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| Status |
Public on Jan 01, 2018 |
| Title |
Gene expression from total and polysomal RNA fractions after ATO treatment in LN229 cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Arsenic trioxide (ATO) treatment leads to activation of mRNA translation through the MAPK-interacting kinase (MNK) signaling pathway. Polysomal fractionation and microarray analysis allows for identification of transcripts undergoing active translation. We identified the genes differentially enriched in untreated and ATO treated fractions.
In this dataset, we include expression data in untreated and ATO treated LN229 cells using either the total or polysomal RNA.
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| Overall design |
LN229 cells were untreated or treated with ATO. Cells were lysed and total RNA was collected. Total RNA was then subjected to polysomal fractionation. Each sample was performed in technical triplicates. Data was then pooled and analyzed using the Transcriptome Analysis Console (TAC) from Affymetrix. Coding genes with >1.5 increase in polysomal vs total RNA were then compared for untreated and ATO treated groups to generate lists of genes enriched in polysomal fractions.
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| Contributor(s) |
Bell JB, Platanias LC |
| Citation(s) |
29042487 |
| Submission date |
Jun 27, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Matthew J. Schipma |
| E-mail(s) |
m-schipma@northwestern.edu
|
| Phone |
1-312-503-5324
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| Organization name |
Northwestern University
|
| Department |
Biochemistry and Molecular Genetics
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| Street address |
750 N. Lake Shore Drive
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (1) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA392415 |
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