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| Status |
Public on Sep 13, 2019 |
| Title |
Human Transcriptome Array 2.0 (HTA) from healthy colonic, colorectal adenoma and colorectal cancer tissue |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Whole transcriptome expression levels of healthy colonic, colorectal adenoma and colorectal cancer biopsy samples were analyzed by HTA 2.0 microarrays
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| Overall design |
Biopsy samples were collected from 20 healthy colonic, 20 colorectal adenoma and 20 colorectal cancer tissue during routine colonoscopy. HTA 2.0 microarray experiments were performed according to the manufacturer’s instructions. 100 ng input total RNA samples were applied as staring material. For sscDNA synthesis 15 µg cRNA was used and 5.5 µg fragmented and labeled sscDNA sample was hybridized to HTA 2.0 microarrays (Affymetrix) for 16 hours at 45°C with 60 rpm rotation in the Hybridization Oven (Affymetrix). Microarrays were washed and stained with GeneChip® Hybridization, Wash, and Stain Kit reagents according to FS450_0001 protocol using the Fluidics Station 450 instrument (Affymetrix). Scanning was performed with GeneChip Scanner 3000 (Affymetrix).
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| Contributor(s) |
Kalmár A, Brigitta Nagy Z, Galamb O, Csabai I, Bodor A, Wichmann B, Valcz G, Kinga Barták B, Tulassay Z, Igaz P, Molnar B |
| Citation(s) |
35655145 |
| Submission date |
Jun 19, 2017 |
| Last update date |
Jun 23, 2022 |
| Contact name |
Wichmann Barna |
| E-mail(s) |
wwbarna@yahoo.com
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| Organization name |
SOTE
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| Street address |
Szentkirály
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| City |
Budapest |
| ZIP/Postal code |
1081 |
| Country |
Hungary |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (60)
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| Relations |
| BioProject |
PRJNA390932 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100179_2017-07-21-annotated-RMA-SKETCH.RMA-GENE-FULL-Group1.txt.gz |
23.0 Mb |
(ftp)(http) |
TXT |
| GSE100179_RAW.tar |
1.5 Gb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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