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Platform GPL13945 Query DataSets for GPL13945
Status Public on Jul 20, 2011
Title UHNMAC Yeast 6.4 K array (Y6.4K) version 7
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Saccharomyces cerevisiae
Manufacturer Microarray Centre - University Health Network - Toronto - Canada
Manufacture protocol 1. Acquisition of bacterial clone set — our Yeast 6.4k clone set is derived from Research Genetics Genepairs. Detailed information about the ORFs and sequences can be found at http://www.yeastgenome.org/
2. Clone set is PCR amplified and DNA purified in working 96 well plates.
3. The purified DNA is then transferred to 384 well source plates with DNA concentration ranging from 0.10ug/ul to 0.25ug/ul. These are used for spotting.
4. Microarrays are printed on UltraGAPS ™ slides (Corning Inc.) using hihg-precision robotics (up to 48 pins or print heads). Printed arrays are processed following Corning's protocol and are ready-to-use.
Up to 120 slides are printed in each batch and batches that result in greater than 95% printing fidelity are selected for post printing quality control tests
(measuring spot morphology, quality of background and signal intensity in a real hybridization using yeast total RNA, vector probe labeling method and terminal transferase tailing method).
Catalog number UHNMAC Y6.4K
Support glass
Coating amino silane
 
Description spotted DNA/cDNA (double-spotted array containing 6240 yeast ESTs derived from PCR amplification of synthetic EST clones, plus 320 control spots of Arabidopsis totalling 6.4K).
Grid rows: 12; grid columns: 4; rows: 17; columns: 16, grid to grid distance: 4500 um; spot to spot distance: 200 um; spot size: 100 um.
 
Web link http://www.microarrays.ca/
Submission date Jul 19, 2011
Last update date Jul 20, 2011
Contact name Amparo Gamero
E-mail(s) amparo.gamerolluna@gmail.com
Organization name IATA-CSIC
Department Food Biotechnology
Street address Avda. Agustín Escardino 7
City Paterna
State/province Valencia
ZIP/Postal code 46980
Country Spain
 
Samples (54) GSM763603, GSM763604, GSM763605, GSM763606, GSM763607, GSM763608 
Series (8)
GSE30778 Molecular Analysis of the Genes Involved in Aroma Synthesis in the Species S. cerevisiae, S. kudriavzevii and S. bayanus var. uvarum in Winemaking
GSE30779 Genomic and transcriptomic analysis of aroma synthesis in two hybrids between Saccharomyces cerevisiae and S. kudriavzevii in winemaking conditions
GSE46165 Comparative genomic hybridization in traditional fermentative Saccharomyces cerevisiae yeasts

Data table header descriptions
ID
ORF ORF Name
Gene Gene symbols
Component Localization of genes
Function Gene functions
Process Process in which genes are involved
SPOT_ID spot id

Data table
ID ORF Gene Component Function Process SPOT_ID
3XSSC --3XSSC
Arabidopsis --Arabidopsis
YAL001C YAL001C TFC3 transcription factor TFIIIC complex RNA polymerase III transcription factor activity transcription initiation from Pol III promoter
YAL002W YAL002W VPS8 membrane fraction molecular_function unknown late endosome to vacuole transport
YAL003W YAL003W EFB1 ribosome translation elongation factor activity translational elongation
YAL004W YAL004W cellular_component unknown molecular_function unknown biological_process unknown
YAL005C YAL005C SSA1 cytoplasm; cell wall (sensu Fungi); nucleus; vacuolar membrane (sensu Fungi) chaperone activity; ATPase activity; heat shock protein activity "SRP-dependent cotranslational membrane targeting, translocation; protein-nucleus import, translocation; protein folding"
YAL007C YAL007C ERP2 COPII-coated vesicle molecular_function unknown ER to Golgi transport
YAL008W YAL008W FUN14 mitochondrion molecular_function unknown biological_process unknown
YAL009W YAL009W SPO7 integral to membrane; nuclear envelope-endoplasmic reticulum network molecular_function unknown sporulation (sensu Saccharomyces); nuclear organization and biogenesis; meiosis
YAL010C YAL010C MDM10 mitochondrial outer membrane molecular_function unknown mitochondrion organization and biogenesis; mitochondrial genome maintenance; mitochondrion inheritance
YAL011W YAL011W SWC1 nucleus molecular_function unknown biological_process unknown
YAL012W YAL012W CYS3 cytoplasm cystathionine gamma-lyase activity cysteine metabolism; sulfur amino acid metabolism; transsulfuration
YAL013W YAL013W DEP1 cellular_component unknown molecular_function unknown regulation of transcription from Pol II promoter; phospholipid metabolism
YAL014C YAL014C SYN8 endosome SNAP receptor activity transport
YAL015C YAL015C NTG1 mitochondrion; nucleus purine-specific oxidized base lesion DNA N-glycosylase activity; pyrimidine-specific oxidized base lesion DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity "DNA repair; base-excision repair, AP site formation; base-excision repair"
YAL016W YAL016W TPD3 protein phosphatase type 2A complex; spindle pole body; bud tip; shmoo tip; cytoplasm; nucleus; bud neck protein phosphatase type 2A activity protein biosynthesis; protein amino acid dephosphorylation; bud growth; mitotic spindle checkpoint; actin filament organization
YAL017W YAL017W PSK1 cytoplasm protein serine/threonine kinase activity protein amino acid phosphorylation; hexose metabolism
YAL018C YAL018C YAL018C cellular_component unknown molecular_function unknown biological_process unknown
YAL019W YAL019W FUN30 nucleus molecular_function unknown chromosome organization and biogenesis (sensu Eukarya)

Total number of rows: 6191

Table truncated, full table size 743 Kbytes.




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