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| Status |
Public on Oct 08, 2017 |
| Title |
Growth differentiation factor 15 (GDF15) promotes human rhinovirus infection and inflammation |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Analysis of normal human tracheobronchial epithelial cells treated with or without recombinant human GDF15 for two hours. Results provide insights of the genome-wide transcriptional regulation by Smad1 associated with GDF15 in human airway epithelium.
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| Overall design |
Normal human tracheobronchial epithelial cells at passage 2 were seeded onto collagen-coated 60 mm tissue culture dishes (4 x105 cells) in bronchial epithelial cell growth medium (BEGM) with SingleQuots™ supplements (Lonza, Walkersville, MD) at 37°C, 5% CO2. At 80-90% confluence, cells were treated with 0.1% BSA-HCl (control) or 25 ng/ml of recombinant human GDF15 (rhGDF15, R&D Systems, Minneapolis, MN) for 2 h.
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| Contributor(s) |
OConnor BP, Harmacek LD, Wu Q, Leach S |
| Citation(s) |
29192094 |
| Submission date |
Jun 28, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Brian Patrick OConnor |
| E-mail(s) |
oconnorb@njhealth.org
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| Organization name |
National Jewish Health
|
| Department |
Center for Genes, Environment and Health
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| Lab |
OConnor
|
| Street address |
1400 Jackson St
|
| City |
Denver |
| State/province |
Colorado |
| ZIP/Postal code |
80206 |
| Country |
USA |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA392313 |
| SRA |
SRP110705 |
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