|
| Status |
Public on Aug 10, 2017 |
| Title |
Microsatellite expansion RNA visualization, elimination, and reversal of molecular pathology by RNA-targeting Cas9 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this Study, we used RNA-targeting Cas9 (RCas9) to reverse characteristic Myotonic Dystrophy (DM1) cellular phenotypes such as elimination of RNA foci, MBNL relocalization, and reversal of transcriptome-wide splicing.
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| |
|
| Overall design |
We performed RNA-seq for control and myotonic dystrophy (DM1) myoblasts and myotubes with various treatments including lentivirus mediated expression of either non-targeting (NT) or CTG-targeting (CTG) single guide RNA (sgRNA) and PIN-dCas9.Lentivirus mediated GFP expression was used as control.
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| |
|
| Contributor(s) |
Batra R, Yeo G |
| Citation(s) |
28803727 |
| Submission date |
Jul 07, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Ranjan Batra |
| E-mail(s) |
ranjanbatra9@yahoo.com
|
| Phone |
352-222-9209
|
| Organization name |
University of California San Diego
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Yeo Lab
|
| Street address |
2880 Torrey Pines Scenic Drive
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (9)
|
| GSM2696925 |
myoblast_Myotonic Dystriphy type I (DM1) non targeting |
| GSM2696926 |
myoblast_Myotonic Dystriphy type I (DM1) ctg targeting |
| GSM2696927 |
myotube_Control-Non targeting |
| GSM2696928 |
myotube_Myotonic Dystriphy type I (DM1) non targeting |
| GSM2696929 |
myotube_Control-CTG targeting |
| GSM2696930 |
myotube_Myotonic Dystriphy type I (DM1) ctg targeting |
|
| Relations |
| BioProject |
PRJNA393468 |
| SRA |
SRP111361 |
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