|
Status |
Public on Jun 22, 2017 |
Title |
Expression profiling of lncRNAs, miRNAs and mRNAs and their differential expression in leiomyoma using next generation RNA sequencing |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing
|
Summary |
Total RNA was isolated from leiomyoma and paired myometrium (N=8) and samples from three pairs were subjected to RNA sequencing. After analysis, 5941 lncRNAs (2813 up- and 3128 down-regulated at ≥1.5 fold), 148 miRNAs (56 up- and 96 down-regulated at ≥1.5 fold) and 3855 mRNAs (2030 up- and 1855 down-regulated at ≥1.5 fold) were differentially expressed in leiomyomas. Using QRT-PCR we further confirmed the expression of HULC, lnc-MEG3, LINC00890, TSIX, LINC00473, lnc-KLF9-1 and lnc-POTEM-3 (lncRNA-ATB) in leiomyoma and matched myometrium (N=8). Our results presented here provide a comprehensive expression profile of lncRNAs in leiomyomas with concurrent integrated expression of miRNAs and mRNAs in leiomyomas.
|
|
|
Overall design |
Identify the expression profile of lncRNAs with concurrent assessment of miRNAs and mRNAs profiles in leiomyomas and paired myometrium using next generation RNA sequencing
|
|
|
Contributor(s) |
Chuang T, Khorram O |
Citation(s) |
28587571 |
Submission date |
Jun 21, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Tsai-Der Chuang |
E-mail(s) |
chuangtsaide@gmail.com
|
Phone |
310-222-1897
|
Organization name |
LA Biomed at UCLA medical center
|
Department |
OB-GYN
|
Lab |
Omid Khorram
|
Street address |
1124 WEST CARSON STREET
|
City |
Torrance |
State/province |
California |
ZIP/Postal code |
90502 |
Country |
USA |
|
|
Platforms (1) |
GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
Samples (6)
|
|
Relations |
BioProject |
PRJNA391373 |
SRA |
SRP110081 |
Supplementary file |
Size |
Download |
File type/resource |
GSE100338_CAP_miRSeq_count_miRNA_.txt.gz |
14.9 Kb |
(ftp)(http) |
TXT |
GSE100338_TMM_normalized_reads_count_per_million_lncRNA_.txt.gz |
778.2 Kb |
(ftp)(http) |
TXT |
GSE100338_TMM_normalized_reads_count_per_million_mRNA_.txt.gz |
951.7 Kb |
(ftp)(http) |
TXT |
GSE100338_TMM_normalized_reads_count_per_million_miRNA_.txt.gz |
33.0 Kb |
(ftp)(http) |
TXT |
GSE100338_X183_FC1.5_pVal0.05_15_genes.txt.gz |
1.1 Kb |
(ftp)(http) |
TXT |
GSE100338_X183_FC1.5_pVal0.05_520_genes.txt.gz |
27.5 Kb |
(ftp)(http) |
TXT |
GSE100338_X183_FC1.5_pVal0.05_913_genes.txt.gz |
66.6 Kb |
(ftp)(http) |
TXT |
GSE100338_X189_FC1.5_pVal0.05_1128_genes.txt.gz |
82.0 Kb |
(ftp)(http) |
TXT |
GSE100338_X189_FC1.5_pVal0.05_42_genes.txt.gz |
2.8 Kb |
(ftp)(http) |
TXT |
GSE100338_X189_FC1.5_pVal0.05_792_genes.txt.gz |
38.5 Kb |
(ftp)(http) |
TXT |
GSE100338_X190_FC1.5_pVal0.05_12_genes.txt.gz |
950 b |
(ftp)(http) |
TXT |
GSE100338_X190_FC1.5_pVal0.05_414_genes.txt.gz |
19.3 Kb |
(ftp)(http) |
TXT |
GSE100338_X190_FC1.5_pVal0.05_470_genes.txt.gz |
34.8 Kb |
(ftp)(http) |
TXT |
GSE100338_rsem_count_lncRNA_.txt.gz |
541.2 Kb |
(ftp)(http) |
TXT |
GSE100338_rsem_count_mRNA_.txt.gz |
361.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |