enz; NAD(P) binding domain of malic enzyme (ME), subgroup 1

PLN03129
Location:28 → 572

PLN03129; NADP-dependent malic enzyme; Provisional

pfam00390
Location:97 → 278

malic; Malic enzyme, N-terminal domain