| Context-dependent functional properties of EcR and USP receptors in Drosophila cell survival. | Ligand-independent requirements of steroid receptors EcR and USP for cell survival.
Mansilla A, Martín FA, Martín D, Ferrús A., Free PMC Article | 11/5/2016 |
| nutrition controls ecdysone biosynthesis partially via FoxO-Usp prior to critical weight, ensuring that growth only stops once larvae have achieved a target nutritional status. | Nutritional control of body size through FoxO-Ultraspiracle mediated ecdysone biosynthesis.
Koyama T, Rodrigues MA, Athanasiadis A, Shingleton AW, Mirth CK., Free PMC Article | 07/25/2015 |
| The results suggest that normal larval to pupal maturation in this mecopteran model insect requires the involvement of a distinct endocrine axis of USP binding to its own endogenous terpenoid ligand. | Ligand binding pocket function of Drosophila USP is necessary for metamorphosis.
Jones G, Teal P, Henrich VC, Krzywonos A, Sapa A, Wozniak M, Smolka J, Jones D. | 08/31/2013 |
| PKC mediates USP phosphorylation at Ser35 in Kc and S2 cells, which regulates 20-hydroxyecdysone signaling. | PKC-mediated USP phosphorylation at Ser35 modulates 20-hydroxyecdysone signaling in Drosophila.
Wang S, Wang J, Sun Y, Song Q, Li S. | 05/25/2013 |
| sumoylation of Usp protein can be an important factor in modulating its activity by changing molecular interactions | Alternative sumoylation sites in the Drosophila nuclear receptor Usp.
Bielska K, Seliga J, Wieczorek E, Kędracka-Krok S, Niedenthal R, Ożyhar A. | 01/12/2013 |
| Ecdysone and NO mediate gene regulation by competing EcR/Usp and E75A nuclear receptors during Drosophila development | Ecdysone- and NO-mediated gene regulation by competing EcR/Usp and E75A nuclear receptors during Drosophila development.
Johnston DM, Sedkov Y, Petruk S, Riley KM, Fujioka M, Jaynes JB, Mazo A., Free PMC Article | 12/24/2011 |
| Ecdysone receptor and USP were expressed in numerous adult tissues, but receptor activity varied depending on tissue type and adult age. Receptor activity is reduced when a constitutively inactive ecdysone receptor is present. | Ecdysone receptor expression and activity in adult Drosophila melanogaster.
Schwedes C, Tulsiani S, Carney GE. | 10/29/2011 |
| Data show that ultraspiracle- and ecdysteroid receptor-mediated transcriptional activity not only depends on an interaction between the ecdysteroid receptor and a given hormone response element, but is also modified by surrounding DNA sequences. | The N-terminus of ecdysteroid receptor isoforms and ultraspiracle interacts with different ecdysteroid response elements in a sequence specific manner to modulate transcriptional activity.
Schauer S, Callender J, Henrich VC, Spindler-Barth M. | 05/21/2011 |
| stability of the ecdysone receptor (EcR) is regulated in a cooperative manner by the AB-domains of EcR and Ultraspiracle | Transcriptional activity of ecdysone receptor isoforms is regulated by modulation of receptor stability and interaction with Ab- and C-domains of the heterodimerization partner ultraspiracle.
Ruff H, Tremmel C, Spindler-Barth M. | 02/8/2010 |
| The heterodimerization partner Usp increased DNA binding of ecdysone receptor considerably | DNA-binding properties of Drosophila ecdysone receptor isoforms and their modification by the heterodimerization partner ultraspiracle.
Braun S, Azoitei A, Spindler-Barth M. | 02/8/2010 |
| Results show that three direct targets of ECR/USP--hairy, vrille, and Hr4--are required for cellular differentiation in response to the hormone. | Genomic mapping of binding regions for the Ecdysone receptor protein complex.
Gauhar Z, Sun LV, Hua S, Mason CE, Fuchs F, Li TR, Boutros M, White KP., Free PMC Article | 01/21/2010 |
| heterodimeric complex EcR-B1/Usp mediates the ecdysone-dependent VM32E transcriptional activation in the main body follicle cells. | EcR-B1 and Usp nuclear hormone receptors regulate expression of the VM32E eggshell gene during Drosophila oogenesis.
Bernardi F, Romani P, Tzertzinis G, Gargiulo G, Cavaliere V. | 01/21/2010 |
| A bimolecular fluorescence complementation (BiFC) method was used to directly analyze the formation of the EcR/Usp complex. | The variety of complexes formed by EcR and Usp nuclear receptors in the nuclei of living cells.
Dutko-Gwóźdź J, Gwóźdź T, Orłowski M, Greb-Markiewicz B, Duś D, Dobrucki J, Ozyhar A. | 01/21/2010 |
| results indicate that EcR and Usp can enter the nucleus independently, but cotransport of both receptors mediated by dimerization via the ligand binding domains is possible even in the absence of hormone | Nuclear localization and DNA binding of ecdysone receptor and ultraspiracle.
Cronauer MV, Braun S, Tremmel Ch, Kröncke KD, Spindler-Barth M. | 01/21/2010 |
| data confirm that EcR and Usp can enter the nucleus independently and that intracellular localization is regulated individually for each receptor | Nucleocytoplasmic shuttling of the ecdysteroid receptor (EcR) and of ultraspiracle (Usp) from Drosophila melanogaster in mammalian cells: energy requirement and interaction with exportin.
Betanska K, Nieva C, Spindler-Barth M, Spindler KD. | 01/21/2010 |
| ecdysteroids are capable of inducing heterodimer formation between EcR and USP | Ligand-induced heterodimerization between the ligand binding domains of the Drosophila ecdysteroid receptor and ultraspiracle.
Lezzi M, Bergman T, Henrich VC, Vögtli M, Frömel C, Grebe M, Przibilla S, Spindler-Barth M. | 01/21/2010 |
| USP both represses and activates a gene affecting furrow movement, the ecdysone-responsive Z1 isoform of Broad-Complex, and we report additional usp mutant phenotypes | Analyzing the repressive function of ultraspiracle, the Drosophila RXR, in Drosophila eye development.
Ghbeish N, McKeown M. | 01/21/2010 |
| truncation of its N-terminal A/B region changes molecular characteristics of ultraspiracle, considerably weakening its oligomerization potential under the same experimental conditions | Purification of Drosophila melanogaster ultraspiracle protein and analysis of its A/B region-dependent dimerization behavior in vitro.
Rymarczyk G, Grad I, Rusek A, Oświecimska-Rusin K, Niedziela-Majka A, Kochman M, Ozyhar A. | 01/21/2010 |
| Analysis of the role of ligand-binding domains in the heterodimerization of ecdysone receptor and ultraspiracle protein. | Ligand control of interaction in vivo between ecdysteroid receptor and ultraspiracle ligand-binding domain.
Bergman T, Henrich VC, Schlattner U, Lezzi M., Free PMC Article | 01/21/2010 |
| findings show that the EcR-USP ecdysone receptor is first activated in the extraembryonic amnioserosa, implicating this tissue as a source of active ecdysteroids in the early embryo | Essential roles for ecdysone signaling during Drosophila mid-embryonic development.
Kozlova T, Thummel CS. | 01/21/2010 |
| USP activates transcription and binds ligand with a functional outcome of enhanced promoter activity | Activation of transcription through the ligand-binding pocket of the orphan nuclear receptor ultraspiracle.
Xu Y, Fang F, Chu Y, Jones D, Jones G. | 01/21/2010 |