NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Platform GPL8599 Query DataSets for GPL8599
Status Public on May 31, 2010
Title BMFZ Mouse 40K V.1 part 1
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Mus musculus
Manufacturer BMFZ Heinrich Heine University Düsseldorf
Manufacture protocol 1. Oligos are purchased from Operon (AROS Mus Musculus V.4.0, plate 1-48 + control plate and buffer plate) arrayed in Genetix V-bottom 384-well plates. Each well contains 300 pmol of 70-mer oligo.
2. A day before printing oligos were resuspend in 15 µl Amino-Spotting-Buffer ((Genetix Ltd., Hampshire, UK)
3. Centrifuge plates at 1000 rpm for 5 minutes.
4. Plates were sealed and store at 8°C over night
5. Centrifuge plates at 1000 rpm for 5 minutes.
6. Remove seals and cover with plate lids. Place in appropriate location of plate cassette.
7. After the print run is completed, allow plates to dry through passive evaporation in a protected environment.
8. For each subsequent preparation of these plates for a print run, add water to the wells instead of spotting buffer. The amount of water should be decreased by 0.5 µL per print run, as this is the amount drawn up by the pin capillary during each print run.
Support UltraGap slides (Corning Inc., NY, U.S.A.).
Coating Aminosilane
 
Description spotter = QArray (Genetix)
 
Contributor(s) Mehrens J, Köhrer K
Submission date May 27, 2009
Last update date May 31, 2010
Contact name Antje Lindecke
E-mail(s) antje.lindecke@uni-duesseldorf.de
Phone 49-211-8114367
Organization name Universitaet Duesseldorf
Department BMFZ
Lab Microarray
Street address Universitaetsstr. 1
City Duesseldorf
ZIP/Postal code 40225
Country Germany
 
Samples (16) GSM409037, GSM409038, GSM409039, GSM409040, GSM409041, GSM409042 
Series (1)
GSE16243 Analysis of cardiac gene expression in response to isoproterenol-induced heart failure (part 1)

Data table header descriptions
ID
GENE SYMBOL
DESCRIPTION
ENSEMBL GENE ID
GB_LIST
SEQUENCE
SPOT_ID

Data table
ID GENE SYMBOL DESCRIPTION ENSEMBL GENE ID GB_LIST SEQUENCE SPOT_ID
1 BLANK
2 Hoxa10 Homeobox protein Hox-A10 (Hox-1.8). [Source:Uniprot/SWISSPROT;Acc:P31310] ENSMUSG00000000938 AC113985, BC050839, AC015583 TGGAAGCCTAGGTGGGCTGGGGCAAGCAGAAATAAAAATGAGAGAAGGGAGATATTGTTTGGATTTCCT
3 Vim Vimentin. [Source:Uniprot/SWISSPROT;Acc:P20152] ENSMUSG00000026728 BC089335 GCAGGATTTCTCTGCCTCTGCCAACCTTTTCTTCCCTGAACCTGAGAGAAACTAACCTGGAGTCACTTC
4 Srm Spermidine synthase (EC 2.5.1.16) (Putrescine aminopropyltransferase) (SPDSY). [Source:Uniprot/SWISSPROT;Acc:Q64674] ENSMUSG00000006442 BC005566, AL606969, AL591032 GACCAAGGATCTTCCAAGTTGTCTGGGGACCACCAGTCCTGGACCAGACTCCCAGATGACTCTTGCCCA
5 Myf6 Myogenic factor 6 (Myf-6) (Herculin). [Source:Uniprot/SWISSPROT;Acc:P15375] ENSMUSG00000035923 GCCCCCAGTGGCCAAGTGTTTCGGATCATTCCAGGGGCCTCGTGATAACTGCTAAGGAAGGAGGAGCAA ENSMUSG00000035923
6 Bcap31 B-cell receptor-associated protein 31 (BCR-associated protein Bap31) (p28 Bap31). [Source:Uniprot/SWISSPROT;Acc:Q61335] ENSMUSG00000002015 BC002106, AC091453, AL805924, AF459436 CTGGTGACCTGGCCTGCAGTTTGAACCCCTTTTTATTTTGCAATGTACCCTAAAGGAGGCTGCTGTGAA
7 SEP1_MOUSE Septin 1 (Differentiation protein 6) (Diff6 protein). [Source:Uniprot/SWISSPROT;Acc:P42209] ENSMUSG00000000486 AC133494, AC122537 AGAAAGTCAATATCATCCCGGTCATCGGTAAAGCAGACGCCCTGATGCCTAGGGAGACTCAGGCCCTCA
8 ENSMUSG00000037482 BC099428, AL833794 GTATATGAGCAAACTCGCTCAGACTGTAAGGACCCAAATTATGGAGGATGTCCACACTGGGGCTGCAAA
9 Hnrpab Heterogeneous nuclear ribonucleoprotein A/B (hnRNP A/B) (CArG-binding factor-A) (CBF-A). [Source:Uniprot/SWISSPROT;Acc:Q99020] ENSMUSG00000020358 AC105470, BC066664, BC043069, AL645602, AF108653 GCCGAGTGACGACCGCACACGCTTTGTTTGGACGCGGAGTGAACACAATTATGTACCAAATTTAACTTG
10 Gas2 Growth-arrest-specific protein 2 (GAS-2). [Source:Uniprot/SWISSPROT;Acc:P11862] ENSMUSG00000030498 AC122017, AC113312, BC053446, BC013456 CCTGTGCAGCCCCTTAGGAATAGCAGGTACCTTTTACACACAGAGAAGCTGTGTTTAGGATGATCACTT
11 Bcl7b B-cell CLL/lymphoma 7B. [Source:RefSeq;Acc:NM_009745] ENSMUSG00000029681 AC024607 ATGGAGGGCGTGGGGTCGTCGCACCATCTTCTTGTTCCTGACACTTGTGTCTGCTTGCTCTTGAGCAAA
12 Cops3 COP9 (constitutive photomorphogenic), subunit 3; COP9 (constitutive photomorphogenic), subunit 3 (Arabidopsis); COP9 complex S3. [Source:RefSeq;Acc:NM_011991] ENSMUSG00000019373 BC068179, AC068808, AF071313 AGCCTCTCAGCAATGCTTACCATGAGTTAGCACAAGTTTATTCAACCAACAACCCGTCTGAGCTGCGGA
13 Myl4 Myosin light chain 1, atrial/fetal isoform (MLC1A) (MLC1EMB). [Source:Uniprot/SWISSPROT;Acc:P09541] ENSMUSG00000061086 BC048485 AGCCCCTCAAGGACTCTGCCTTTGACCCAAAGAGTGTGAAGATAGACTTCAGTGCTGACCAGATCGAAG
14 D11Ertd99e ENSMUSG00000017188 BC026206, AL590969 CCTAACTGTATGGACCATCTTCAAACTGCTGGATCCCCTGTATATGTTGAATGATGCCTGGTGGTCTTA
15 Trim27 Zinc-finger protein RFP (Ret finger protein) (Tripartite motif protein 27). [Source:Uniprot/SWISSPROT;Acc:Q62158] ENSMUSG00000021326 BC085503, BC069924, BC003219, AC138330 ATGCCATACCTTTACTTTCTCTCACGCTACCTTCTGTGGGCCAGTCCGGCCCTACTTCAGCCTAAGCTA
16 Acr Acrosin precursor (EC 3.4.21.10). [Source:Uniprot/SWISSPROT;Acc:P23578] ENSMUSG00000022622 AC122401, BC103580, BC103579, BC103578, BC103577 AACGTAGTGACGGAGGGAAATGACATTGCGCTCTTGAAGATCACTCCTCCTGTTACATGTGGGAACTTC
17 Itgb7 Integrin beta-7 precursor (Integrin beta-P) (M290 IEL antigen). [Source:Uniprot/SWISSPROT;Acc:P26011] ENSMUSG00000001281 AC123791, S44607, BC011184, AC163291 TGACCTTGGCCCCTAACTTGGATGATGGCTGGTGCAAAGAAAGGACAATAGACAACCAGCTGTTCTTCT
18 Pde1b Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B (EC 3.1.4.17) (Cam-PDE 1B) (63 kDa Cam-PDE). [Source:Uniprot/SWISSPROT;Acc:Q01065] ENSMUSG00000022489 AC131721, AC108858, BC058531, AC167554, AC166244 GTACCCTGATCTAGATGGGTTTTTGACCCTTTGCCTCCTTCCTGTCCCCGGGAACTAGGAAGCCCTCAT
19 Frg1 FRG1 protein. [Source:Uniprot/SWISSPROT;Acc:P97376] ENSMUSG00000031590 BC002027 CACTGGACAATGGACTGTTTACACTGGGAGCTCCACACAGAGAAGTTGATGAAGGCCCCAGTCCTCCAG
20 Prkdc DNA-dependent protein kinase catalytic subunit (EC 2.7.1.37) (DNA- PKcs) (P460). [Source:Uniprot/SWISSPROT;Acc:P97313] ENSMUSG00000022672 D87521, D83786, AB030754, AC154586 AGGACCTCCTAGACCTTCGCTGACAGCAGTATATTTCTGACAGACAGAGGAAATCTACTTTATGGTGCT

Total number of rows: 19200

Table truncated, full table size 3627 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary data files not provided
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap