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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 27, 2017 |
| Title |
Neat1 is a p53-inducible lincRNA essential for transformation suppression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
p53 is a transcriptional activator that induces myriad target genes. However, those p53-inducible genes most critical for tumor suppression remain elusive. Here, we identify Neat1, a ncRNA constituent of paraspeckles, as a p53 target gene induced by mouse and human p53 in different cell types and by diverse stress signals. Using fibroblasts derived from Neat1-/- mice, we examine the functional role of Neat1 in the p53 pathway. We find that Neat1 is dispensable for cell-cycle arrest and apoptosis in response to genotoxic stress, but plays a crucial role in suppressing transformation in response to oncogenic signals. To determine the effects of Neat1 deficiency on cells, we used E1A;HRasV12-expressing wild-type and Neat1-/- mouse embryonic fibroblasts (MEFs) for RNA-sequencing analysis. This analysis revealed that Neat1 deficiency impacts the regulatory networks involved in nervous system development and axon guidance programs, as well as genes of the SWI/SNF complex and components of the pancreas development network. These findings suggest that the ability of Neat1 to globally regulate gene expression, with effects on diverse transcriptional programs, provides a potential mechanism for how Neat1 acts to suppress transformation and tumor initiation.
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| Overall design |
RNA-seq was performed to determine the differentially expressed genes in E1A;HRasV12-expressing wild-type and Neat1-/- mouse embryonic fibroblasts (MEFs) .
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| Contributor(s) |
Mello SS, Attardi LD |
| Citation(s) |
28698299 |
| Submission date |
Jun 15, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Stephano Spano Mello |
| Organization name |
Stanford University
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| Lab |
Attardi lab
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| Street address |
269 Campus Drive
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA390672 |
| SRA |
SRP109310 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100098_RAW.tar |
1000.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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