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| Status |
Public on Feb 22, 2020 |
| Title |
Overexpression of nuclear receptor 5A1 induces and maintains an intermediate state of conversion between primed and naive pluripotency |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Naive and primed human pluripotent stem cells (hPSCs) have provided useful insights into the regulation of pluripotency. However, the molecular mechanisms regulating naive conversion remain elusive. Here, we report intermediate naive conversion induced by overexpressing nuclear receptor 5A1 (NR5A1) in hPSCs. The cells displayed some naive features, such as clonogenicity, glycogen synthase kinase 3β and mitogen activated protein kinase (MAPK) independence, expression of naive-associated genes, and two activated X chromosomes, but lacked others such as KLF17 expression, transforming growth factor β independence, and imprinted gene demethylation. Notably, NR5A1 negated MAPK activation by fibroblast growth factor 2, leading to cell-autonomous self-renewal independent of MAPK inhibition. These phenotypes may be associated with naive conversion, and were regulated by a DPPA2/4-dependent pathway that activates the selective expression of naive-associated genes. This study increases our understanding of the mechanisms regulating the conversion from primed to naive pluripotency.
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| Overall design |
Examination of two distinct pluripotent states, primed and naive, with ChIP-seq of H3K4me3, H3K27me3, and NR5A1 (FLAG)
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| Contributor(s) |
Yamauchi K, Ikeda T, Hosokawa M, Nakatsuji N, Kawase E, Chuma S, Hasegawa K, Suemori H |
| Citation(s) |
32084386 |
| Submission date |
Jul 10, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Hirofumi Suemori |
| E-mail(s) |
hsuemori@infront.kyoto-u.ac.jp
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| Phone |
81-75-751-3821
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| Organization name |
Kyoto University
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| Department |
Institute for Frontier Life and Medical Sciences
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| Lab |
Embryonic Stem Cell Research
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| Street address |
53 Kawaharacho, Shogoin, Sakyo-ku
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| City |
Kyoto |
| ZIP/Postal code |
606-8505 |
| Country |
Japan |
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| Platforms (1) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE101089 |
Overexpression of nuclear receptor 5A1 induces and maintains an intermediate state of conversion between primed and naive pluripotency |
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| Relations |
| BioProject |
PRJNA393695 |
| SRA |
SRP111465 |
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