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Platform GPL11012 Query DataSets for GPL11012
Status Public on Mar 09, 2011
Title UvA_MAD-mouse-22K-vs2.0
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Mus musculus
Manufacturer Micro-Array Department, University of Amsterdam
Manufacture protocol 1.Mouse Oligo libraries were bought from Sigma Genosys (Cat # MOULIBST & Cat # MOULIB384B)
2. Resuspend oligos in 25 ul 3 x SSC/1.5 M betain to 20 uM
3. Seal plates with Corning seals.
4. Shake plates near maximum rotational speed on flat-bed shaker for 1 minute.
5. Centrifuge plates at 2000 rpm for 3 minutes.
6. Remove seals and place plate in appropriate location of a Lucidea MicroArrayer (Amersham Pharmacia BioSciences,Piscataway,Nj,USA)
7. Spot library on ultraGAPS slides (Corning)
8. After the print run is completed, allow plates to dry through passive evaporation in a protected environment.
9. Fix oligonucleotides by a heat treatment at 100C for a few seconds. For each subsequent preparation of these plates for a print run, add water to the wells instead of sodium phosphate buffer. The amount of water should be decreased by 0.25 µL per print run, as this is the amount drawn up by the pin capillary.
10. Complete fixation with a UV treatment at 150 mjoule
11. Store arrays at dry and controlled location
 
 
Submission date Oct 04, 2010
Last update date Mar 09, 2011
Contact name Perry D Moerland
E-mail(s) p.d.moerland@amsterdamumc.nl
Organization name Amsterdam UMC
Department Epidemiology and Data Science
Lab Bioinformatics Laboratory
Street address Meibergdreef 9
City Amsterdam
ZIP/Postal code 1105AZ
Country Netherlands
 
Samples (122) GSM603490, GSM603491, GSM603492, GSM603493, GSM603494, GSM603495 
Series (1)
GSE24504 Interorgan coordination of the murine adaptive response to fasting

Data table header descriptions
ID
ENTREZ_ID Entrez Gene ID
UNIGENE_ID UniGene ID
SYMBOL Gene symbol
GENENAME Gene description
COMPUGEN_ID Manufacturer probe ID
FLAG 0 = mouse probe; 1 = control probe
SPOT_LABELS Coordinates of clones on the microarray
GB_ACC GenBank accession number of sequence used to design oligonucleotide probe
SPOT_ID spot id

Data table
ID ENTREZ_ID UNIGENE_ID SYMBOL GENENAME COMPUGEN_ID FLAG SPOT_LABELS GB_ACC SPOT_ID
1 1 R1 - C1 : 1 --Marker
2 1 R1 - C1 : 2 EMPTY
3 1 R1 - C1 : 3 EMPTY
4 1 R1 - C1 : 4 EMPTY
5 1 R1 - C1 : 5 EMPTY
6 1 R1 - C1 : 6 EMPTY
7 1 R1 - C1 : 7 EMPTY
8 1 R1 - C1 : 8 EMPTY
9 1 R1 - C1 : 9 EMPTY
10 1 R1 - C1 : 10 EMPTY
11 1 R1 - C1 : 11 EMPTY
12 1 R1 - C1 : 12 EMPTY
13 1 R1 - C1 : 13 EMPTY
14 1 R1 - C1 : 14 EMPTY
15 1 R1 - C1 : 15 EMPTY
16 1 R1 - C1 : 16 EMPTY
17 17831 Mm.2041 Muc2 mucin 2 CGEN_MOUSE_3000013_1 0 R1 - C1 : 17 AF016695
18 12217 Mm.20425//Mm.437679//Mm.439077 Bsn bassoon CGEN_MOUSE_3000037_1 0 R1 - C1 : 18 NM_007567
19 11853 Mm.262 Rhoc "ras homolog gene family, member C" CGEN_MOUSE_3000061_1 0 R1 - C1 : 19 NM_007484
20 18654 Mm.4809 Pgf placental growth factor CGEN_MOUSE_3000085_1 0 R1 - C1 : 20 NM_008827

Total number of rows: 22680

Table truncated, full table size 2168 Kbytes.






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Supplementary data files not provided
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