University Health Network Clinical Genomics Centre Microarray Centre
Manufacture protocol
1. Acquisition of bacterial clone set — our Yeast 6.4k clone set is derived from Research Genetics Genepairs, Detailed information about the ORFs and sequences can be found at http://www.yeastgenome.org/ 2. Clone set is PCR amplified and DNA purified in working 96 well plates. 3. The purified DNA is then transferred to 384 well source plates with DNA concentration ranging from .10ug/ul to 0.25ug/ul. These are used for spotting. We use high precision robots that can use up to 48 pins or print heads. The Y6.4kv6 slides are printed using 48 pins and we use UltraGAPS ™ slides (Corning Inc.). We are able to print up to 120 slides in each batch and batches that result in greater than 95% printing fidelity are selected for post printing quality control tests.
Catalog number
Yeast 6.4kv6
Support
glass
Coating
aminosilane
Description
Grid rows-12; Grid columns-4; Rows-20; Columns-20; Grid to grid distance-4500um; Spot to spot distance-200um; Spot size-100um.
Expression patterns in stress conditions of 4 closely related yeast species from the Saccharomyces sensu stricto complex
Data table header descriptions
ID
Name
Original_Gene:Short Description:Current_ORF:Flag:UND_ID. Original_Gene – Gene used to identify the original Yeast ORF. Short Description – Description based on the original Yeast ORF. Current_ORF – New Yeast ORF name given to clone based on either just a name change or merger of the original ORF. Flag – the flag distinguishes any changes made to the Yeast ORF: 1-No changes; Original_ORF used; 5-Sequence change only; Original_ORF used; 10- Yeast ORF deleted from SGD database; Original_ORF used for reference; 100- Either name change or merge with another; Current_ORF used. UHN_ID – These are IDs assigned by the UHN Microarray Centre for clone identification purposes.
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