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Platform GPL6647 Query DataSets for GPL6647
Status Public on Oct 10, 2008
Title Micro-CRIBI Human Oligo Array V_0 (Operon V2.0)
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Homo sapiens
Manufacturer CRIBI University of Padova Microarray facility (http://microcribi.cribi.unipd.it)
Manufacture protocol 1.Oligos are arrayed in Genetix 384 Well Microarray Plates (Cat# X7020). Each well contains 600 pmol of 70-mer oligo.
2. Resuspend oligos in 17 ┬ÁL of Telechem Microspotting solution 1X.
3. Seal plates with Corning seals and after oligos resuspenction print probe with the MicroGrid II Biorobotics spotter.
4. Print is performed with 48 Stealth pins.
5. After printing run plates are stored at -20 C degree.
6. Every 4 months plates are lyophilized and resuspended in adjusted water volume according to the number of spotting runs. This, to maintain the same volume and concentration in all plate well.
Support glass
Coating aminosilane
 
Description This set consists of 21,329 70mer oligonucleotides (Operon version 2.0) designed on Human Unigene clusters, mainly in the 3 end terminal region. Each oligonucleotide is spotted by our robotic station Biorobotics Microgrid II, in one replicate on a MICROMAX glass slide SuperChip I (Cat No MPS696) provided by PerkinElmer Life Sciences Inc. The deposition is qualitatively assessed by a scan of the spotted slide at 10 micron resolution, using a GSI Lumonics scanner and a protocol that allows the detection of spotted features through the salts derived from the dried solution in which oligonucleotides are dissolved. Moreover all spotted batch are evaluated for the spot shape and oligo linking onto the slide surface using the vistra green method. Microarray slides that meet the quality standards, are washed as recommended by the supplier and the glass slides are stored, ready for use, in the dark under deep vacuum.
 
Web link http://microcribi.cribi.unipd.it
Contributor(s) Cagnin S, Pacchioni B, Millino C, Celegato B, De Pitta' C, Tombolan L, Romualdi C, Lanfranchi G
Submission date Mar 25, 2008
Last update date Oct 10, 2008
Contact name Gerolamo Lanfranchi
E-mail(s) stefano.cagnin@unipd.it
Phone +39-0498276219
Organization name University of Padova
Department CRIBI - Biotechnology Center and Biology Department
Lab Functional Genomics Lab
Street address Via U. Bassi, 58/B
City Padova
ZIP/Postal code 35131
Country Italy
 
Samples (66) GSM279949, GSM279950, GSM279951, GSM279952, GSM279953, GSM279954 
Series (1)
GSE11138 LAD coronary atherosclerotic plaque gene expression

Data table header descriptions
ID
ID_Operon_Oligo Oligo Identify code by Operon Human V2.0 (https://www.operon.com/arrays/human_V2_384.prn)
GB_ACC GenBank Accession number
Description Oligo probe description
UniGene_ID UniGene accession number
GENE Entrez GENE ID
Gene_Symbol Official gene symbol
Array.Row Array row position in CRIBI human oligo array V_0 (Operon V2.0)
Array.Column Array column position in CRIBI human oligo array V_0 (Operon V2.0)
Spot.Row Row probe position in sub-array
Spot.Column Column probe position in sub-array
SPOT_ID

Data table
ID ID_Operon_Oligo GB_ACC Description UniGene_ID GENE Gene_Symbol Array.Row Array.Column Spot.Row Spot.Column SPOT_ID
1 H200000100 NM_000911 001A12_Opioid receptor. delta 1 372 4985 OPRD1 1 1 1 1
2 H200000124 NM_004024 001E12_Activating transcription factor 3 460 467 ATF3 1 1 1 2
3 H200000148 NM_000384 001I12_Apolipoprotein B (including Ag(x) antigen) 585 338 APOB 1 1 1 3
4 H200000172 NM_000100 001M12_Cystatin B (stefin B) 695 1476 CSTB 1 1 1 4
5 H200000106 NM_001124 001A24_Adrenomedullin 394 133 ADM 1 1 1 5
6 H200000130 NM_002202 001E24_ISL1 transcription factor. LIM/homeodomain. (islet-1) 505 3670 ISL1 1 1 1 6
7 H200000154 NM_002306 001I24_Lectin. galactoside-binding. soluble. 3 (galectin 3) 621 3958 LGALS3 1 1 1 7
8 H200000178 NM_003250 001M24_Thyroid hormone receptor. alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog. avian) 724 7067 THRA 1 1 1 8
9 H200000480 NM_000277 002A12_Phenylalanine hydroxylase 1870 5053 PAH 1 1 1 9
10 H200000504 NM_000180 002E12_Guanylate cyclase 2D. membrane (retina-specific) 1974 3000 GUCY2D 1 1 1 10
11 H200000528 NM_000373 002I12_Uridine monophosphate synthetase (orotate phosphoribosyl transferase and orotidine-5'-decarboxylase) 2057 7372 UMPS 1 1 1 11
12 H200000552 NM_000760 002M12_Colony stimulating factor 3 receptor (granulocyte) 2175 1441 CSF3R 1 1 1 12
13 H200000486 NM_000892 002A24_Kallikrein B. plasma (Fletcher factor) 1 1901 3818 KLKB1 1 1 1 13
14 H200000510 NM_003140 002E24_Sex determining region Y 1992 6736 SRY 1 1 1 14
15 H200000534 NM_000956 002I24_Prostaglandin E receptor 2 (subtype EP2). 53kD 2090 5732 PTGER2 1 1 1 15
16 H200000558 NM_006685 002M24_Proline rich 3 2207 10879 PROL3 1 1 1 16
17 H200000860 NM_014403 003A12_Sialyltransferase 7D ((alpha-N-acetylneuraminyl-2.3-beta-galactosyl-1.3)-N-acetyl galactosaminide al 3972 27090 SIAT7D 1 1 1 17
18 H200000884 AK024956 003E12_Homo sapiens cDNA: FLJ21303 fis. clone COL02107 4194 1 1 1 18
19 H200000908 NM_001412 003I12_Eukaryotic translation initiation factor 1A 4310 1964 EIF1A 1 1 1 19
20 H200000932 NM_006703 003M12_Nudix (nucleoside diphosphate linked moiety X)-type motif 3 4815 11165 NUDT3 1 1 1 20

Total number of rows: 21888

Table truncated, full table size 2144 Kbytes.






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