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| Status |
Public on Apr 01, 2008 |
| Title |
Analysis of Endoglin function in rat hepatic stellate cells (HSC) |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Endoglin is a transmembrane receptor able to bind several members of the transforming growth factor-beta superfamliy. We used a siRNA-mediated knock down approach to analyse the function of Endoglin in hepatic stellate cells (HSC). We found that the targeted disruption is associated with significant changes in gene expression resulting in cellular changes that prolongated cellular activation, morphological maturation, transdifferentiation, expression of basement and fibrillar extracellular matrix proteins. In addition, the lack of Endoglin resulted in rearrangements of cytoskeletal organization.
Keywords: gene function analysis, siRNA, gene knock down
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| Overall design |
We have performed a set of four microarray hybridizations using Agilent Whole Rat Genome Oligo Microarrays. We compartively analysed the gene expression profile in RNAs taken from: (I) untreated HSC taken from one animal vs. HSC that were taken from one animal and transfected with a control siRNA, (II) HSC taken from one animal and transfected with a siRNA targeting endoglin vs. HSC that were taken from one animal and transfected with a control siRNA, (III) untreated HSC that were pooled from three individual animals vs. HSC that were taken from three individual animals and independently transfected with siRNA control, and finally (IV) HSC that were taken from three animals and independently transfected with a siRNA targeting endoglin vs HSC that were taken from three individual animals and independently transfected with a siRNA control.
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| Contributor(s) |
Vreden WN, Meurer SK, Weiskirchen R |
| Citation missing |
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| Submission date |
Jan 11, 2008 |
| Last update date |
Jul 17, 2017 |
| Contact name |
Ralf Weiskirchen |
| E-mail(s) |
rweiskirchen@ukaachen.de
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| Phone |
+49-241-8088683
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| Fax |
+49-241-8082512
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| URL |
http://www.ukaachen.de/content/folder/5095184
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| Organization name |
RWTH University Hospital Aachen
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| Department |
Institute of Clinical Chemistry and Pathobiochemistry
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| Street address |
Pauwelsstr. 30
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| City |
Aachen |
| State/province |
NRW |
| ZIP/Postal code |
52074 |
| Country |
Germany |
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| Platforms (1) |
| GPL4135 |
Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA108219 |
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