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Status
Public on Aug 26, 2008
Title
Earthworm 4K cDNA array version 1.1
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Eisenia fetida
Manufacturer
USACE-ERDC-EL Environmental Genomics and Genetics team
Manufacture protocol
1. A total of 4032 purified cDNA clones were loaded on 384-well plates and completely dried down in a Vacufuge Concentrator 5301 (Eppendorf, Westbury, NY). 2. The dried cDNA was re-suspended in 15ul of 1¡Á printing buffer (ArrayIt, Sunnyvale, CA). 3. Each clone was spotted twice (i.e., in two super grids) on Ultra GAPS amino silane coated glass slides (Corning, Acton, MA) using 16 pins on a VersArray ChipWriter (Bio-Rad, Hercules, CA). 4. Five alien cDNAs, i.e., PCR product 1 to 5 selected from SpotReport Alien cDNA Array Validation System (Stratagene, La Jolla, CA) prepared at 15, 30, 60, 125 and 250 ng/ul were also spotted twice along with printing buffer and water as control spots. 5. The total number of spots on each array was 8704 including 60 alien cDNA spots, 84 water spots, 256 blank spots and 240 printing buffer spots. 6. After printing, arrays were incubated in a dessicator 2-3 days and were then snap-dried on a hot plate after being rehydrated over a boiling water bath. 7. The arrays were further immobilized using a UV Cross-linker (Stratagene) by applying 300 mJ per 10 arrays. 8. Printing time: May 12, 2006
Support
Corning Ultra GAPS coated glass slide
Coating
gamma amino propyl silane (GAPS)
Contributor(s)
Gong P , Guan X , Pirooznia M , Indest KJ , Perkins EJ
Submission date
Aug 27, 2007
Last update date
Aug 26, 2008
Contact name
Ping Gong
E-mail(s)
ping.gong@us.army.mil
Phone
(601) 634-3521
Organization name
US Army ERDC
Department
Environmental Laboratory
Lab
Environmental Genomics and Genetics
Street address
3909 Halls Ferry Road
City
Vicksburg
State/province
MS
ZIP/Postal code
39180
Country
USA
Samples (40)
GSM225315 , GSM225321 , GSM225340 , GSM225341 , GSM225342 , GSM225343
GSM225344 ,
GSM225345 ,
GSM225346 ,
GSM225352 ,
GSM225359 ,
GSM225525 ,
GSM225531 ,
GSM225536 ,
GSM225550 ,
GSM225559 ,
GSM225570 ,
GSM225581 ,
GSM225588 ,
GSM225592 ,
GSM225597 ,
GSM225602 ,
GSM225605 ,
GSM225617 ,
GSM225620 ,
GSM225621 ,
GSM225623 ,
GSM225624 ,
GSM225625 ,
GSM225626 ,
GSM225627 ,
GSM225628 ,
GSM225629 ,
GSM225638 ,
GSM225639 ,
GSM225640 ,
GSM225643 ,
GSM225644 ,
GSM225646 ,
GSM225650
Series (1)
GSE8909
Profiling differentially expressed earthworm genes in response to TNT, RDX, or TNT+RDX exposure.
Data table header descriptions
ID
Spot number
dbEST_ID
GenBank EST database ID number
CLONE ID
GB_ACC
GenBank accession number of spotted cDNA
Contig_ID
Assembled contig number
Hit_GB_Acc
GenBank matching protein accession number
E_value
Expectation value for matching protein
Gen_Desc
Putative gene function description
Super_Grid
Duplicated super grid number
Array_Grid
Grid number on array
Array_Row
Row number on array
Array_Col
Column number on array
Spot_Loc
Spot position (Grid-Row:Column)
SPOT_ID
controls
Data table
ID
dbEST_ID
CLONE ID
GB_ACC
Contig_ID
Hit_GB_Acc
E_value
Gen_Desc
Super_Grid
Array_Grid
Array_Row
Array_Col
Spot_Loc
SPOT_ID
1
EW1_R1P07_H05
1
1
1
1
1-1:1
ambiguous clone_EW1_R1P07_H05
2
44048070
EW1_R1P05_G01
EH671164
AAD30302.1
2.1
urokinase plasminogen activator receptor
1
1
1
2
1-1:2
3
44047882
EW1_R1P02_G09
EH670976
BAD91025.1
3E-61
valosin containing protein-2
1
1
1
3
1-1:3
4
44047011
EW1_F1P09_H05
EH670105
XP_694466.1
0.0003
PREDICTED: similar to RIKEN cDNA 4930429A22
1
1
1
4
1-1:4
5
44904747
EW1_F2P15_G01
EL515452
P81695
6E-22
MT_EISFO Cadmium-metallothionein (MT)
1
1
1
5
1-1:5
6
44047236
EW1_F2P12_G09
EH670330
Contig209
******No hits
1
1
1
6
1-1:6
7
44046848
EW1_F1P07_H05
EH669942
Contig132
CAA58705.1
1E-34
intermediate filament protein
1
1
1
7
1-1:7
8
44046671
EW1_F1P05_G01
EH669765
Contig166
NP_001033116.1
0.000000002
glutamyl aminopeptidase (aminopeptidase A)
1
1
1
8
1-1:8
9
44046426
EW1_F1P02_G09
EH669520
NP_001006352.1
0.00000002
ADP-ribosylation factor 1
1
1
1
9
1-1:9
10
44049270
EW2_R1P11_H05
EH672364
AAA67037.1
4E-25
protein kinase
1
1
1
10
1-1:10
11
44049100
EW2_R1P09_G01
EH672194
AAH90516.1
4E-20
LOC553323 protein
1
1
1
11
1-1:11
12
44048891
EW2_R1P06_G09
EH671985
Contig424
CAE18118.1
2E-15
SCBP3 protein
1
1
1
12
1-1:12
13
44048651
EW2_R1P03_H05
EH671745
XP_953940.1
1.7
hypothetical protein TA05930
1
1
1
13
1-1:13
14
44048507
EW2_R1P01_G01
EH671601
XP_653341.1
3.6
receptor protein kinase
1
1
1
14
1-1:14
15
EW2_F1P01_G09
1
1
1
15
1-1:15
ambiguous clone_EW2_F1P01_G09
16
44047587
EW1_F1P18_A08
EH670681
Contig447
******No hits
1
1
1
16
1-1:16
17
44047692
EW1_F1P20_B12
EH670786
AAH47987.1
4.8
Dj462o23.2-prov protein
1
1
1
17
1-1:17
18
44048178
EW1_R1P07_H09
EH671272
AAH59622.1
6E-40
Phosphopantothenoylcysteine decarboxylase
1
1
2
1
1-2:1
19
44048074
EW1_R1P05_G05
EH671168
XP_396744.2
1E-27
PREDICTED: similar to oocyte membrane protein
1
1
2
2
1-2:2
20
EW1_R1P02_H01
1
1
2
3
1-2:3
ambiguous clone_EW1_R1P02_H01
Total number of rows: 8704 Table truncated, full table size 778 Kbytes .
Supplementary data files not provided