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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 21, 2018 |
| Title |
Natural and molecular history of prolactinoma: insights from a Prlr -/- mouse model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mouse models of prolactinoma can be useful to better understand molecular mechanisms involved in abnormal lactotroph cell proliferation and secretion. We have previously developed a prolactin receptor deficient (Prlr -/-) mouse, in which Prlr -/- females develop large secreting prolactinomas from 12 months of age with a penetrance of 100%, mimicking human aggressive densely granulated macroprolactinoma, a highly secreting subtype.
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| Overall design |
In order to gain molecular insights into the pathogenesis of prolactinoma in Prlr-/- mice, we performed a microarray analysis at 2 and 4 months of age, before the overt appearance of the tumors. Two and four months-old wild-type (n=6 and 6 respectively) and Prlr KO (n=6 and 6 respectively) mice were sacrified by decapitation. Pituitaries were removed, pooled three by three to form duplicates for each genotype, and frozen at -80°C before RNA extraction and subsequent analysis. The whole experiment was replicated with another set of animals (12 wild-type and 12 Prlr -/- mice) (Note : in the replication experiment (array2), the second duplicate for 4 months-old mice did not hybridize correctly and therefore was removed before analysis, both in the WT and the Prlr KO conditions)
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| Contributor(s) |
Bernard V, Villa C, Auguste A, Lamothe S, Guillou A, Martin A, Caburet S, Young J, Veitia RA, Binart N |
| Citation(s) |
29464061 |
| Submission date |
Jun 21, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Nadine Binart |
| E-mail(s) |
nadine.binart@inserm.fr
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| Organization name |
INSERM 1185
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| Department |
Faculté de médecine Paris Sud
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| Street address |
63 rue Gabriel Péri
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| City |
le Kremlin-Bicêtre |
| ZIP/Postal code |
94276 |
| Country |
France |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA391348 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100334_RAW.tar |
44.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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