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| Status |
Public on Apr 18, 2018 |
| Title |
Gene Expression in the BAT and WAT of ob/ob and ob/ob/Cidea-/-/Cidec-/- mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
ob/ob mice is an obese mice. CIDE family proteins including Cidea, Cideb and Cidec play important role in lipid metabolism. Cidea is mainly expressed in the brown adipose tissue (BAT). Cidec is mainly expressed in the BAT and white adipose tissue (WAT). We generated ob/ob/Cidea-/-/Cidec-/- mice to investigate the phenotype of fat tissue. ob/ob/Cidea-/-/Cidec-/- mice are lean when compared with ob/ob mice. The tissue weight and TAG content of BAT and WAT was extreamly decreased in ob/ob/Cidea-/-/Cidec-/- mice compared with that in ob/ob mice.
We next extract the total RNA from the BAT and WAT of ob/ob and ob/ob/Cidea-/-/Cidec-/- mice, to perform microarray analysis using Mouse Gene 1.0 ST array system, Affymetrix. We then analysised the up-regulated and down regulated pathways.
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| Overall design |
We extract the RNA of BAT and WAT from 4 month old mice and hybridization on Affymetrix microarrays. We then analysis the data.
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| Contributor(s) |
Zhou L, Xia X, Gu Y |
| Citation(s) |
29986925 |
| Submission date |
Jul 09, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Linkang Zhou |
| E-mail(s) |
zhoulinkang@gmail.com
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| Phone |
86-15811043559
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| Organization name |
Tsinghua Unversity
|
| Street address |
Haidian District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA393576 |
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