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Status
Public on Dec 15, 2009
Title
Plants 4K miRNA LC_MRA-1038_miRNAPlants_11.0 + Bn_PsRNAs
Technology type
in situ oligonucleotide
Distribution
custom-commercial
Organisms
Physcomitrium patens ; Pinus taeda ; Populus trichocarpa ; Arabidopsis thaliana ; Brassica napus ; Brassica rapa ; Brassica oleracea ; Glycine max ; Medicago truncatula ; Oryza sativa ; Saccharum officinarum ; Sorghum bicolor ; Triticum aestivum ; Zea mays ; Vitis vinifera ; Selaginella moellendorffii Manufacturer
LC Sciences
Manufacture protocol
Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/ ) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.
Support
silicon
Description
Plant miRNA microarray with 100% coverage of mature miRNAs listed in miRBase version 11.0 and additional custom small RNA sequences from Brassica napus phloem sap (Bn_PsRNAs)
Web link
http://www.LCsciences.com Contributor(s)
Buhtz A , Kehr J Submission date
Dec 02, 2009
Last update date
Dec 15, 2009
Contact name
Anja Buhtz
E-mail(s)
anja.buhtz@upm.es Organization name
Centro de Biotecnologia y Genomica de Plantas
Lab
280
Street address
Campus de Montegancedo, M40,km38
City
Madrid
ZIP/Postal code
28223
Country
Spain
Samples (3)
GSM507517 , GSM507528 , GSM507529 Series (1)
GSE20263 Small RNAs in different tissues and phloem of oilseed rape under nutrient stress conditions
Data table header descriptions ID Probe_ID Name of the probe on the chip
Target_Seq Target sequence (5’ to 3’) of the probe
Row Row of the probe position on the chip
Column Column of the probe position on the chip
Group General function of the particular probe on the chip: experimental/control
Comment Information of the sequence: mature miRNA/custom sequence
SEQUENCE sequence of probe
miRNA_ID
Data table ID Probe_ID Target_Seq Row Column Group Comment SEQUENCE miRNA_ID 1
Bn_PsRNA_01
UGAACAAGGUUUCCCAGAUGC
16
23
experimental
Custom Sequence
GCATCTGGGAAACCTTGTTCA
PsRNA_01
2
Bn_PsRNA_01
UGAACAAGGUUUCCCAGAUGC
52
13
experimental
Custom Sequence
GCATCTGGGAAACCTTGTTCA
PsRNA_01
3
Bn_PsRNA_01
UGAACAAGGUUUCCCAGAUGC
88
3
experimental
Custom Sequence
GCATCTGGGAAACCTTGTTCA
PsRNA_01
4
Bn_PsRNA_01
UGAACAAGGUUUCCCAGAUGC
108
23
experimental
Custom Sequence
GCATCTGGGAAACCTTGTTCA
PsRNA_01
5
Bn_PsRNA_01
UGAACAAGGUUUCCCAGAUGC
121
23
experimental
Custom Sequence
GCATCTGGGAAACCTTGTTCA
PsRNA_01
6
Bn_PsRNA_02
UUGAAAAACAGAGACAAUAAAGGC
11
29
experimental
Custom Sequence
GCCTTTATTGTCTCTGTTTTTCAA
PsRNA_02
7
Bn_PsRNA_02
UUGAAAAACAGAGACAAUAAAGGC
47
19
experimental
Custom Sequence
GCCTTTATTGTCTCTGTTTTTCAA
PsRNA_02
8
Bn_PsRNA_02
UUGAAAAACAGAGACAAUAAAGGC
83
9
experimental
Custom Sequence
GCCTTTATTGTCTCTGTTTTTCAA
PsRNA_02
9
Bn_PsRNA_02
UUGAAAAACAGAGACAAUAAAGGC
103
29
experimental
Custom Sequence
GCCTTTATTGTCTCTGTTTTTCAA
PsRNA_02
10
Bn_PsRNA_02
UUGAAAAACAGAGACAAUAAAGGC
116
29
experimental
Custom Sequence
GCCTTTATTGTCTCTGTTTTTCAA
PsRNA_02
11
Bn_PsRNA_03
CCAUGGGAAUCGAACUAGGGGUGC
10
26
experimental
Custom Sequence
GCACCCCTAGTTCGATTCCCATGG
PsRNA_03
12
Bn_PsRNA_03
CCAUGGGAAUCGAACUAGGGGUGC
46
16
experimental
Custom Sequence
GCACCCCTAGTTCGATTCCCATGG
PsRNA_03
13
Bn_PsRNA_03
CCAUGGGAAUCGAACUAGGGGUGC
82
6
experimental
Custom Sequence
GCACCCCTAGTTCGATTCCCATGG
PsRNA_03
14
Bn_PsRNA_03
CCAUGGGAAUCGAACUAGGGGUGC
102
26
experimental
Custom Sequence
GCACCCCTAGTTCGATTCCCATGG
PsRNA_03
15
Bn_PsRNA_03
CCAUGGGAAUCGAACUAGGGGUGC
115
26
experimental
Custom Sequence
GCACCCCTAGTTCGATTCCCATGG
PsRNA_03
16
Bn_PsRNA_04
AAUAGGGAGGGCAAGGAGUGC
16
27
experimental
Custom Sequence
GCACTCCTTGCCCTCCCTATT
PsRNA_04
17
Bn_PsRNA_04
AAUAGGGAGGGCAAGGAGUGC
52
17
experimental
Custom Sequence
GCACTCCTTGCCCTCCCTATT
PsRNA_04
18
Bn_PsRNA_04
AAUAGGGAGGGCAAGGAGUGC
88
7
experimental
Custom Sequence
GCACTCCTTGCCCTCCCTATT
PsRNA_04
19
Bn_PsRNA_04
AAUAGGGAGGGCAAGGAGUGC
108
27
experimental
Custom Sequence
GCACTCCTTGCCCTCCCTATT
PsRNA_04
20
Bn_PsRNA_04
AAUAGGGAGGGCAAGGAGUGC
121
27
experimental
Custom Sequence
GCACTCCTTGCCCTCCCTATT
PsRNA_04
3281 339 Kbytes .Supplementary file Size Download File type/resource GPL9738_sequences for Platform_miRNA_release_11.0_14Dec09.txt.gz
13.0 Kb
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