| Topoisomerase I Essentiality, DnaA-Independent Chromosomal Replication, and Transcription-Replication Conflict in Escherichia coli. | Topoisomerase I Essentiality, DnaA-Independent Chromosomal Replication, and Transcription-Replication Conflict in Escherichia coli.
Leela JK, Raghunathan N, Gowrishankar J., Free PMC Article | 10/23/2021 |
| Supercoiling, R-loops, Replication and the Functions of Bacterial Type 1A Topoisomerases. | Supercoiling, R-loops, Replication and the Functions of Bacterial Type 1A Topoisomerases.
Brochu J, Breton ÉV, Drolet M., Free PMC Article | 02/6/2021 |
| we observe that a single residue substitution (R168C) results in a high rate of head-to-tail (tandem) short sequence duplications, which are independent of existing sequence repeats. Finally, we show that the unique mutation spectrum of topA mutants enhances the emergence of antibiotic resistance in comparison to mismatch-repair (mutS) mutators, and leads to new resistance genotypes. | Point mutations in topoisomerase I alter the mutation spectrum in E. coli and impact the emergence of drug resistance genotypes.
Bachar A, Itzhaki E, Gleizer S, Shamshoom M, Milo R, Antonovsky N., Free PMC Article | 03/21/2020 |
| Study finding that both E. coli topo I and III control R-loop formation/accumulation, mostly to inhibit unregulated replication, may suggest that R-loops have been a problem early in the evolution of life. Furthermore, data show that E. coli cells can survive in the absence of type 1A topos, owing to the surproduction of topo IV that can relax excess negative supercoiling and prevent R-loop formation. | Topoisomerases I and III inhibit R-loop formation to prevent unregulated replication in the chromosomal Ter region of Escherichia coli.
Brochu J, Vlachos-Breton É, Sutherland S, Martel M, Drolet M., Free PMC Article | 01/19/2019 |
| the CyaA(N600Y) mutant has a transcriptome indicative of increased CRP activity, pointing to an unknown role for CyaA and / or cAMP in gene expression..We show that the large scale transcriptomic changes in the topoisomerase I (FusA(A608E)-TopA(S180L)) mutant likely result from increased negative supercoiling in the cell. | Modulation of Global Transcriptional Regulatory Networks as a Strategy for Increasing Kanamycin Resistance of the Translational Elongation Factor-G Mutants in Escherichia coli.
Mogre A, Veetil RT, Seshasayee ASN., Free PMC Article | 07/21/2018 |
| Escherichia coli RecA physically interacts with topoisomerase I to modulate the chromosomal DNA supercoiling. | Investigating direct interaction between Escherichia coli topoisomerase I and RecA.
Banda S, Tiwari PB, Darici Y, Tse-Dinh YC., Free PMC Article | 09/10/2016 |
| Structural study shows how the C-terminal domains of E. coli topoisomerase I bind single-stranded DNA to recognize the accumulation of negative supercoils in duplex DNA. | Structural basis for suppression of hypernegative DNA supercoiling by E. coli topoisomerase I.
Tan K, Zhou Q, Cheng B, Zhang Z, Joachimiak A, Tse-Dinh YC., Free PMC Article | 06/28/2016 |
| Antimicrobial susceptibility and SOS-dependent increase in mutation frequency are impacted by Escherichia coli topoisomerase I C-terminal point mutation. | Antimicrobial Susceptibility and SOS-Dependent Increase in Mutation Frequency Are Impacted by Escherichia coli Topoisomerase I C-Terminal Point Mutation.
Yang J, Annamalai T, Cheng B, Banda S, Tyagi R, Tse-Dinh YC., Free PMC Article | 06/28/2016 |
| Data indicate that transcription-coupled DNA supercoiling (TCDS) in topoisomerase I deficient (topA) is dependent on promoter strength. | Dependence of transcription-coupled DNA supercoiling on promoter strength in Escherichia coli topoisomerase I deficient strains.
Zhi X, Leng F., Free PMC Article | 03/2/2013 |
| Topoisomerase I relaxes DNA in slow processive runs but with short pauses before runs. | Bacterial topoisomerase I and topoisomerase III relax supercoiled DNA via distinct pathways.
Terekhova K, Gunn KH, Marko JF, Mondragón A., Free PMC Article | 01/26/2013 |
| These data show unambiguously that Escherichia coli cells are not viable in the absence of DNA topoisomerase I without the presence of compensatory mutations. | On the viability of Escherichia coli cells lacking DNA topoisomerase I.
Stockum A, Lloyd RG, Rudolph CJ., Free PMC Article | 05/19/2012 |
| The Asp-to-Asn substitutions at the TOPRIM domain residues can selectively decrease Mg(2+) binding affinity with minimal disruption of the active-site geometry, leading to trapping of the covalent complex with cleaved DNA and causing bacterial cell death. | Asp-to-Asn substitution at the first position of the DxD TOPRIM motif of recombinant bacterial topoisomerase I is extremely lethal to E. coli.
Cheng B, Annamalai T, Sorokin E, Abrenica M, Aedo S, Tse-Dinh YC., Free PMC Article | 01/21/2010 |
| DNA of topA null mutants became relaxed rather than hypernegatively supercoiled following depletion of RNase HI activity. | Depletion of RNase HI activity in Escherichia coli lacking DNA topoisomerase I leads to defects in DNA supercoiling and segregation.
Usongo V, Nolent F, Sanscartier P, Tanguay C, Broccoli S, Baaklini I, Drlica K, Drolet M., Free PMC Article | 01/21/2010 |
| N-terminus verified by Edman degradation on mature peptide | Complete nucleotide sequence of the topA gene encoding Escherichia coli DNA topoisomerase I.
Tse-Dinh YC, Wang JC. | 11/5/2007 |
| essential function is related to transcription elongation, to inhibition of R-loop formation and to allowing the synthesis of full-length mRNAs | RNase HI overproduction is required for efficient full-length RNA synthesis in the absence of topoisomerase I in Escherichia coli.
Baaklini I, Hraiky C, Rallu F, Tse-Dinh YC, Drolet M. | 01/21/2010 |
| E. coli strains bearing topA mutations, introduced into the strains by DNA-mediated gene replacement, are viable at 37 or 42 degrees C without any compensatory mutations | Viability of Escherichia coli topA mutants lacking DNA topoisomerase I.
Stupina VA, Wang JC. | 01/21/2010 |
| in response to oxidative stress, Fis binds the promoter region of topA | Differential regulation of Escherichia coli topoisomerase I by Fis.
Weinstein-Fischer D, Altuvia S. | 01/21/2010 |
| analysis of topoisomerase I DNA cleavage activity | Bacterial cell killing mediated by topoisomerase I DNA cleavage activity.
Cheng B, Shukla S, Vasunilashorn S, Mukhopadhyay S, Tse-Dinh YC., Free PMC Article | 01/21/2010 |
| Catalytic roles of the conserved lysine and serine found at the active site of DNA topoisomerase I. | Experimental and computational investigations of Ser10 and Lys13 in the binding and cleavage of DNA substrates by Escherichia coli DNA topoisomerase I.
Strahs D, Zhu CX, Cheng B, Chen J, Tse-Dinh YC., Free PMC Article | 01/21/2010 |