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| Status |
Public on Jan 25, 2018 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of Bone Marrow Derived Mast Cell Transcriptomes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose:The purpose of this study is to detect activated or silenced genes during Tet2-deficient bone marrow derived mast cell (BMMC) and the control cells. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods:Mouse BMMCs were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse IL-3 and SCF. BMMCs were stained to confirm the surface expression of FcɛRI and c-Kit. Cells with purity >97.5% were used for subsequent experiments. BMMCs RNA profiles were generated by deep sequencing,using Illumina. Results: We mapped about 10 million sequence reads per sample to the mouse genome, identified hundreds of genes with significant mRNA variation between Tet2-deficient BMMC and the control cells.
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| Overall design |
BMMC mRNA profiles of wild type (WT) and Tet2-/- mice were generated by deep sequencing.
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| Contributor(s) |
Shen Q, Cao X |
| Citation(s) |
29364877 |
| Submission date |
Jun 27, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Qian Zhang |
| E-mail(s) |
oilpangpang@hotmail.com
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| Phone |
+8615921196443
|
| Organization name |
Second military medical university
|
| Street address |
xiangyin road 800
|
| City |
shang hai |
| ZIP/Postal code |
200433 |
| Country |
China |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA392109 |
| SRA |
SRP110608 |
