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| Status |
Public on Apr 11, 2018 |
| Title |
MicroRNA profiling of demyelination and remyelination areas in corpus callosum of cuprizone-treated mice |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Mouse cuprizone (CPZ ) model of experimental de- and remyelination was applied to mimic demyelination pathology of multiple sclerosis. In order to identify differentially expressed microRNAs involved in de- and remyelination, the affected areas of corpus callosum were isolated from mice exposed to CPZ and conducted an Agilent microarray analysis. To induce demyelination, CPZ was administrated for four weeks. Spontaneous remyelination occurs as mice returned to the regular diet after four weeks feeding with CPZ (DEM_4w). Remyelination was examined at two time points: acute remyelination induced by four weeks CPZ feeding followed by two days of regular diet (two days remyelination: REM_2d), and full remyelination induced by four weeks CPZ feeding followed by two weeks of regular diet (two weeks remyelination: REM_2w). Control mice (C) were kept on a normal diet.
The following groups representing de- and remyelinisation pathology in corpus callosum of CPZ-treated mice were compared: Demyelination: 4weeks CPZ: DEM_4w; Acute remyelination: 4 weeks CPZ +2 days UNTREATED: REM_2d; Full remyelination: 4 weeks CPZ +2 weeks UNTREATED: REM_2w; and UNTREATED control (C). The experiments were performed using 2-4 animals per groups.
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| Overall design |
Nellie, A,Martin
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| Contributor(s) |
Molnar V, Somogyi GT, Elkjaer ML, Nawrocki A, Okarmus J, Wlodarczyk A, Thygesen EK, Palkovits M, Gallyas F, Larsen MR, Lassmann H, Benedikz E, Owens T, Svenningsen AF, Illes Z |
| Citation(s) |
29593734 |
| Submission date |
Jun 29, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Viktor Molnár |
| E-mail(s) |
molvik.dgci@gmail.com
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| Organization name |
Semmelweis University
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| Department |
Department of Genetics, Cell- and Immunobiology
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| Street address |
Nagyvárad tér 4.
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| City |
Budapest |
| ZIP/Postal code |
1089 |
| Country |
Hungary |
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| Platforms (1) |
| GPL8824 |
Agilent-019119 Mouse miRNA Microarray 1.0 G4472A (miRNA ID version) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA392450 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100662_RAW.tar |
9.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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