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| Status |
Public on Jul 06, 2017 |
| Title |
Ebola virus (EBOV) infection of ARPE-19 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We inoculated ARPE-19 human retinal pigment epithelial cells with EBOV, and followed course of infection by immunocytochemistry and measurement of titer in culture supernatant. To interrogate transcriptional responses of infected cells, we combined RNA sequencing with in silico pathway, gene ontology, transcription factor binding site and network analyses. Human retinal pigment epithelial cells were permissive to infection with EBOV, and supported viral replication and release of virus in high titer. Unexpectedly, 28% of 560 up-regulated transcripts in EBOV-infected cells were type I IFN responsive, indicating a robust type I IFN response.
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| Overall design |
Transcriptomic profiles of ARPE-19 cells 24 hours following infection with EBOV or mock-infection
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| Contributor(s) |
Smith J, Michael M |
| Citation(s) |
28721309 |
| Submission date |
Jul 05, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Justine Smith |
| Organization name |
Flinders University
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| Street address |
Bedford Park
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| City |
Adelaide |
| ZIP/Postal code |
5042 |
| Country |
Australia |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA393207 |
| SRA |
SRP111145 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100839_Read_Counts_Non_Normalized.txt.xlsx |
1.1 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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