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| Status |
Public on Oct 13, 2018 |
| Title |
Integrated Transcriptomic and Proteomic Dynamics of Rituximab Treatment in Primary B Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The investigation aims to profile the molecular dynamics of Rituximab treatment in cells. Specifically, primary B cells were treated with Rituximab, and sampled across 6 time points. Treated and untreated cells were processed to extract RNA and protein, that were subsequently used in high-throughput analysis of the transcriptome (RNA-Sequencing) and proteome (mass spectrometry).
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| Overall design |
Examination of gene expression dynamics in two sets of: (i) treated Primary B cells and (ii) a parallel untreated control primary B cells. Both sets were sampled at 6 timepoints - (0, 1, 2, 4, 7, and 15 hours, with respect to the start of the experiment). The treated cells, (i), were treated with Rituximab at timepoint 1 (0 hours). Validation was performed using a new repeated experiment over the same time points, including again both treatment and control sets of primary B cells.
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| Contributor(s) |
Mias GI, Brooks LR |
| Citation missing |
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| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R00 HG007065 |
Integrative Dynamic Omics Profiling: A First Step Towards Personalized Medicine |
MICHIGAN STATE UNIVERSITY |
George Ioannou Mias |
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| Submission date |
Jun 23, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
George Mias |
| Organization name |
Michigan State University
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| Department |
Biochemistry and Molecular Biology
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| Street address |
775 Woodlot Drive, Rm1319
|
| City |
East Lansing |
| ZIP/Postal code |
48824 |
| Country |
USA |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA391743 |
| SRA |
SRP110313 |
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