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Platform GPL15371 Query DataSets for GPL15371
Status Public on Mar 23, 2012
Title PLBIO-DX - WH/GEX
Technology type spotted oligonucleotide
Distribution custom-commercial
Organism Homo sapiens
Manufacturer Biomarker Discovery, Translational Research, Piramal Healthcare India Limited
Manufacture protocol A microarray chip with 37,632 transcripts representing the whole human genome were purchased from Operon Biotechnologies (Operon, USA). Every gene was confirmed for function and sequence with the latest RefSeq genome build and represented as a unique 70-mer oligonucleotides sequence. A 40μM concentration of the oligos was achieved by dissolving the oligos with 50% DMSO solution. The MTP plates were vortexed at 150 rpm for 2 hours and spun down at 1200 rpm in a swing out rotor centrifuge. Every gene/oligo was printed with a robotic arrayer – OG100 (Genomic Solutions, Ann Arbor, USA) on aminosilane coated glass slide (ArrayIT, USA). The relative humidity and the temperature were maintained at 45% RH and 23°C. The printing is achieved by contact disposition of 20nL of the oligo suspension onto the glass slide with stealth microarray pins (SMP3) having an uptake capacity of 250nL. Post printing, the slides were air dried for 6 hours in the printing environment and then cross-linked at 650 mL/sec2 with an UV cross-linker. The slides were then packed and stored under vacuum in the dark at room temperature.
 
 
Submission date Mar 22, 2012
Last update date Mar 23, 2012
Contact name Muralidhara Padigaru
E-mail(s) muralidhara.padigaru@piramal.com
Phone +912230818802
Organization name Piramal Lifesciences India Limited
Department Translational Research
Lab Biomarker Discovery
Street address 1, Nirlon Compex, Off Western Express Highway
City Mumbai
State/province Maharashtra
ZIP/Postal code 400063
Country India
 
Samples (14) GSM899255, GSM899256, GSM899257, GSM899258, GSM899259, GSM899260 
Series (1)
GSE36703 CDK-4 inhibitor P276-00 sensitizes Pancreatic Cancer cells to Gemcitabine induced Apoptosis

Data table header descriptions
ID
Spot_address
Probe_ID
Gene Symbol
ORF ORF Name
SEQUENCE
Gene name
SPOT_ID

Data table
ID Spot_address Probe_ID Gene Symbol ORF SEQUENCE Gene name SPOT_ID
PL_1 A1.a1 H200000163 NDUFB7 NDUFB7 ATGTGATGCGCATGAAGGAGTTTGAGCGGGAGCGGAGGCTGCTCCAGCGGAAGAAGCGGCGGGAGAAGA NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (EC 1.6.5.3) (EC 1.6.99.3) (NADH-ubiquinone oxidoreductase B18 subunit) (Complex I-B18) (CI-B18) (Cell adhesion protein SQM1). [Source:Uniprot/SWISSPROT;Acc:P17568]
PL_2 A1.a10 H200000796 FUSIP1 FUSIP1 GCTTTTCACACCTCAGTTTATTTAATACGAGGCAAGTTGTAAGACAACACTCATTCTAGGTGATTCTGT FUS-interacting serine-arginine-rich protein 1 (TLS-associated protein with Ser-Arg repeats) (TLS-associated protein with SR repeats) (TASR) (TLS-associated serine-arginine protein) (TLS-associated SR protein) (40 kDa SR-repressor protein) (SRrp40) (Splic [Source:Uniprot/SWISSPROT;Acc:O75494]
PL_3 A1.a11 H200000825 COPS7A COPS7A AGCATCTGTCCCTGTTCATTACATGTCATTGAGTAGGTGGGTAGCCCTGATGGGGGTCGCTCTGTCTGG COP9 signalosome complex subunit 7a (Signalosome subunit 7a) (SGN7a) (JAB1-containing signalosome subunit 7a) (Dermal papilla-derived protein 10). [Source:Uniprot/SWISSPROT;Acc:Q9UBW8]
PL_4 A1.a12 H200000838 STMN4 STMN4 ACATGTTAAGAATTGACCAGTGAAGCCATCCTATTTGTTTCCGGGGAACAATGACGGGGTGGGAGAGGG Stathmin-4 (Stathmin-like protein B3) (RB3). [Source:Uniprot/SWISSPROT;Acc:Q9H169]
PL_5 A1.a13 H200000870 THRAP2 THRAP2 ACCATGGTTTGAACAAAAAAAGGGGAAATGGGGAGGGGAAAAGGGTGGGATTGTCCAGCATGCTTGTAT Thyroid hormone receptor-associated protein 2 (Thyroid hormone receptor-associated protein complex 240 kDa component-like). [Source:Uniprot/SWISSPROT;Acc:Q71F56]
PL_6 A1.a14 H200000879 REG1B REG1B AAATTAAACTAGACTATGTCTCCAACTCAGTTCAGACCATCTCCTCCCTAATGAGTTTGCATCGCTGAT Lithostathine 1 beta precursor (Regenerating protein I beta). [Source:Uniprot/SWISSPROT;Acc:P48304]
PL_7 A1.a15 H200000913 PSMC1 PSMC1 ATGTTCTTTATAAGAAACAGGAAGGCACCCCTGAGGGGCTGTATCTCTAATGAACCATGGCTGTCATCA 26S protease regulatory subunit 4 (P26s4) (Proteasome 26S subunit ATPase 1). [Source:Uniprot/SWISSPROT;Acc:P62191]
PL_8 A1.a16 H200000919 FEN1 FEN1 CCTTAAGAGCTACAGCTAGAGAAACCTTCACGGGGTGGAGAGAGGATTCTAAGGCTTTTCTAGCGTGAC Flap endonuclease 1 (EC 3.1.-.-) (Flap structure-specific endonuclease 1) (FEN-1) (Maturation factor 1) (MF1) (hFEN-1) (DNase IV). [Source:Uniprot/SWISSPROT;Acc:P39748]
PL_9 A1.a17 H200001462 C12orf29 C12orf29 AGTGATGGCTGTTTAATAAAGGTCCATCGCCATCATCTTGGCTTATGCTGGCCAATTCCAGATACTTAC C12orf29 protein (Fragment). [Source:Uniprot/SPTREMBL;Acc:Q8IYQ5]
PL_10 A1.a18 H200001474 GABARAP GABARAP AGGCTCCCAAAGCTCGGATAGGAGACCTGGACAAAAAGAAATACCTGGTGCCTTCTGATCTCACAGTTG Gamma-aminobutyric acid receptor-associated protein (GABA(A) receptor- associated protein) (MM46). [Source:Uniprot/SWISSPROT;Acc:O95166]
PL_11 A1.a19 H200001516 PELI1 PELI1 GGGTTGAATGACAATAGTGCCCCCGTTTTTAGCAGACCAGCCTTAACTCTGGGTTTGAATCCTAAGGAG Protein pellino homolog 1 (Pellino-1) (Pellino-related intracellular- signaling molecule). [Source:Uniprot/SWISSPROT;Acc:Q96FA3]
PL_12 A1.a2 H200000170 CSTF2 CSTF2 GGGGAGGCACTTTACTTTCTGTAACTGGAGAGGTAGAGCCTAGAGGTTACTTGGGACCACCTCATCAGG Cleavage stimulation factor 64 kDa subunit (CSTF 64 kDa subunit) (CF-1 64 kDa subunit) (CstF-64). [Source:Uniprot/SWISSPROT;Acc:P33240]
PL_13 A1.a20 H200001529 SCAMP5 SCAMP5 CAAAAATCCAAACCCCACTTGGCTACTCTGGCCTGGCTTCAGCTTGGAACCCAATACCTAGGCTTACAG Secretory carrier-associated membrane protein 5 (Secretory carrier membrane protein 5). [Source:Uniprot/SWISSPROT;Acc:Q8TAC9]
PL_14 A1.a21 H200001559 SLC25A38 SLC25A38 CGAGCCCTCCGCAGAACTCTAATGGCAGCAATGGCGTGGACGGTGTATGAAGAGATGATGGCCAAGATG "solute carrier family 25, member 38 [Source:RefSeq_peptide;Acc:NP_060345]"
PL_15 A1.a22 H200001569 RSAD1 RSAD1 CTACAAAACCTCATGCCTACTGTCTGCCACTGCTGGAGAAGGAATCATGGTTTCTAGAAGTGCCTCTCA radical S-adenosyl methionine domain containing 1 [Source:RefSeq_peptide;Acc:NP_060816]
PL_16 A1.a23 H200001599 GCATGCACCCACATGCCAATTTTTATTGCTTAAAATGGCGAGTTCTATGGATATTATCTAGCCCCAAGC -
PL_17 A1.a24 H200001606 TLOC1 TLOC1 GAACAGCATTTGTTGGAAGGTGATGGCTCTCCCTCCTCCCTCCCCATTTCATTGGCGTAACGTAAAGTG Translocation protein SEC62 (Translocation protein 1) (TP-1) (hTP-1). [Source:Uniprot/SWISSPROT;Acc:Q99442]
PL_18 A1.a25 H200002170 PLLP PLLP TATTCAGTGGAAGATGACGGGGGATCTGAGGCTGTGTCTCTGCCTTGTCTTTAGAGGACTTCAGCGTCC Plasmolipin (Plasma membrane proteolipid). [Source:Uniprot/SWISSPROT;Acc:Q9Y342]
PL_19 A1.a26 H200002183 Q5JP46_HUMAN Q5JP46_HUMAN GGGGGACTATATCCGTGTACGCAAGGCCATCACTGCTGGTTACTTTTACCACACGGCACGGTTGACTCG DHX16 protein (Fragment). [Source:Uniprot/SPTREMBL;Acc:Q6PKC8]
PL_20 A1.a27 H200002213 Q3MJC0_HUMAN Q3MJC0_HUMAN CATTTCGCGGAACCACGTGACGCGACTGCTTCAGAACTATAAGAAACAGCCTCGGAATTCTATGATTAA -

Total number of rows: 37632

Table truncated, full table size 6476 Kbytes.




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