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| Status |
Public on Jun 13, 2018 |
| Title |
mRNA profiles of JMJD3 overexpression- and JMJD3 knockout- HL-60 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Next Generation Sequencing Facilitates Quantitative Analysis of HL-60 cells transduced with control or JMJD3-expression vector, and HL-60 parental and JMJD3 knockout cells
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| Overall design |
mRNA profiles of HL-60 cells were generated by deep sequencing, in triplicate, using Illumina HiSeq 4000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using SYBR Green assays.
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| Contributor(s) |
Yu S, Zhu J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 26, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
wang yujie |
| E-mail(s) |
wangyj@rainbow-genome.com
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| Phone |
+86 18200353339
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| Organization name |
Shanghai JiaYin Biological technology co., LTD
|
| Street address |
Chi Feng road No:65
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| City |
shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200082 |
| Country |
China |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE101891 |
JMJD3 facilitates C/EBPβ-centered transcriptional program to exert oncorepressor activity in AML |
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| Relations |
| BioProject |
PRJNA391934 |
| SRA |
SRP110517 |
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