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Series GSE10123 Query DataSets for GSE10123
Status Public on Feb 09, 2008
Title Progerin-induced changes in gene expression
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The premature aging disease Hutchinson-Gilford Progeria Syndrome (HGPS) is caused by constitutive production of progerin, a mutant form of the nuclear architectural protein lamin A1. Progerin is also sporadically expressed in wild type cells and has been linked to physiological aging. HGPS cells exhibit extensive nuclear defects including abnormal chromatin structure and increased DNA damage. At the organismal level, HGPS affects several tissues particularly of mesenchymal origin. How the cellular defects of HGPS cells lead to the organismal defects has been unclear. To begin to unravel how progerin leads to disease phenotypes, we analyzed time-dependent changes in transcriptional profiles in response to progerin expression in a hTERT-immortalized skin fibroblast cell line expressing either GFP-progerin or the GFP-wt lamin A control
Keywords: time course, cell line comparison
 
Overall design Three time points (0d, 5d, 10d after induction of the exogenous protein) were analyzed for each cell line (expressing either GFP-progerin or GFP-wt lamin A). Two biological replicates were analyzed for each time point.
 
Contributor(s) Scaffid P, Misteli T
Citation(s) 18311132
Submission date Jan 09, 2008
Last update date Oct 28, 2014
Contact name paola scaffidi
E-mail(s) scaffidp@mail.nih.gov
Organization name NCI, NIH
Street address 41 Library Drive, B508
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL2895 GE Healthcare/Amersham Biosciences CodeLink Human Whole Genome Bioarray
Samples (12)
GSM255774 WT-GFP-lamin A Induction: Day 0 Replicate A (CBGE_Hsapiens_WG_progerin1)
GSM255806 WT-GFP-lamin A Induction: day 0 Replicate B (1b_WT_0d_B)
GSM255807 WT-GFP-lamin A Induction: day 5 Replicate A (2a_WT_5d_A)
Relations
BioProject PRJNA108667

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
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Supplementary data files not provided
Raw data included within Sample table
Processed data included within Sample table

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